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Evidence of gastric ulcer healing activity of Maytenus robusta Reissek: In vitro and in vivo studies.
J Ethnopharmacol. 2015 Dec 04; 175:75-85.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Maytenus robusta Reissek (Celastraceae) is traditionally used in Brazilian folk medicine to treat gastric ulcer, as a substitute for M. ilicifolia, which is almost extinct. The gastroprotective properties of M. robusta were demonstrated previously using only preventive approaches, such as acute gastric ulcer models. However, the healing effect of M. robusta in gastric ulcers remains unclear.

AIM OF THE STUDY

The current study was carried out to investigate the healing effectiveness of M. robusta hydroalcoholic extract (HEMR) from aerial parts in the acetic acid-induced chronic ulcer model and to determine its effect on cell proliferation, scavenging free radicals, and inflammatory and oxidative damage.

MATERIAL AND METHODS

To evaluate the healing properties of HEMR in vivo, chronic gastric ulcer was induced in rats by 80% acid acetic. Next, different groups of animals (n=6) were treated orally with vehicle (water plus 1% tween, 1 ml/kg), omeprazole (20mg/kg), or HEMR (1-10mg/kg), twice daily for 7 days. At the end of the treatment, the total ulcer area (mm(2)) was measured and a sample of gastric tissue was taken for histological and histochemical analysis. Evaluation of GSH and LOOH levels, GST, SOD, CAT and MPO activity was also performed at the site of the lesion. In parallel, radical scavenging activity, cytoprotective effect, and cell proliferation activity in fibroblasts (L929 cells) were determined by in vitro trials. The antisecretory properties were evaluated using the pylorus ligature model in rats, and the anti-Helicobacter pylori activity was determined in vitro. Acute toxicity was evaluated by relative organ weight and biochemical parameters in serum. The prokinetic properties were also evaluated in mice.

RESULTS

Oral administration of HEMR (10mg/kg) reduced the gastric ulcer area by 53%, compared to the vehicle group (120.0 ± 8.3mm(2)), the regeneration of gastric mucosa was evidenced in histological analysis. Moreover, HEMR treatment increased gastric mucin content and reduced oxidative stress and inflammatory parameters at the site of the ulcer. In vitro, HEMR (1-1000 µg/ml) was able to scavenge free radical DPPH and promote cytoprotection against H2O2 in fibroblasts at 0.1-100 µg/ml. Moreover, HEMR healing properties also were confirmed by enhancement of proliferation and coverage of scratched wounds in fibroblast monolayer. However, HEMR (10mg/kg) by the intraduodenal route did not promote changes in volume, pH, total acidity or pepsin activity in the pylorus ligature model, and HEMR up to 2000 µg/ml also did not present considerable activity against H. pylori. In relation to gastrointestinal motility, HEMR (10mg/kg, p.o) did not provoke alterations. It is also important to mention that oral administration of HEMR did not produce any sign of acute toxicity in animals.

CONCLUSIONS

The data here obtained show that M. robusta has evident ulcer healing potential, mainly through the strengthening of protective factors of gastric mucosa, such as mucus layer, antioxidant defenses and cell proliferation. Taking into account the advantages of cultivation and harvesting of M. robusta compared to M. ilicifolia, and the evidence presented here, it is plausible to conclude that hydroalcoholic extract obtained from aerial parts of M. robusta is an interesting source for the development of a phytotherapeutic formulation to treat gastric ulcer.

Authors+Show Affiliations

Programa de Pós-Graduação em Ciência s Farmacêuticas (PPGCF), Universidade do Vale do Itajaí (UNIVALI), Itajaí, SC, Brazil. Electronic address: lu.isamota@hotmail.com.Programa de Pós-Graduação em Ciência s Farmacêuticas (PPGCF), Universidade do Vale do Itajaí (UNIVALI), Itajaí, SC, Brazil.Programa de Pós-Graduação em Ciência s Farmacêuticas (PPGCF), Universidade do Vale do Itajaí (UNIVALI), Itajaí, SC, Brazil.Programa de Pós-Graduação em Ciência s Farmacêuticas (PPGCF), Universidade do Vale do Itajaí (UNIVALI), Itajaí, SC, Brazil.Programa de Pós-Graduação em Ciência s Farmacêuticas (PPGCF), Universidade do Vale do Itajaí (UNIVALI), Itajaí, SC, Brazil.Departamento de Ciências Farmacêuticas, Universidade Regional de Blumenau (FURB), Blumenau, SC, Brazil.Programa de Pós-Graduação em Ciência s Farmacêuticas (PPGCF), Universidade do Vale do Itajaí (UNIVALI), Itajaí, SC, Brazil.Programa de Pós-Graduação em Ciência s Farmacêuticas (PPGCF), Universidade do Vale do Itajaí (UNIVALI), Itajaí, SC, Brazil.Programa de Pós-Graduação em Ciência s Farmacêuticas (PPGCF), Universidade do Vale do Itajaí (UNIVALI), Itajaí, SC, Brazil.Programa de Pós-Graduação em Ciência s Farmacêuticas (PPGCF), Universidade do Vale do Itajaí (UNIVALI), Itajaí, SC, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26364940

