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Molecular Characterization and In Vitro Antifungal Susceptibility of 316 Clinical Isolates of Dermatophytes in Iran.
Mycopathologia. 2016 Feb; 181(1-2):89-95.M

Abstract

Dermatophytosis is a common mycotic infection of the skin, nail, and hair, associated with major public health concern worldwide. Various species of dermatophytes show significant differences in susceptibility to antifungals. Here, we present the antifungal susceptibility of a large collection of molecularly identified dermatophyte isolates obtained from tropical region of south of Iran. A total of 9485 patients clinically suspected to have cutaneous fungal infections were examined. Dermatophytosis was confirmed in 1502 cases by direct microscopy and culture. Three hundred and sixteen isolates recovered in culture were identified to species level using PCR sequencing of ITS region and RFLP. Tinea corporis was the most prevalent type of clinical manifestation (35.2 %), followed by tinea cruris (17 %), tinea capitis (12.8 %), tinea pedis (11.3 %), tinea manuum (11 %), tinea unguium (6.9 %), and tinea barbae (5.8 %). Trichophyton interdigitale was the most common isolate (49.36 %), followed by Trichophyton rubrum (18.98 %), Epidermophyton floccosum (13.29 %), Microsporum canis (9.17 %), Arthroderma benhamiae (T. anamorph of A. benhamiae; 5.38 %), and Trichophyton tonsurans (3.79 %). Overall, irrespective of the geographical region, terbinafine was the most potent antifungal against all isolates, with an MIC range of 0.002-0.25 μg/mL, followed by itraconazole (0.004-0.5 μg/mL), griseofulvin (0.125-8 μg/mL), and fluconazole (4-128 μg/mL). Analysis of our data revealed a significant increase in the frequency of A.benhamiae, which definitely warrants further investigation to explore source of this infection in south of Iran. Moreover, terbinafine was the most effective antifungal against all isolates, in vitro.

Authors+Show Affiliations

Invasive Fungi Research Center, Mazandaran University of Medical Sciences, Km 18 Khazarabad Road, P.O. Box 48175-1665, Sari, Iran. Department of Medical Mycology and Parasitology School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.Invasive Fungi Research Center, Mazandaran University of Medical Sciences, Km 18 Khazarabad Road, P.O. Box 48175-1665, Sari, Iran. hedayatimt@gmail.com. Department of Medical Mycology and Parasitology School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. hedayatimt@gmail.com.Department of Parasitology and Mycology, Basic Sciences in Infectious Diseases Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Department of Parasitology and Mycology, Basic Sciences in Infectious Diseases Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Invasive Fungi Research Center, Mazandaran University of Medical Sciences, Km 18 Khazarabad Road, P.O. Box 48175-1665, Sari, Iran. Department of Medical Mycology and Parasitology School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.Department of Parasitology and Mycology/Infectious and Tropical Medicine Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Department of Parasitology and Mycology, Basic Sciences in Infectious Diseases Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Invasive Fungi Research Center, Mazandaran University of Medical Sciences, Km 18 Khazarabad Road, P.O. Box 48175-1665, Sari, Iran. Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center, Erasmus, The Netherlands. Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26369643

Citation

Ansari, Saham, et al. "Molecular Characterization and in Vitro Antifungal Susceptibility of 316 Clinical Isolates of Dermatophytes in Iran." Mycopathologia, vol. 181, no. 1-2, 2016, pp. 89-95.
Ansari S, Hedayati MT, Zomorodian K, et al. Molecular Characterization and In Vitro Antifungal Susceptibility of 316 Clinical Isolates of Dermatophytes in Iran. Mycopathologia. 2016;181(1-2):89-95.
Ansari, S., Hedayati, M. T., Zomorodian, K., Pakshir, K., Badali, H., Rafiei, A., Ravandeh, M., & Seyedmousavi, S. (2016). Molecular Characterization and In Vitro Antifungal Susceptibility of 316 Clinical Isolates of Dermatophytes in Iran. Mycopathologia, 181(1-2), 89-95. https://doi.org/10.1007/s11046-015-9941-y
Ansari S, et al. Molecular Characterization and in Vitro Antifungal Susceptibility of 316 Clinical Isolates of Dermatophytes in Iran. Mycopathologia. 2016;181(1-2):89-95. PubMed PMID: 26369643.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular Characterization and In Vitro Antifungal Susceptibility of 316 Clinical Isolates of Dermatophytes in Iran. AU - Ansari,Saham, AU - Hedayati,Mohammad T, AU - Zomorodian,Kamiar, AU - Pakshir,Keyvan, AU - Badali,Hamid, AU - Rafiei,Abdollah, AU - Ravandeh,Mostafa, AU - Seyedmousavi,Seyedmojtaba, Y1 - 2015/09/14/ PY - 2015/05/15/received PY - 2015/09/05/accepted PY - 2015/9/16/entrez PY - 2015/9/16/pubmed PY - 2016/10/7/medline KW - Antifungal susceptibility KW - Arthroderma benhamiae KW - Dermatophytosis KW - Molecular identification KW - South of Iran SP - 89 EP - 95 JF - Mycopathologia JO - Mycopathologia VL - 181 IS - 1-2 N2 - Dermatophytosis is a common mycotic infection of the skin, nail, and hair, associated with major public health concern worldwide. Various species of dermatophytes show significant differences in susceptibility to antifungals. Here, we present the antifungal susceptibility of a large collection of molecularly identified dermatophyte isolates obtained from tropical region of south of Iran. A total of 9485 patients clinically suspected to have cutaneous fungal infections were examined. Dermatophytosis was confirmed in 1502 cases by direct microscopy and culture. Three hundred and sixteen isolates recovered in culture were identified to species level using PCR sequencing of ITS region and RFLP. Tinea corporis was the most prevalent type of clinical manifestation (35.2 %), followed by tinea cruris (17 %), tinea capitis (12.8 %), tinea pedis (11.3 %), tinea manuum (11 %), tinea unguium (6.9 %), and tinea barbae (5.8 %). Trichophyton interdigitale was the most common isolate (49.36 %), followed by Trichophyton rubrum (18.98 %), Epidermophyton floccosum (13.29 %), Microsporum canis (9.17 %), Arthroderma benhamiae (T. anamorph of A. benhamiae; 5.38 %), and Trichophyton tonsurans (3.79 %). Overall, irrespective of the geographical region, terbinafine was the most potent antifungal against all isolates, with an MIC range of 0.002-0.25 μg/mL, followed by itraconazole (0.004-0.5 μg/mL), griseofulvin (0.125-8 μg/mL), and fluconazole (4-128 μg/mL). Analysis of our data revealed a significant increase in the frequency of A.benhamiae, which definitely warrants further investigation to explore source of this infection in south of Iran. Moreover, terbinafine was the most effective antifungal against all isolates, in vitro. SN - 1573-0832 UR - https://www.unboundmedicine.com/medline/citation/26369643/Molecular_Characterization_and_In_Vitro_Antifungal_Susceptibility_of_316_Clinical_Isolates_of_Dermatophytes_in_Iran_ L2 - https://dx.doi.org/10.1007/s11046-015-9941-y DB - PRIME DP - Unbound Medicine ER -