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RNAi knockdown of fatty acid elongase1 alters fatty acid composition in Brassica napus.
Biochem Biophys Res Commun. 2015 Oct 23; 466(3):518-22.BB

Abstract

The quality and end-use of oil from oilseed crops is determined by its fatty acid composition. In particular, the relative proportions of erucic and oleic acids are key selection traits for breeders. The goal of our research is to genetically improve the nutritional quality of Brassica napus cultivar CY2, the oil of which is high in erucic acid (about 40%) and low in oleic acid (about 20%). Here, we report the use of a seed-specific napin A promoter to drive the knockdown of BnFAE1 in transgenic CY2. Southern blotting results confirmed the presence of the transgene. RT-PCR analysis showed that the levels of BnFAE1 were greatly decreased in BnFAE1-Ri lines compared with the CY2 cultivar. Knockdown of BnFAE1 sharply decreased the levels of erucic acid (less than 3%), largely increased the contents of oleic acid (more than 60%) and slightly increased the polyunsaturated chain fatty acids. Compared with high erucic acid parents, expression of BnFAE1 was dramatically decreased in developing F1 seeds derived from reciprocally crossed BnFAE1-Ri lines and high erucic acid cultivars. In addition, F1 seeds derived from reciprocal crosses between BnFAE1-Ri lines and high erucic acid cultivars showed significantly increased oleic acid (more than 52%) and sharply decreased erucic acid (less than 4%), demonstrating that the RNAi construct of BnFAE1 can effectively interfere with the target gene in F1 seeds. Taken together, our results demonstrate that BnFAE1 is a reliable target for genetic improvement of rapeseed in seed oil quality promotion.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.

Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.

Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.

Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.

Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.

Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.

Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.

Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China. Electronic address: yznszdz@mail.zaas.ac.cn.

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26381181

Citation

Shi, Jianghua, et al. "RNAi Knockdown of Fatty Acid Elongase1 Alters Fatty Acid Composition in Brassica Napus." Biochemical and Biophysical Research Communications, vol. 466, no. 3, 2015, pp. 518-22.

Shi J, Lang C, Wu X, et al. RNAi knockdown of fatty acid elongase1 alters fatty acid composition in Brassica napus. Biochem Biophys Res Commun. 2015;466(3):518-22.

Shi, J., Lang, C., Wu, X., Liu, R., Zheng, T., Zhang, D., Chen, J., & Wu, G. (2015). RNAi knockdown of fatty acid elongase1 alters fatty acid composition in Brassica napus. Biochemical and Biophysical Research Communications, 466(3), 518-22. https://doi.org/10.1016/j.bbrc.2015.09.062

Shi J, et al. RNAi Knockdown of Fatty Acid Elongase1 Alters Fatty Acid Composition in Brassica Napus. Biochem Biophys Res Commun. 2015 Oct 23;466(3):518-22. PubMed PMID: 26381181.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - RNAi knockdown of fatty acid elongase1 alters fatty acid composition in Brassica napus. AU - Shi,Jianghua, AU - Lang,Chunxiu, AU - Wu,Xuelong, AU - Liu,Renhu, AU - Zheng,Tao, AU - Zhang,Dongqing, AU - Chen,Jinqing, AU - Wu,Guanting, Y1 - 2015/09/14/ PY - 2015/09/07/received PY - 2015/09/10/accepted PY - 2015/9/19/entrez PY - 2015/9/19/pubmed PY - 2016/4/19/medline KW - BnFAE1 KW - Erucic acid KW - Oleic acid KW - RNAi knockdown SP - 518 EP - 22 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 466 IS - 3 N2 - The quality and end-use of oil from oilseed crops is determined by its fatty acid composition. In particular, the relative proportions of erucic and oleic acids are key selection traits for breeders. The goal of our research is to genetically improve the nutritional quality of Brassica napus cultivar CY2, the oil of which is high in erucic acid (about 40%) and low in oleic acid (about 20%). Here, we report the use of a seed-specific napin A promoter to drive the knockdown of BnFAE1 in transgenic CY2. Southern blotting results confirmed the presence of the transgene. RT-PCR analysis showed that the levels of BnFAE1 were greatly decreased in BnFAE1-Ri lines compared with the CY2 cultivar. Knockdown of BnFAE1 sharply decreased the levels of erucic acid (less than 3%), largely increased the contents of oleic acid (more than 60%) and slightly increased the polyunsaturated chain fatty acids. Compared with high erucic acid parents, expression of BnFAE1 was dramatically decreased in developing F1 seeds derived from reciprocally crossed BnFAE1-Ri lines and high erucic acid cultivars. In addition, F1 seeds derived from reciprocal crosses between BnFAE1-Ri lines and high erucic acid cultivars showed significantly increased oleic acid (more than 52%) and sharply decreased erucic acid (less than 4%), demonstrating that the RNAi construct of BnFAE1 can effectively interfere with the target gene in F1 seeds. Taken together, our results demonstrate that BnFAE1 is a reliable target for genetic improvement of rapeseed in seed oil quality promotion. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/26381181/RNAi_knockdown_of_fatty_acid_elongase1_alters_fatty_acid_composition_in_Brassica_napus_ DB - PRIME DP - Unbound Medicine ER -