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Preparation and validation of implantable electrodes for the measurement of oxygen and glucose.
Biomed Biochim Acta. 1989; 48(11-12):911-7.BB

Abstract

In realizing the continuous measurement of pO2 and glucose concentration in the subcutaneous tissue, miniaturized electrochemical oxygen- and enzyme glucose sensors, based on modified Clark-type electrodes for transient implantation, were developed and investigated. The electrodes were prepared by means of sequential dipping procedures in glucose oxidase and in different polymer solutions at well-defined environmental conditions in an incubator. By means of combining a hydrophobic membrane with a glucose permeable area and a hydrophilic membrane in the case of the glucose sensor, linearity of the glucose dependent electrode signal up to greater than 20 mmol/l could be achieved. After subcutaneous implantation in the neck of dogs, the enzyme sensor is able to follow glucose profiles in the normo- and in the hyperglycaemic range, e.g. as caused by oral glucose loads. Looking for the difference in sensitivity of the enzyme sensor measured in vitro and calculated from in vivo data, the influence of potential nonspecific, interfering substances in vivo such as urea, amino acids, electrolytes, and albumin was estimated.

Authors+Show Affiliations

Central Institute of Diabetes, Gerhardt Katsch, Karlsburg, GDR.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2636836

Citation

Brunstein, E, et al. "Preparation and Validation of Implantable Electrodes for the Measurement of Oxygen and Glucose." Biomedica Biochimica Acta, vol. 48, no. 11-12, 1989, pp. 911-7.
Brunstein E, Abel P, Gens A, et al. Preparation and validation of implantable electrodes for the measurement of oxygen and glucose. Biomed Biochim Acta. 1989;48(11-12):911-7.
Brunstein, E., Abel, P., Gens, A., Eich, K., & Woedtke, T. V. (1989). Preparation and validation of implantable electrodes for the measurement of oxygen and glucose. Biomedica Biochimica Acta, 48(11-12), 911-7.
Brunstein E, et al. Preparation and Validation of Implantable Electrodes for the Measurement of Oxygen and Glucose. Biomed Biochim Acta. 1989;48(11-12):911-7. PubMed PMID: 2636836.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation and validation of implantable electrodes for the measurement of oxygen and glucose. AU - Brunstein,E, AU - Abel,P, AU - Gens,A, AU - Eich,K, AU - Woedtke,T V, PY - 1989/1/1/pubmed PY - 1989/1/1/medline PY - 1989/1/1/entrez SP - 911 EP - 7 JF - Biomedica biochimica acta JO - Biomed Biochim Acta VL - 48 IS - 11-12 N2 - In realizing the continuous measurement of pO2 and glucose concentration in the subcutaneous tissue, miniaturized electrochemical oxygen- and enzyme glucose sensors, based on modified Clark-type electrodes for transient implantation, were developed and investigated. The electrodes were prepared by means of sequential dipping procedures in glucose oxidase and in different polymer solutions at well-defined environmental conditions in an incubator. By means of combining a hydrophobic membrane with a glucose permeable area and a hydrophilic membrane in the case of the glucose sensor, linearity of the glucose dependent electrode signal up to greater than 20 mmol/l could be achieved. After subcutaneous implantation in the neck of dogs, the enzyme sensor is able to follow glucose profiles in the normo- and in the hyperglycaemic range, e.g. as caused by oral glucose loads. Looking for the difference in sensitivity of the enzyme sensor measured in vitro and calculated from in vivo data, the influence of potential nonspecific, interfering substances in vivo such as urea, amino acids, electrolytes, and albumin was estimated. SN - 0232-766X UR - https://www.unboundmedicine.com/medline/citation/2636836/Preparation_and_validation_of_implantable_electrodes_for_the_measurement_of_oxygen_and_glucose_ L2 - https://medlineplus.gov/bloodsugar.html DB - PRIME DP - Unbound Medicine ER -