Citation
Shi, Xiaojun, et al. "Evaluation of a Rapid Method for the Simultaneous Quantification of Ribavirin, Sofosbuvir and Its Metabolite in Rat Plasma By UPLC-MS/MS." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 1002, 2015, pp. 353-7.
Shi X, Zhu D, Lou J, et al. Evaluation of a rapid method for the simultaneous quantification of ribavirin, sofosbuvir and its metabolite in rat plasma by UPLC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2015;1002:353-7.
Shi, X., Zhu, D., Lou, J., Zhu, B., Hu, A. R., & Gan, D. (2015). Evaluation of a rapid method for the simultaneous quantification of ribavirin, sofosbuvir and its metabolite in rat plasma by UPLC-MS/MS. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 1002, 353-7. https://doi.org/10.1016/j.jchromb.2015.08.038
Shi X, et al. Evaluation of a Rapid Method for the Simultaneous Quantification of Ribavirin, Sofosbuvir and Its Metabolite in Rat Plasma By UPLC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Oct 1;1002:353-7. PubMed PMID: 26363369.
TY - JOUR
T1 - Evaluation of a rapid method for the simultaneous quantification of ribavirin, sofosbuvir and its metabolite in rat plasma by UPLC-MS/MS.
AU - Shi,Xiaojun,
AU - Zhu,Dedong,
AU - Lou,Jie,
AU - Zhu,Bo,
AU - Hu,Ai-rong,
AU - Gan,Dongmei,
Y1 - 2015/08/30/
PY - 2015/07/09/received
PY - 2015/08/23/revised
PY - 2015/08/25/accepted
PY - 2015/9/13/entrez
PY - 2015/9/13/pubmed
PY - 2016/5/18/medline
KW - GS-331007
KW - Pharmacokinetics
KW - Plasma
KW - Ribavirin
KW - Sofosbuvir
KW - UPLC–MS/MS
SP - 353
EP - 7
JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
JO - J Chromatogr B Analyt Technol Biomed Life Sci
VL - 1002
N2 - A rapid and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the determination of ribavirin, sofosbuvir and its metabolite GS-331007 in rat plasma was established. The analytes and the internal standard (midazolam) were separated on an Acquity UPLC BEH C18 chromatography column (2.1mm×50mm, 1.7μm) using gradient elution with a mobile phase of acetonitrile and 0.1% formic acid in water at a flow rate of 0.4mL/min. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode to monitor the precursor-to-product ion transitions of m/z 245.1→113.1 for ribavirin, m/z 530.3→243.1 for sofosbuvir, m/z 261.5→113.1 for GS-331007 and m/z 326.2→291.1 for midazolam (IS) using a positive electrospray ionization interface. The method was validated over a concentration range of 5-1000ng/mL for ribavirin, 10-2000ng/mL for sofosbuvir and 10-2000ng/mL for GS-331007. Total time for each chromatograph was 3.0min. The intra- and inter-day precision and accuracy of the quality control samples at low, medium, and high concentration levels exhibited relative standard deviations (RSD) <10.0% and the accuracy values ranged from -10.6% to 11.6%. The method was successfully applied to a pharmacokinetic study of ribavirin, sofosbuvir and GS-331007 in rats.
SN - 1873-376X
UR - https://www.unboundmedicine.com/medline/citation/26363369/Evaluation_of_a_rapid_method_for_the_simultaneous_quantification_of_ribavirin_sofosbuvir_and_its_metabolite_in_rat_plasma_by_UPLC_MS/MS_
DB - PRIME
DP - Unbound Medicine
ER -
