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Involvement of acid-sensing ion channel 1a in matrix metabolism of endplate chondrocytes under extracellular acidic conditions through NF-κB transcriptional activity.
Cell Stress Chaperones 2016; 21(1):97-104CS

Abstract

Acidic conditions are present in degenerated intervertebral discs and are believed to be responsible for matrix breakdown. Acid-sensing ion channel 1a (ASIC1a) is expressed in endplate chondrocytes, and its activation is associated with endplate chondrocyte apoptosis. However, the precise role of ASIC1a in regulating the matrix metabolic activity of endplate chondrocytes in response to extracellular acid remains poorly understood. Aggrecan (ACAN), type II collagen (Col2a1), and matrix metalloproteinase (MMP) expressions were determined using reverse transcription (RT)-PCR and Western blot. ASIC1a was knocked down by transfecting endplate chondrocytes with ASIC1a siRNA. MMP activity and NF-κB transcriptional activity were measured. NF-κB transcriptional activity was assessed by examining cytosolic phosphorylated IκBα and nuclear phosphorylated p65 levels. Extracellular acidic solution (pH 6.0) resulted in a decrease in ACAN and Co12a1 expressions and an increase in MMP-1, MMP-9, and MMP-13 expressions, as well as in MMP activity; while ASIC1a siRNA blocked these effects. In addition, acid-induced increase in cytosolic levels of phosphorylated IκBα and nuclear levels of phosphorylated p65 in endplate chondrocytes were inhibited by ASIC1a siRNA. ASIC1a is involved in matrix metabolism of endplate chondrocytes under extracellular acidic conditions via NF-κB transcriptional activity.

Authors+Show Affiliations

Department of Orthopaedics and Central Laboratory, The Third Hospital Affiliated to Nantong University, Wuxi, Jiangsu, 214041, China.Department of Orthopaedics, Jinshan Hospital, Fudan University, Shanghai, 201508, China. zhaomingdong@medmail.com.cn.Department of Orthopaedics and Central Laboratory, The Third Hospital Affiliated to Nantong University, Wuxi, Jiangsu, 214041, China.Department of Orthopaedics and Central Laboratory, The Third Hospital Affiliated to Nantong University, Wuxi, Jiangsu, 214041, China.Department of Internal Medicine, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong, 266100, China.Department of Orthopaedics and Central Laboratory, The Third Hospital Affiliated to Nantong University, Wuxi, Jiangsu, 214041, China.The Second Affiliated Hospital of Anhui Medical University, Hefei, 230601, Anhui, China.Department of Orthopaedics and Central Laboratory, The Third Hospital Affiliated to Nantong University, Wuxi, Jiangsu, 214041, China. lixia.ahmu.cn@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26384841

Citation

Yuan, Feng-Lai, et al. "Involvement of Acid-sensing Ion Channel 1a in Matrix Metabolism of Endplate Chondrocytes Under Extracellular Acidic Conditions Through NF-κB Transcriptional Activity." Cell Stress & Chaperones, vol. 21, no. 1, 2016, pp. 97-104.
Yuan FL, Zhao MD, Jiang DL, et al. Involvement of acid-sensing ion channel 1a in matrix metabolism of endplate chondrocytes under extracellular acidic conditions through NF-κB transcriptional activity. Cell Stress Chaperones. 2016;21(1):97-104.
Yuan, F. L., Zhao, M. D., Jiang, D. L., Jin, C., Liu, H. F., Xu, M. H., ... Li, X. (2016). Involvement of acid-sensing ion channel 1a in matrix metabolism of endplate chondrocytes under extracellular acidic conditions through NF-κB transcriptional activity. Cell Stress & Chaperones, 21(1), pp. 97-104. doi:10.1007/s12192-015-0643-7.
Yuan FL, et al. Involvement of Acid-sensing Ion Channel 1a in Matrix Metabolism of Endplate Chondrocytes Under Extracellular Acidic Conditions Through NF-κB Transcriptional Activity. Cell Stress Chaperones. 2016;21(1):97-104. PubMed PMID: 26384841.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of acid-sensing ion channel 1a in matrix metabolism of endplate chondrocytes under extracellular acidic conditions through NF-κB transcriptional activity. AU - Yuan,Feng-Lai, AU - Zhao,Ming-Dong, AU - Jiang,Dong-Lin, AU - Jin,Cheng, AU - Liu,Hai-Fei, AU - Xu,Ming-Hui, AU - Hu,Wei, AU - Li,Xia, Y1 - 2015/09/18/ PY - 2015/06/30/received PY - 2015/09/04/accepted PY - 2015/09/01/revised PY - 2015/9/20/entrez PY - 2015/9/20/pubmed PY - 2016/9/27/medline KW - Acid-sensing ion channel 1a KW - Endplate chondrocytes KW - Extracellular pH KW - Metalloproteinases SP - 97 EP - 104 JF - Cell stress & chaperones JO - Cell Stress Chaperones VL - 21 IS - 1 N2 - Acidic conditions are present in degenerated intervertebral discs and are believed to be responsible for matrix breakdown. Acid-sensing ion channel 1a (ASIC1a) is expressed in endplate chondrocytes, and its activation is associated with endplate chondrocyte apoptosis. However, the precise role of ASIC1a in regulating the matrix metabolic activity of endplate chondrocytes in response to extracellular acid remains poorly understood. Aggrecan (ACAN), type II collagen (Col2a1), and matrix metalloproteinase (MMP) expressions were determined using reverse transcription (RT)-PCR and Western blot. ASIC1a was knocked down by transfecting endplate chondrocytes with ASIC1a siRNA. MMP activity and NF-κB transcriptional activity were measured. NF-κB transcriptional activity was assessed by examining cytosolic phosphorylated IκBα and nuclear phosphorylated p65 levels. Extracellular acidic solution (pH 6.0) resulted in a decrease in ACAN and Co12a1 expressions and an increase in MMP-1, MMP-9, and MMP-13 expressions, as well as in MMP activity; while ASIC1a siRNA blocked these effects. In addition, acid-induced increase in cytosolic levels of phosphorylated IκBα and nuclear levels of phosphorylated p65 in endplate chondrocytes were inhibited by ASIC1a siRNA. ASIC1a is involved in matrix metabolism of endplate chondrocytes under extracellular acidic conditions via NF-κB transcriptional activity. SN - 1466-1268 UR - https://www.unboundmedicine.com/medline/citation/26384841/Involvement_of_acid_sensing_ion_channel_1a_in_matrix_metabolism_of_endplate_chondrocytes_under_extracellular_acidic_conditions_through_NF_κB_transcriptional_activity_ L2 - https://dx.doi.org/10.1007/s12192-015-0643-7 DB - PRIME DP - Unbound Medicine ER -