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In-depth study of Mollivirus sibericum, a new 30,000-y-old giant virus infecting Acanthamoeba.
Proc Natl Acad Sci U S A. 2015 Sep 22; 112(38):E5327-35.PN

Abstract

Acanthamoeba species are infected by the largest known DNA viruses. These include icosahedral Mimiviruses, amphora-shaped Pandoraviruses, and Pithovirus sibericum, the latter one isolated from 30,000-y-old permafrost. Mollivirus sibericum, a fourth type of giant virus, was isolated from the same permafrost sample. Its approximately spherical virion (0.6-µm diameter) encloses a 651-kb GC-rich genome encoding 523 proteins of which 64% are ORFans; 16% have their closest homolog in Pandoraviruses and 10% in Acanthamoeba castellanii probably through horizontal gene transfer. The Mollivirus nucleocytoplasmic replication cycle was analyzed using a combination of "omic" approaches that revealed how the virus highjacks its host machinery to actively replicate. Surprisingly, the host's ribosomal proteins are packaged in the virion. Metagenomic analysis of the permafrost sample uncovered the presence of both viruses, yet in very low amount. The fact that two different viruses retain their infectivity in prehistorical permafrost layers should be of concern in a context of global warming. Giant viruses' diversity remains to be fully explored.

Authors+Show Affiliations

Information Génomique and Structurale, Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée, FR3479) Centre National de la Recherche Scientifique, Aix-Marseille Université, 13288 Marseille Cedex 9, France;Information Génomique and Structurale, Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée, FR3479) Centre National de la Recherche Scientifique, Aix-Marseille Université, 13288 Marseille Cedex 9, France;Information Génomique and Structurale, Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée, FR3479) Centre National de la Recherche Scientifique, Aix-Marseille Université, 13288 Marseille Cedex 9, France;Information Génomique and Structurale, Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée, FR3479) Centre National de la Recherche Scientifique, Aix-Marseille Université, 13288 Marseille Cedex 9, France;Information Génomique and Structurale, Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée, FR3479) Centre National de la Recherche Scientifique, Aix-Marseille Université, 13288 Marseille Cedex 9, France;Information Génomique and Structurale, Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée, FR3479) Centre National de la Recherche Scientifique, Aix-Marseille Université, 13288 Marseille Cedex 9, France;Information Génomique and Structurale, Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée, FR3479) Centre National de la Recherche Scientifique, Aix-Marseille Université, 13288 Marseille Cedex 9, France;Université Grenoble Alpes, Institut de Recherches en Technologies et Sciences pour le Vivant-Laboratoire Biologie à Grande Echelle, F-38000 Grenoble, France; Commissariat à l'Energie Atomique, Centre National de la Recherche Scientifique, Institut de Recherches en Technologies et Sciences pour le Vivant-Laboratoire Biologie à Grande Echelle, F-38000 Grenoble, France; INSERM, Laboratoire Biologie à Grande Echelle, F-38000 Grenoble, France;Université Grenoble Alpes, Institut de Recherches en Technologies et Sciences pour le Vivant-Laboratoire Biologie à Grande Echelle, F-38000 Grenoble, France; Commissariat à l'Energie Atomique, Centre National de la Recherche Scientifique, Institut de Recherches en Technologies et Sciences pour le Vivant-Laboratoire Biologie à Grande Echelle, F-38000 Grenoble, France; INSERM, Laboratoire Biologie à Grande Echelle, F-38000 Grenoble, France;Commissariat à l'Energie Atomique, Institut de Génomique, Centre National de Séquençage, 91057 Evry Cedex, France;Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences, Pushchino 142290, Russia;Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences, Pushchino 142290, Russia;Université Grenoble Alpes, Institut de Recherches en Technologies et Sciences pour le Vivant-Laboratoire Biologie à Grande Echelle, F-38000 Grenoble, France; Commissariat à l'Energie Atomique, Centre National de la Recherche Scientifique, Institut de Recherches en Technologies et Sciences pour le Vivant-Laboratoire Biologie à Grande Echelle, F-38000 Grenoble, France; INSERM, Laboratoire Biologie à Grande Echelle, F-38000 Grenoble, France;Information Génomique and Structurale, Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée, FR3479) Centre National de la Recherche Scientifique, Aix-Marseille Université, 13288 Marseille Cedex 9, France; Chantal.Abergel@igs.cnrs-mrs.fr jean-michel.claverie@univ-amu.fr.Information Génomique and Structurale, Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée, FR3479) Centre National de la Recherche Scientifique, Aix-Marseille Université, 13288 Marseille Cedex 9, France; Assistance Publique-Hopitaux de Marseille, 13385 Marseille, France Chantal.Abergel@igs.cnrs-mrs.fr jean-michel.claverie@univ-amu.fr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26351664

