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Green tea extract activates AMPK and ameliorates white adipose tissue metabolic dysfunction induced by obesity.
Eur J Nutr. 2016 Oct; 55(7):2231-44.EJ

Abstract

PURPOSE

Beneficial effects of green tea (GT) polyphenols against obesity have been reported. However, until this moment the molecular mechanisms of how green tea can modulate obesity and regulates fat metabolism, particularly in adipose tissue, remain poorly understood. The aim of this study was to evaluate the role of GT extract in the adipose tissue of obese animals and its effect on weight gain, metabolism and function (de novo lipogenesis and lipolysis), and the involvement of AMP-activated protein kinase (AMPK).

METHODS AND RESULTS

Male Wistar rats were treated with GT by gavage (12 weeks/5 days/week; 500 mg/kg of body weight), and obesity was induced by cafeteria diet (8 weeks). Here, we show that obese rats treated with GT showed a significant reduction in indicators of obesity such as hyperlipidemia, fat synthesis, body weight, and fat depots as compared to those treated with standard control diet. AMPK was induced in adipose tissue in rats that were treated with GT and likely restored insulin sensitivity, increased mRNA expression of GLUT4, reducing the concentrations of plasma and liver lipid content, also stimulating fatty acid oxidation in the same tissue. Importantly, repression of de novo lipogenesis in the adipose tissue, reduced lipid droplets in the liver, and the development of insulin resistance in diet-induced obese rats were accompanied by AMPK activation.

CONCLUSION

Our study identified that metabolic changes caused by GT intake induced AMPK activation and modulate the expression of genes involved in metabolism, particularly in adipose tissue, thus offering a therapeutic strategy to combat insulin resistance, dyslipidemia, and obesity in rats.

Authors+Show Affiliations

Human Movement Sciences, Science Institute of Physical Activity and Sports, Cruzeiro do Sul University, Av. Regente Feijó, 1295, São Paulo, SP, 03342-000, Brazil.Human Movement Sciences, Science Institute of Physical Activity and Sports, Cruzeiro do Sul University, Av. Regente Feijó, 1295, São Paulo, SP, 03342-000, Brazil. Postgraduate Program of Pharmacology, Sao Paulo University, São Paulo, SP, Brazil.Mato Grosso do Sul University, Rodovia Ithaum, km 12 Cidade Universitária, Dourados, MS, 79804-970, Brazil.Human Movement Sciences, Science Institute of Physical Activity and Sports, Cruzeiro do Sul University, Av. Regente Feijó, 1295, São Paulo, SP, 03342-000, Brazil. rosemari.otton@cruzeirodosul.edu.br. Postgraduate Program of Pharmacology, Sao Paulo University, São Paulo, SP, Brazil. rosemari.otton@cruzeirodosul.edu.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26361764

Citation

Rocha, Andréa, et al. "Green Tea Extract Activates AMPK and Ameliorates White Adipose Tissue Metabolic Dysfunction Induced By Obesity." European Journal of Nutrition, vol. 55, no. 7, 2016, pp. 2231-44.
Rocha A, Bolin AP, Cardoso CA, et al. Green tea extract activates AMPK and ameliorates white adipose tissue metabolic dysfunction induced by obesity. Eur J Nutr. 2016;55(7):2231-44.
Rocha, A., Bolin, A. P., Cardoso, C. A., & Otton, R. (2016). Green tea extract activates AMPK and ameliorates white adipose tissue metabolic dysfunction induced by obesity. European Journal of Nutrition, 55(7), 2231-44. https://doi.org/10.1007/s00394-015-1033-8
Rocha A, et al. Green Tea Extract Activates AMPK and Ameliorates White Adipose Tissue Metabolic Dysfunction Induced By Obesity. Eur J Nutr. 2016;55(7):2231-44. PubMed PMID: 26361764.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Green tea extract activates AMPK and ameliorates white adipose tissue metabolic dysfunction induced by obesity. AU - Rocha,Andréa, AU - Bolin,Anaysa Paola, AU - Cardoso,Claudia Andrea Lima, AU - Otton,Rosemari, Y1 - 2015/09/11/ PY - 2015/01/07/received PY - 2015/09/01/accepted PY - 2015/9/13/entrez PY - 2015/9/13/pubmed PY - 2017/4/11/medline KW - Flavonoids KW - Gene expression KW - Metabolism KW - Obesity KW - Polyphenols SP - 2231 EP - 44 JF - European journal of nutrition JO - Eur J Nutr VL - 55 IS - 7 N2 - PURPOSE: Beneficial effects of green tea (GT) polyphenols against obesity have been reported. However, until this moment the molecular mechanisms of how green tea can modulate obesity and regulates fat metabolism, particularly in adipose tissue, remain poorly understood. The aim of this study was to evaluate the role of GT extract in the adipose tissue of obese animals and its effect on weight gain, metabolism and function (de novo lipogenesis and lipolysis), and the involvement of AMP-activated protein kinase (AMPK). METHODS AND RESULTS: Male Wistar rats were treated with GT by gavage (12 weeks/5 days/week; 500 mg/kg of body weight), and obesity was induced by cafeteria diet (8 weeks). Here, we show that obese rats treated with GT showed a significant reduction in indicators of obesity such as hyperlipidemia, fat synthesis, body weight, and fat depots as compared to those treated with standard control diet. AMPK was induced in adipose tissue in rats that were treated with GT and likely restored insulin sensitivity, increased mRNA expression of GLUT4, reducing the concentrations of plasma and liver lipid content, also stimulating fatty acid oxidation in the same tissue. Importantly, repression of de novo lipogenesis in the adipose tissue, reduced lipid droplets in the liver, and the development of insulin resistance in diet-induced obese rats were accompanied by AMPK activation. CONCLUSION: Our study identified that metabolic changes caused by GT intake induced AMPK activation and modulate the expression of genes involved in metabolism, particularly in adipose tissue, thus offering a therapeutic strategy to combat insulin resistance, dyslipidemia, and obesity in rats. SN - 1436-6215 UR - https://www.unboundmedicine.com/medline/citation/26361764/Green_tea_extract_activates_AMPK_and_ameliorates_white_adipose_tissue_metabolic_dysfunction_induced_by_obesity_ DB - PRIME DP - Unbound Medicine ER -