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Determination of biogenic amines in beer and wine by capillary electrophoresis-tandem mass spectrometry.
J Chromatogr A. 2015 Oct 16; 1416:121-8.JC

Abstract

A capillary electrophoresis-tandem mass spectrometry (CE-MS/MS) method for the simultaneous assessment of nine biogenic amines (spermine, spermidine, putrescine, cadaverine, histamine, phenylethylamine, tryptamine, tyramine, and urocanic acid) in commercial samples of beer and wine is introduced. The samples were submitted to a simple clean-up step with poly(vinylpolypyrrolidone) followed by filtration. Electrophoretic separation in a polyvinyl alcohol (PVA)-coated capillary using 0.5 mol L(-1) acetic acid (pH 2.5) as background electrolyte and detection by electrospray-tandem mass spectrometry was employed. The range of the correlation coefficients of the calibration curves of the analyzed compounds was 0.996-0.999, and the limits of detection and limits of quantification were in the range of 1-2 μg L(-1) and 3-8 μg L(-1), respectively. The recovery values for samples spiked at three concentration levels (0.2, 0.5, and 1.0 mg L(-1)) ranged from 87 to 113% with standard deviation not greater than 5.8%. The use of a PVA-coated silica capillary allows suppressing the electroosmotic flow and, consequently, increasing of the separation efficiency. The method was successfully used to determine biogenic amines in commercial samples of beer and wine.

Authors+Show Affiliations

Agilent Technologies, Alameda Araguaia, 1142, CEP 06455-000 Barueri, SP, Brazil; Departamento de Química Fundamental - Instituto de Química - Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, CEP 05508-000 São Paulo, SP, Brazil. Electronic address: daniela_daniel@agilent.com.Departamento de Química Fundamental - Instituto de Química - Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, CEP 05508-000 São Paulo, SP, Brazil. Electronic address: vagnerbs@iq.usp.br.Departamento de Química Fundamental - Instituto de Química - Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, CEP 05508-000 São Paulo, SP, Brazil. Electronic address: denistadeu@gmail.com.Departamento de Química Fundamental - Instituto de Química - Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, CEP 05508-000 São Paulo, SP, Brazil. Electronic address: claudemi@iq.usp.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26362807

Citation

Daniel, Daniela, et al. "Determination of Biogenic Amines in Beer and Wine By Capillary Electrophoresis-tandem Mass Spectrometry." Journal of Chromatography. A, vol. 1416, 2015, pp. 121-8.
Daniel D, Dos Santos VB, Vidal DT, et al. Determination of biogenic amines in beer and wine by capillary electrophoresis-tandem mass spectrometry. J Chromatogr A. 2015;1416:121-8.
Daniel, D., Dos Santos, V. B., Vidal, D. T., & do Lago, C. L. (2015). Determination of biogenic amines in beer and wine by capillary electrophoresis-tandem mass spectrometry. Journal of Chromatography. A, 1416, 121-8. https://doi.org/10.1016/j.chroma.2015.08.065
Daniel D, et al. Determination of Biogenic Amines in Beer and Wine By Capillary Electrophoresis-tandem Mass Spectrometry. J Chromatogr A. 2015 Oct 16;1416:121-8. PubMed PMID: 26362807.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of biogenic amines in beer and wine by capillary electrophoresis-tandem mass spectrometry. AU - Daniel,Daniela, AU - Dos Santos,Vagner Bezerra, AU - Vidal,Denis Tadeu Rajh, AU - do Lago,Claudimir Lucio, Y1 - 2015/09/03/ PY - 2015/07/22/received PY - 2015/08/30/accepted PY - 2015/9/13/entrez PY - 2015/9/13/pubmed PY - 2016/5/4/medline KW - 2-Phenyl-ethylamine (PubChem CID 9075) KW - Beer KW - Biogenic amines KW - Cadaverine (PubChem CID 273) KW - Capillary electrophoresis KW - Histamine (PubChem CID 774) KW - Putrescine (PubChem CID 1045) KW - Spermidine (PubChem CID 1102) KW - Spermine (PubChem CID 1103) KW - Tandem mass spectrometry KW - Tryptamine (PubChem CID 1150) KW - Tyramine (PubChem CID 5610) KW - Urocanic acid (PubChem CID 736715) KW - Wine SP - 121 EP - 8 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1416 N2 - A capillary electrophoresis-tandem mass spectrometry (CE-MS/MS) method for the simultaneous assessment of nine biogenic amines (spermine, spermidine, putrescine, cadaverine, histamine, phenylethylamine, tryptamine, tyramine, and urocanic acid) in commercial samples of beer and wine is introduced. The samples were submitted to a simple clean-up step with poly(vinylpolypyrrolidone) followed by filtration. Electrophoretic separation in a polyvinyl alcohol (PVA)-coated capillary using 0.5 mol L(-1) acetic acid (pH 2.5) as background electrolyte and detection by electrospray-tandem mass spectrometry was employed. The range of the correlation coefficients of the calibration curves of the analyzed compounds was 0.996-0.999, and the limits of detection and limits of quantification were in the range of 1-2 μg L(-1) and 3-8 μg L(-1), respectively. The recovery values for samples spiked at three concentration levels (0.2, 0.5, and 1.0 mg L(-1)) ranged from 87 to 113% with standard deviation not greater than 5.8%. The use of a PVA-coated silica capillary allows suppressing the electroosmotic flow and, consequently, increasing of the separation efficiency. The method was successfully used to determine biogenic amines in commercial samples of beer and wine. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/26362807/Determination_of_biogenic_amines_in_beer_and_wine_by_capillary_electrophoresis_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(15)01262-5 DB - PRIME DP - Unbound Medicine ER -