Citation

Legendre, Matthieu, et al. "In-depth Study of Mollivirus Sibericum, a New 30,000-y-old Giant Virus Infecting Acanthamoeba." Proceedings of the National Academy of Sciences of the United States of America, vol. 112, no. 38, 2015, pp. E5327-35.
Legendre M, Lartigue A, Bertaux L, et al. In-depth study of Mollivirus sibericum, a new 30,000-y-old giant virus infecting Acanthamoeba. Proc Natl Acad Sci U S A. 2015;112(38):E5327-35.
Legendre, M., Lartigue, A., Bertaux, L., Jeudy, S., Bartoli, J., Lescot, M., Alempic, J. M., Ramus, C., Bruley, C., Labadie, K., Shmakova, L., Rivkina, E., Couté, Y., Abergel, C., & Claverie, J. M. (2015). In-depth study of Mollivirus sibericum, a new 30,000-y-old giant virus infecting Acanthamoeba. Proceedings of the National Academy of Sciences of the United States of America, 112(38), E5327-35. https://doi.org/10.1073/pnas.1510795112
Legendre M, et al. In-depth Study of Mollivirus Sibericum, a New 30,000-y-old Giant Virus Infecting Acanthamoeba. Proc Natl Acad Sci U S A. 2015 Sep 22;112(38):E5327-35. PubMed PMID: 26351664.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In-depth study of Mollivirus sibericum, a new 30,000-y-old giant virus infecting Acanthamoeba. AU - Legendre,Matthieu, AU - Lartigue,Audrey, AU - Bertaux,Lionel, AU - Jeudy,Sandra, AU - Bartoli,Julia, AU - Lescot,Magali, AU - Alempic,Jean-Marie, AU - Ramus,Claire, AU - Bruley,Christophe, AU - Labadie,Karine, AU - Shmakova,Lyubov, AU - Rivkina,Elizaveta, AU - Couté,Yohann, AU - Abergel,Chantal, AU - Claverie,Jean-Michel, Y1 - 2015/09/08/ PY - 2015/9/10/entrez PY - 2015/9/10/pubmed PY - 2016/1/2/medline KW - Pleistocene KW - giant virus KW - permafrost SP - E5327 EP - 35 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 112 IS - 38 N2 - Acanthamoeba species are infected by the largest known DNA viruses. These include icosahedral Mimiviruses, amphora-shaped Pandoraviruses, and Pithovirus sibericum, the latter one isolated from 30,000-y-old permafrost. Mollivirus sibericum, a fourth type of giant virus, was isolated from the same permafrost sample. Its approximately spherical virion (0.6-µm diameter) encloses a 651-kb GC-rich genome encoding 523 proteins of which 64% are ORFans; 16% have their closest homolog in Pandoraviruses and 10% in Acanthamoeba castellanii probably through horizontal gene transfer. The Mollivirus nucleocytoplasmic replication cycle was analyzed using a combination of "omic" approaches that revealed how the virus highjacks its host machinery to actively replicate. Surprisingly, the host's ribosomal proteins are packaged in the virion. Metagenomic analysis of the permafrost sample uncovered the presence of both viruses, yet in very low amount. The fact that two different viruses retain their infectivity in prehistorical permafrost layers should be of concern in a context of global warming. Giant viruses' diversity remains to be fully explored. SN - 1091-6490 UR - https://www.unboundmedicine.com/medline/citation/26351664/In_depth_study_of_Mollivirus_sibericum_a_new_30000_y_old_giant_virus_infecting_Acanthamoeba_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=26351664 DB - PRIME DP - Unbound Medicine ER -