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α-Linolenic acid (ALA) is an anti-inflammatory agent in inflammatory bowel disease.
J Nutr Biochem. 2015 Dec; 26(12):1632-40.JN

Abstract

Studies suggest that consumption of omega-3 (n-3) polyunsaturated fatty acids (PUFA) plays a protective role in inflammatory bowel disease; however, the use of plant-derived oils rich in α-linolenic acid (ALA) has not been widely investigated. The aims of this study were to test the effects of two different sources of (n-3) PUFA, fish and plant-derived oils, in two animal models of experimental colitis and to determine whether the (n-3) PUFA-enriched diets could ameliorate the inflammatory status. Rats were fed diets rich in corn, fish or sage oil with or without vitamin A supplementation for 3weeks then colitis was induced by adding dextran sodium sulfate to the drinking water or by injecting 2,4,6-trinitrobenzene sulfonic acid. We show that colitic rats fed the sage oil diets had a lower inflammatory response, improved histological repair and had less necrotic damage in the mucosa when compared to the corn and fish oil groups. Colonic damage and myeloperoxidase activity were significantly lower. Colonic mRNA levels of pro-inflammatory genes including interleukin IL-6, cyclooxygenase 2 and tumor necrosis factor α were markedly down-regulated in rats fed fish and sage oils compared to control. These results were supported by experiments in the human colonic epithelial cell line Caco-2, where ALA supplementation was shown to be effective in inhibiting inflammation induced by IL-1β by down-regulating mRNA levels of pro-inflammatory genes including IL-8, COX2 and inducible nitric oxide synthase. Taken together, these results suggest that plant-derived oil rich in ALA could ameliorate the inflammatory damage in colitis.

Authors+Show Affiliations

The School of Nutritional Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel. Electronic address: ram.reifen@mail.huji.ac.il.The School of Nutritional Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.The School of Nutritional Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.The School of Nutritional Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26350254

Citation

Reifen, Ram, et al. "Α-Linolenic Acid (ALA) Is an Anti-inflammatory Agent in Inflammatory Bowel Disease." The Journal of Nutritional Biochemistry, vol. 26, no. 12, 2015, pp. 1632-40.
Reifen R, Karlinsky A, Stark AH, et al. Α-Linolenic acid (ALA) is an anti-inflammatory agent in inflammatory bowel disease. J Nutr Biochem. 2015;26(12):1632-40.
Reifen, R., Karlinsky, A., Stark, A. H., Berkovich, Z., & Nyska, A. (2015). Α-Linolenic acid (ALA) is an anti-inflammatory agent in inflammatory bowel disease. The Journal of Nutritional Biochemistry, 26(12), 1632-40. https://doi.org/10.1016/j.jnutbio.2015.08.006
Reifen R, et al. Α-Linolenic Acid (ALA) Is an Anti-inflammatory Agent in Inflammatory Bowel Disease. J Nutr Biochem. 2015;26(12):1632-40. PubMed PMID: 26350254.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - α-Linolenic acid (ALA) is an anti-inflammatory agent in inflammatory bowel disease. AU - Reifen,Ram, AU - Karlinsky,Anna, AU - Stark,Aliza H, AU - Berkovich,Zipi, AU - Nyska,Abraham, Y1 - 2015/08/14/ PY - 2015/03/02/received PY - 2015/08/05/revised PY - 2015/08/05/accepted PY - 2015/9/10/entrez PY - 2015/9/10/pubmed PY - 2016/12/15/medline KW - Alpha-linolenic acid KW - Colitis KW - Inflammatory bowel disease KW - Omega-3 KW - Vitamin A SP - 1632 EP - 40 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 26 IS - 12 N2 - Studies suggest that consumption of omega-3 (n-3) polyunsaturated fatty acids (PUFA) plays a protective role in inflammatory bowel disease; however, the use of plant-derived oils rich in α-linolenic acid (ALA) has not been widely investigated. The aims of this study were to test the effects of two different sources of (n-3) PUFA, fish and plant-derived oils, in two animal models of experimental colitis and to determine whether the (n-3) PUFA-enriched diets could ameliorate the inflammatory status. Rats were fed diets rich in corn, fish or sage oil with or without vitamin A supplementation for 3weeks then colitis was induced by adding dextran sodium sulfate to the drinking water or by injecting 2,4,6-trinitrobenzene sulfonic acid. We show that colitic rats fed the sage oil diets had a lower inflammatory response, improved histological repair and had less necrotic damage in the mucosa when compared to the corn and fish oil groups. Colonic damage and myeloperoxidase activity were significantly lower. Colonic mRNA levels of pro-inflammatory genes including interleukin IL-6, cyclooxygenase 2 and tumor necrosis factor α were markedly down-regulated in rats fed fish and sage oils compared to control. These results were supported by experiments in the human colonic epithelial cell line Caco-2, where ALA supplementation was shown to be effective in inhibiting inflammation induced by IL-1β by down-regulating mRNA levels of pro-inflammatory genes including IL-8, COX2 and inducible nitric oxide synthase. Taken together, these results suggest that plant-derived oil rich in ALA could ameliorate the inflammatory damage in colitis. SN - 1873-4847 UR - https://www.unboundmedicine.com/medline/citation/26350254/α_Linolenic_acid__ALA__is_an_anti_inflammatory_agent_in_inflammatory_bowel_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(15)00200-4 DB - PRIME DP - Unbound Medicine ER -