Citation

da Silva, Luisa Mota, et al. "Evidence of Gastric Ulcer Healing Activity of Maytenus Robusta Reissek: in Vitro and in Vivo Studies." Journal of Ethnopharmacology, vol. 175, 2015, pp. 75-85.
da Silva LM, Boeing T, Somensi LB, et al. Evidence of gastric ulcer healing activity of Maytenus robusta Reissek: In vitro and in vivo studies. J Ethnopharmacol. 2015;175:75-85.
da Silva, L. M., Boeing, T., Somensi, L. B., Cury, B. J., Steimbach, V. M., Silveria, A. C., Niero, R., Cechinel Filho, V., Santin, J. R., & de Andrade, S. F. (2015). Evidence of gastric ulcer healing activity of Maytenus robusta Reissek: In vitro and in vivo studies. Journal of Ethnopharmacology, 175, 75-85. https://doi.org/10.1016/j.jep.2015.09.006
da Silva LM, et al. Evidence of Gastric Ulcer Healing Activity of Maytenus Robusta Reissek: in Vitro and in Vivo Studies. J Ethnopharmacol. 2015 Dec 4;175:75-85. PubMed PMID: 26364940.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence of gastric ulcer healing activity of Maytenus robusta Reissek: In vitro and in vivo studies. AU - da Silva,Luisa Mota, AU - Boeing,Thaise, AU - Somensi,Lincon Bordignon, AU - Cury,Benhur Judah, AU - Steimbach,Viviane Miranda Bispo, AU - Silveria,Alessandro Conrado de Oliveira, AU - Niero,Rivaldo, AU - Cechinel Filho,Valdir, AU - Santin,José Roberto, AU - de Andrade,Sérgio Faloni, Y1 - 2015/09/10/ PY - 2015/07/08/received PY - 2015/08/31/revised PY - 2015/09/05/accepted PY - 2015/9/15/entrez PY - 2015/9/15/pubmed PY - 2016/10/7/medline KW - Antioxidant KW - Antiulcer KW - Fibroblast KW - Gastroprotection KW - Healing ulcer KW - M. robusta SP - 75 EP - 85 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 175 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Maytenus robusta Reissek (Celastraceae) is traditionally used in Brazilian folk medicine to treat gastric ulcer, as a substitute for M. ilicifolia, which is almost extinct. The gastroprotective properties of M. robusta were demonstrated previously using only preventive approaches, such as acute gastric ulcer models. However, the healing effect of M. robusta in gastric ulcers remains unclear. AIM OF THE STUDY: The current study was carried out to investigate the healing effectiveness of M. robusta hydroalcoholic extract (HEMR) from aerial parts in the acetic acid-induced chronic ulcer model and to determine its effect on cell proliferation, scavenging free radicals, and inflammatory and oxidative damage. MATERIAL AND METHODS: To evaluate the healing properties of HEMR in vivo, chronic gastric ulcer was induced in rats by 80% acid acetic. Next, different groups of animals (n=6) were treated orally with vehicle (water plus 1% tween, 1 ml/kg), omeprazole (20mg/kg), or HEMR (1-10mg/kg), twice daily for 7 days. At the end of the treatment, the total ulcer area (mm(2)) was measured and a sample of gastric tissue was taken for histological and histochemical analysis. Evaluation of GSH and LOOH levels, GST, SOD, CAT and MPO activity was also performed at the site of the lesion. In parallel, radical scavenging activity, cytoprotective effect, and cell proliferation activity in fibroblasts (L929 cells) were determined by in vitro trials. The antisecretory properties were evaluated using the pylorus ligature model in rats, and the anti-Helicobacter pylori activity was determined in vitro. Acute toxicity was evaluated by relative organ weight and biochemical parameters in serum. The prokinetic properties were also evaluated in mice. RESULTS: Oral administration of HEMR (10mg/kg) reduced the gastric ulcer area by 53%, compared to the vehicle group (120.0 ± 8.3mm(2)), the regeneration of gastric mucosa was evidenced in histological analysis. Moreover, HEMR treatment increased gastric mucin content and reduced oxidative stress and inflammatory parameters at the site of the ulcer. In vitro, HEMR (1-1000 µg/ml) was able to scavenge free radical DPPH and promote cytoprotection against H2O2 in fibroblasts at 0.1-100 µg/ml. Moreover, HEMR healing properties also were confirmed by enhancement of proliferation and coverage of scratched wounds in fibroblast monolayer. However, HEMR (10mg/kg) by the intraduodenal route did not promote changes in volume, pH, total acidity or pepsin activity in the pylorus ligature model, and HEMR up to 2000 µg/ml also did not present considerable activity against H. pylori. In relation to gastrointestinal motility, HEMR (10mg/kg, p.o) did not provoke alterations. It is also important to mention that oral administration of HEMR did not produce any sign of acute toxicity in animals. CONCLUSIONS: The data here obtained show that M. robusta has evident ulcer healing potential, mainly through the strengthening of protective factors of gastric mucosa, such as mucus layer, antioxidant defenses and cell proliferation. Taking into account the advantages of cultivation and harvesting of M. robusta compared to M. ilicifolia, and the evidence presented here, it is plausible to conclude that hydroalcoholic extract obtained from aerial parts of M. robusta is an interesting source for the development of a phytotherapeutic formulation to treat gastric ulcer. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/26364940/Evidence_of_gastric_ulcer_healing_activity_of_Maytenus_robusta_Reissek:_In_vitro_and_in_vivo_studies_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(15)30131-8 DB - PRIME DP - Unbound Medicine ER -