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Structure and function of the amygdaloid NPY system: NPY Y2 receptors regulate excitatory and inhibitory synaptic transmission in the centromedial amygdala.
Brain Struct Funct 2016; 221(7):3373-91BS

Abstract

The amygdala is essential for generating emotional-affective behaviors. It consists of several nuclei with highly selective, elaborate functions. In particular, the central extended amygdala, consisting of the central amygdala (CEA) and the bed nucleus of the stria terminalis (BNST) is an essential component actively controlling efferent connections to downstream effectors like hypothalamus and brain stem. Both, CEA and BNST contain high amounts of different neuropeptides that significantly contribute to synaptic transmission. Among these, neuropeptide Y (NPY) has emerged as an important anxiolytic and fear-reducing neuromodulator. Here, we characterized the expression, connectivity and electrophysiological function of NPY and Y2 receptors within the CEA. We identified several NPY-expressing neuronal populations, including somatostatin- and calretinin-expressing neurons. Furthermore, in the main intercalated nucleus, NPY is expressed primarily in dopamine D1 receptor-expressing neurons but also in interspersed somatostatin-expressing neurons. Interestingly, NPY neurons did not co-localize with the Y2 receptor. Retrograde tract tracing experiments revealed that NPY neurons reciprocally connect the CEA and BNST. Functionally, the Y2 receptor agonist PYY3-36, reduced both, inhibitory as well as excitatory synaptic transmission in the centromedial amygdala (CEm). However, we also provide evidence that lack of NPY or Y2 receptors results in increased GABA release specifically at inhibitory synapses in the CEm. Taken together, our findings suggest that NPY expressed by distinct populations of neurons can modulate afferent and efferent projections of the CEA via presynaptic Y2 receptors located at inhibitory and excitatory synapses.

Authors+Show Affiliations

Department of Pharmacology, Medical University Innsbruck, Peter-Mayr-Strasse 1a, 6020, Innsbruck, Austria.Department of Pharmacology, Medical University Innsbruck, Peter-Mayr-Strasse 1a, 6020, Innsbruck, Austria. Institute of Physiology I (Neurophysiology), Westfälische Wilhelms-Universität, Munster, Germany.Department of Pharmacology, Medical University Innsbruck, Peter-Mayr-Strasse 1a, 6020, Innsbruck, Austria. Capes Foundation, Ministry of Education of Brazil, Brasília, DF, 70040-020, Brazil.Janssen Research & Development, LLC, San Diego, CA, USA.Neuroscience Division, Garvan Institute of Medical Research, Darlinghurst, Sydney, NSW, 2010, Australia.Department of Pharmacology, Medical University Innsbruck, Peter-Mayr-Strasse 1a, 6020, Innsbruck, Austria.Department of Pharmacology, Medical University Innsbruck, Peter-Mayr-Strasse 1a, 6020, Innsbruck, Austria. ramon.tasan@i-med.ac.at.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26365505

Citation

Wood, J, et al. "Structure and Function of the Amygdaloid NPY System: NPY Y2 Receptors Regulate Excitatory and Inhibitory Synaptic Transmission in the Centromedial Amygdala." Brain Structure & Function, vol. 221, no. 7, 2016, pp. 3373-91.
Wood J, Verma D, Lach G, et al. Structure and function of the amygdaloid NPY system: NPY Y2 receptors regulate excitatory and inhibitory synaptic transmission in the centromedial amygdala. Brain Struct Funct. 2016;221(7):3373-91.
Wood, J., Verma, D., Lach, G., Bonaventure, P., Herzog, H., Sperk, G., & Tasan, R. O. (2016). Structure and function of the amygdaloid NPY system: NPY Y2 receptors regulate excitatory and inhibitory synaptic transmission in the centromedial amygdala. Brain Structure & Function, 221(7), pp. 3373-91. doi:10.1007/s00429-015-1107-7.
Wood J, et al. Structure and Function of the Amygdaloid NPY System: NPY Y2 Receptors Regulate Excitatory and Inhibitory Synaptic Transmission in the Centromedial Amygdala. Brain Struct Funct. 2016;221(7):3373-91. PubMed PMID: 26365505.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structure and function of the amygdaloid NPY system: NPY Y2 receptors regulate excitatory and inhibitory synaptic transmission in the centromedial amygdala. AU - Wood,J, AU - Verma,D, AU - Lach,G, AU - Bonaventure,P, AU - Herzog,H, AU - Sperk,G, AU - Tasan,R O, Y1 - 2015/09/13/ PY - 2015/03/24/received PY - 2015/09/04/accepted PY - 2015/9/15/entrez PY - 2015/9/15/pubmed PY - 2017/9/30/medline KW - Central amygdala KW - Dopamine D1 receptor KW - Intercalated neurons KW - NPY KW - Neuropeptide Y KW - Y2 receptor SP - 3373 EP - 91 JF - Brain structure & function JO - Brain Struct Funct VL - 221 IS - 7 N2 - The amygdala is essential for generating emotional-affective behaviors. It consists of several nuclei with highly selective, elaborate functions. In particular, the central extended amygdala, consisting of the central amygdala (CEA) and the bed nucleus of the stria terminalis (BNST) is an essential component actively controlling efferent connections to downstream effectors like hypothalamus and brain stem. Both, CEA and BNST contain high amounts of different neuropeptides that significantly contribute to synaptic transmission. Among these, neuropeptide Y (NPY) has emerged as an important anxiolytic and fear-reducing neuromodulator. Here, we characterized the expression, connectivity and electrophysiological function of NPY and Y2 receptors within the CEA. We identified several NPY-expressing neuronal populations, including somatostatin- and calretinin-expressing neurons. Furthermore, in the main intercalated nucleus, NPY is expressed primarily in dopamine D1 receptor-expressing neurons but also in interspersed somatostatin-expressing neurons. Interestingly, NPY neurons did not co-localize with the Y2 receptor. Retrograde tract tracing experiments revealed that NPY neurons reciprocally connect the CEA and BNST. Functionally, the Y2 receptor agonist PYY3-36, reduced both, inhibitory as well as excitatory synaptic transmission in the centromedial amygdala (CEm). However, we also provide evidence that lack of NPY or Y2 receptors results in increased GABA release specifically at inhibitory synapses in the CEm. Taken together, our findings suggest that NPY expressed by distinct populations of neurons can modulate afferent and efferent projections of the CEA via presynaptic Y2 receptors located at inhibitory and excitatory synapses. SN - 1863-2661 UR - https://www.unboundmedicine.com/medline/citation/26365505/Structure_and_function_of_the_amygdaloid_NPY_system:_NPY_Y2_receptors_regulate_excitatory_and_inhibitory_synaptic_transmission_in_the_centromedial_amygdala_ L2 - https://dx.doi.org/10.1007/s00429-015-1107-7 DB - PRIME DP - Unbound Medicine ER -