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Antioxidant action of six Trifolium species in blood platelet experimental system in vitro.
Mol Cell Biochem. 2015 Dec; 410(1-2):229-37.MC

Abstract

This study includes a comparative evaluation of antioxidant effects of plant extracts (1.5-50.0 μg/ml), derived from six clover (Trifolium) species: T. alexandrinum L., T. fragiferum L., T. hybridum L., T. incarnatum L., T. resupinatum var. majus Boiss., and T. resupinatum var. resupinatum L. Chemical profiles of the extracts contained three or four groups of (poly)phenolic compounds such as phenolic acids, clovamides, isoflavones, and other flavonoids. Antioxidant properties of Trifolium extracts were assessed as the efficacy to reduce oxidative and nitrative damage to blood platelets, exposed to 100 μM peroxynitrite-induced oxidative stress in vitro. Antioxidant actions of the examined extracts were determined by the following biomarkers of oxidative stress: thiol groups, 3-nitrotyrosine, lipid hydroperoxides, and thiobarbituric acid-reactive substances (TBARS). Despite the significant differences in the chemical composition (the total phenolic concentrations varied between 11.30 and 52.55 mg/g of dry mass) of Trifolium extracts, we observed noticeable protective effects of almost all tested plant preparations. The T. alexandrinum extract, containing the highest concentration of phenols, was the most effective antioxidant among the tested extracts. On the other hand, the T. incarnatum extract, which contained a comparable total phenolic content (49.77 mg/g), was less efficient in prevention of tyrosine nitration and generation of TBARS. These findings indicate on the important role of individual phenolic components of the examined clover extracts for the final antioxidative effects. Antioxidative properties of the remaining extracts were noticeably weaker.

Authors+Show Affiliations

Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland. joannak@biol.uni.lodz.pl.Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland.Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, 24-100, Pulawy, Poland.Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, 24-100, Pulawy, Poland.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26350568

Citation

Kolodziejczyk-Czepas, J, et al. "Antioxidant Action of Six Trifolium Species in Blood Platelet Experimental System in Vitro." Molecular and Cellular Biochemistry, vol. 410, no. 1-2, 2015, pp. 229-37.
Kolodziejczyk-Czepas J, Nowak P, Kowalska I, et al. Antioxidant action of six Trifolium species in blood platelet experimental system in vitro. Mol Cell Biochem. 2015;410(1-2):229-37.
Kolodziejczyk-Czepas, J., Nowak, P., Kowalska, I., & Stochmal, A. (2015). Antioxidant action of six Trifolium species in blood platelet experimental system in vitro. Molecular and Cellular Biochemistry, 410(1-2), 229-37. https://doi.org/10.1007/s11010-015-2556-2
Kolodziejczyk-Czepas J, et al. Antioxidant Action of Six Trifolium Species in Blood Platelet Experimental System in Vitro. Mol Cell Biochem. 2015;410(1-2):229-37. PubMed PMID: 26350568.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant action of six Trifolium species in blood platelet experimental system in vitro. AU - Kolodziejczyk-Czepas,J, AU - Nowak,P, AU - Kowalska,I, AU - Stochmal,A, Y1 - 2015/09/08/ PY - 2015/05/28/received PY - 2015/09/03/accepted PY - 2015/9/10/entrez PY - 2015/9/10/pubmed PY - 2016/8/16/medline KW - Antioxidant KW - Blood platelets KW - Clover KW - Oxidative stress KW - Trifolium SP - 229 EP - 37 JF - Molecular and cellular biochemistry JO - Mol Cell Biochem VL - 410 IS - 1-2 N2 - This study includes a comparative evaluation of antioxidant effects of plant extracts (1.5-50.0 μg/ml), derived from six clover (Trifolium) species: T. alexandrinum L., T. fragiferum L., T. hybridum L., T. incarnatum L., T. resupinatum var. majus Boiss., and T. resupinatum var. resupinatum L. Chemical profiles of the extracts contained three or four groups of (poly)phenolic compounds such as phenolic acids, clovamides, isoflavones, and other flavonoids. Antioxidant properties of Trifolium extracts were assessed as the efficacy to reduce oxidative and nitrative damage to blood platelets, exposed to 100 μM peroxynitrite-induced oxidative stress in vitro. Antioxidant actions of the examined extracts were determined by the following biomarkers of oxidative stress: thiol groups, 3-nitrotyrosine, lipid hydroperoxides, and thiobarbituric acid-reactive substances (TBARS). Despite the significant differences in the chemical composition (the total phenolic concentrations varied between 11.30 and 52.55 mg/g of dry mass) of Trifolium extracts, we observed noticeable protective effects of almost all tested plant preparations. The T. alexandrinum extract, containing the highest concentration of phenols, was the most effective antioxidant among the tested extracts. On the other hand, the T. incarnatum extract, which contained a comparable total phenolic content (49.77 mg/g), was less efficient in prevention of tyrosine nitration and generation of TBARS. These findings indicate on the important role of individual phenolic components of the examined clover extracts for the final antioxidative effects. Antioxidative properties of the remaining extracts were noticeably weaker. SN - 1573-4919 UR - https://www.unboundmedicine.com/medline/citation/26350568/Antioxidant_action_of_six_Trifolium_species_in_blood_platelet_experimental_system_in_vitro_ L2 - https://doi.org/10.1007/s11010-015-2556-2 DB - PRIME DP - Unbound Medicine ER -