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Differential alterations of tissue T-cell subsets after sepsis.
Immunol Lett. 2015 Nov; 168(1):41-50.IL

Abstract

Among immune cells in responding to sepsis, macrophages and neutrophils have been extensively studied, while the contribution of T lymphocytes and natural killer T (NKT) cells is less well characterized. Here we monitored tissue specific changes of T cell subsets in male C57BL/6 mice subjected to sham operation or cecal ligation and puncture (CLP) to induce polymicrobial sepsis. Thymus, spleen, liver, lungs and blood were processed and analyzed 20h later. Total lymphocyte count showed a significant reduction in septic thymus, spleen and blood but not in lungs and liver. The septic thymi were hypocellular with severe reduction in cell numbers of immature CD4(+)CD8(+) subset. CD4(+) T and CD8(+) T lymphocyte numbers in septic spleens were also significantly reduced, but the frequency of CD4(+)CD25(+) Tregs was significantly increased. In addition, naïve and Tcm CD4(+) T cell numbers were significantly reduced in the septic spleens. By contrast, in septic liver the CD8(+) T cell numbers were significantly increased, whereas NKT cell numbers were reduced, but more activated with increased CD69 and CD25 expression. In the septic lungs, the CD4(+) T and CD8(+) T cell numbers showed no significant change, whereas they were severely reduced in the septic blood. Overall, this study provides important information on the alterations of different T-cell subsets in various tissues after sepsis.

Authors+Show Affiliations

Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, NY 11030, USA. Electronic address: asharma11@nshs.edu.Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, NY 11030, USA; Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, NY 11030, USA. Electronic address: wlyang@nshs.edu.Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, NY 11030, USA. Electronic address: Shingo.matsuo123@gmail.com.Center for Translational Research, The Feinstein Institute for Medical Research, Manhasset, NY 11030, USA; Department of Surgery, Hofstra North Shore-LIJ School of Medicine, Manhasset, NY 11030, USA. Electronic address: pwang@nshs.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

26362089

Citation

Sharma, Archna, et al. "Differential Alterations of Tissue T-cell Subsets After Sepsis." Immunology Letters, vol. 168, no. 1, 2015, pp. 41-50.
Sharma A, Yang WL, Matsuo S, et al. Differential alterations of tissue T-cell subsets after sepsis. Immunol Lett. 2015;168(1):41-50.
Sharma, A., Yang, W. L., Matsuo, S., & Wang, P. (2015). Differential alterations of tissue T-cell subsets after sepsis. Immunology Letters, 168(1), 41-50. https://doi.org/10.1016/j.imlet.2015.09.005
Sharma A, et al. Differential Alterations of Tissue T-cell Subsets After Sepsis. Immunol Lett. 2015;168(1):41-50. PubMed PMID: 26362089.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential alterations of tissue T-cell subsets after sepsis. AU - Sharma,Archna, AU - Yang,Weng-Lang, AU - Matsuo,Shingo, AU - Wang,Ping, Y1 - 2015/09/08/ PY - 2015/04/08/received PY - 2015/08/31/revised PY - 2015/09/03/accepted PY - 2015/9/13/entrez PY - 2015/9/13/pubmed PY - 2016/9/14/medline KW - Natural killer T cells KW - Profiling KW - Sepsis KW - T-lymphocytes KW - Tissue-specificity SP - 41 EP - 50 JF - Immunology letters JO - Immunol. Lett. VL - 168 IS - 1 N2 - Among immune cells in responding to sepsis, macrophages and neutrophils have been extensively studied, while the contribution of T lymphocytes and natural killer T (NKT) cells is less well characterized. Here we monitored tissue specific changes of T cell subsets in male C57BL/6 mice subjected to sham operation or cecal ligation and puncture (CLP) to induce polymicrobial sepsis. Thymus, spleen, liver, lungs and blood were processed and analyzed 20h later. Total lymphocyte count showed a significant reduction in septic thymus, spleen and blood but not in lungs and liver. The septic thymi were hypocellular with severe reduction in cell numbers of immature CD4(+)CD8(+) subset. CD4(+) T and CD8(+) T lymphocyte numbers in septic spleens were also significantly reduced, but the frequency of CD4(+)CD25(+) Tregs was significantly increased. In addition, naïve and Tcm CD4(+) T cell numbers were significantly reduced in the septic spleens. By contrast, in septic liver the CD8(+) T cell numbers were significantly increased, whereas NKT cell numbers were reduced, but more activated with increased CD69 and CD25 expression. In the septic lungs, the CD4(+) T and CD8(+) T cell numbers showed no significant change, whereas they were severely reduced in the septic blood. Overall, this study provides important information on the alterations of different T-cell subsets in various tissues after sepsis. SN - 1879-0542 UR - https://www.unboundmedicine.com/medline/citation/26362089/Differential_alterations_of_tissue_T_cell_subsets_after_sepsis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-2478(15)30029-8 DB - PRIME DP - Unbound Medicine ER -