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Sensitivity and Specificity of Histoplasma Antigen Detection by Enzyme Immunoassay.
J Am Anim Hosp Assoc. 2015 Sep-Oct; 51(5):306-10.JA

Abstract

The objective of this study was to evaluate the sensitivity and specificity of an antigen enzyme immunoassay (EIA) on urine samples for the diagnosis of histoplasmosis in dogs. This retrospective medical records review included canine cases with urine samples submitted for Histoplasma EIA antigen assay between 2007 and 2011 from three veterinary institutions. Cases for which urine samples were submitted for Histoplasma antigen testing were reviewed and compared to the gold standard of finding Histoplasma organisms or an alternative diagnosis on cytology or histopathology. Sensitivity, specificity, negative predictive value, positive predictive value, and the kappa coefficient and associated confidence interval were calculated for the EIA-based Histoplasma antigen assay. Sixty cases met the inclusion criteria. Seventeen cases were considered true positives based on identification of the organism, and 41 cases were considered true negatives with an alternative definitive diagnosis. Two cases were considered false negatives, and there were no false positives. Sensitivity was 89.47% and the negative predictive value was 95.35%. Specificity and the positive predictive value were both 100%. The kappa coefficient was 0.9207 (95% confidence interval, 0.8131-1). The Histoplasma antigen EIA test demonstrated high specificity and sensitivity for the diagnosis of histoplasmosis in dogs.

Authors+Show Affiliations

From the Department of Veterinary Clinical Sciences, Center for Veterinary Health Sciences (L.C., A.H.) and Department of Statistics (C.G.), Oklahoma State University, Stillwater, OK; Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX (A.C.); Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS (K.H.); and MiraVista Diagnostics, Indianapolis, IN (J.W., E.K.).From the Department of Veterinary Clinical Sciences, Center for Veterinary Health Sciences (L.C., A.H.) and Department of Statistics (C.G.), Oklahoma State University, Stillwater, OK; Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX (A.C.); Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS (K.H.); and MiraVista Diagnostics, Indianapolis, IN (J.W., E.K.).From the Department of Veterinary Clinical Sciences, Center for Veterinary Health Sciences (L.C., A.H.) and Department of Statistics (C.G.), Oklahoma State University, Stillwater, OK; Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX (A.C.); Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS (K.H.); and MiraVista Diagnostics, Indianapolis, IN (J.W., E.K.).From the Department of Veterinary Clinical Sciences, Center for Veterinary Health Sciences (L.C., A.H.) and Department of Statistics (C.G.), Oklahoma State University, Stillwater, OK; Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX (A.C.); Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS (K.H.); and MiraVista Diagnostics, Indianapolis, IN (J.W., E.K.).From the Department of Veterinary Clinical Sciences, Center for Veterinary Health Sciences (L.C., A.H.) and Department of Statistics (C.G.), Oklahoma State University, Stillwater, OK; Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX (A.C.); Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS (K.H.); and MiraVista Diagnostics, Indianapolis, IN (J.W., E.K.).From the Department of Veterinary Clinical Sciences, Center for Veterinary Health Sciences (L.C., A.H.) and Department of Statistics (C.G.), Oklahoma State University, Stillwater, OK; Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX (A.C.); Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS (K.H.); and MiraVista Diagnostics, Indianapolis, IN (J.W., E.K.).From the Department of Veterinary Clinical Sciences, Center for Veterinary Health Sciences (L.C., A.H.) and Department of Statistics (C.G.), Oklahoma State University, Stillwater, OK; Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX (A.C.); Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS (K.H.); and MiraVista Diagnostics, Indianapolis, IN (J.W., E.K.).

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26355580

Citation

Cunningham, Lauren, et al. "Sensitivity and Specificity of Histoplasma Antigen Detection By Enzyme Immunoassay." Journal of the American Animal Hospital Association, vol. 51, no. 5, 2015, pp. 306-10.
Cunningham L, Cook A, Hanzlicek A, et al. Sensitivity and Specificity of Histoplasma Antigen Detection by Enzyme Immunoassay. J Am Anim Hosp Assoc. 2015;51(5):306-10.
Cunningham, L., Cook, A., Hanzlicek, A., Harkin, K., Wheat, J., Goad, C., & Kirsch, E. (2015). Sensitivity and Specificity of Histoplasma Antigen Detection by Enzyme Immunoassay. Journal of the American Animal Hospital Association, 51(5), 306-10. https://doi.org/10.5326/JAAHA-MS-6202
Cunningham L, et al. Sensitivity and Specificity of Histoplasma Antigen Detection By Enzyme Immunoassay. J Am Anim Hosp Assoc. 2015 Sep-Oct;51(5):306-10. PubMed PMID: 26355580.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitivity and Specificity of Histoplasma Antigen Detection by Enzyme Immunoassay. AU - Cunningham,Lauren, AU - Cook,Audrey, AU - Hanzlicek,Andrew, AU - Harkin,Kenneth, AU - Wheat,Joseph, AU - Goad,Carla, AU - Kirsch,Emily, PY - 2015/9/11/entrez PY - 2015/9/12/pubmed PY - 2016/8/16/medline SP - 306 EP - 10 JF - Journal of the American Animal Hospital Association JO - J Am Anim Hosp Assoc VL - 51 IS - 5 N2 - The objective of this study was to evaluate the sensitivity and specificity of an antigen enzyme immunoassay (EIA) on urine samples for the diagnosis of histoplasmosis in dogs. This retrospective medical records review included canine cases with urine samples submitted for Histoplasma EIA antigen assay between 2007 and 2011 from three veterinary institutions. Cases for which urine samples were submitted for Histoplasma antigen testing were reviewed and compared to the gold standard of finding Histoplasma organisms or an alternative diagnosis on cytology or histopathology. Sensitivity, specificity, negative predictive value, positive predictive value, and the kappa coefficient and associated confidence interval were calculated for the EIA-based Histoplasma antigen assay. Sixty cases met the inclusion criteria. Seventeen cases were considered true positives based on identification of the organism, and 41 cases were considered true negatives with an alternative definitive diagnosis. Two cases were considered false negatives, and there were no false positives. Sensitivity was 89.47% and the negative predictive value was 95.35%. Specificity and the positive predictive value were both 100%. The kappa coefficient was 0.9207 (95% confidence interval, 0.8131-1). The Histoplasma antigen EIA test demonstrated high specificity and sensitivity for the diagnosis of histoplasmosis in dogs. SN - 0587-2871 UR - https://www.unboundmedicine.com/medline/citation/26355580/Sensitivity_and_Specificity_of_Histoplasma_Antigen_Detection_by_Enzyme_Immunoassay_ L2 - http://jaaha.org/doi/10.5326/JAAHA-MS-6202?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -