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Effects of antimicrobial peptides (AMPs) on blood biochemical parameters, antioxidase activity, and immune function in the common carp (Cyprinus carpio).
Fish Shellfish Immunol. 2015 Nov; 47(1):429-34.FS

Abstract

Antibiotic use in livestock feed additives has resulted in harmful residue accumulation and spread of drug-resistance. We examined the use of antimicrobial peptides (AMPs) as a safer alternative to antibiotics in feeding the common carp. AMPs were added to common carp basal diets (Control) as additives at four concentrations: 100 mg kg(-1) (B1), 200 mg kg(-1) (B2), 400 mg kg(-1) (B3), 600 mg kg(-1) (B4) by dry weight of basal diet. After a 60-day feeding experiment, the final weight, DG and SGR of carps on B1, B2 and B3 diet were significantly higher than the control (p < 0.05). The FCR of carps on B1, B2 and B3 diet were significantly lower than the control (p < 0.05). Carps on B2, B3, and B4 diets showed significantly lower (p < 0.05) levels of triglyceride than the control. B4-fed carps showed significantly lower (p < 0.05) levels of total protein, albumin, and total cholesterol than the control. However there was no remarkable difference (p > 0.05) in levels of uric ammonia, globulin, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase, lactic dehydrogenase and blood glucose in all groups. The serum superoxide dismutase and catalase activity of B1-fed carps was significantly higher (p < 0.05) than the control and B4-fed carps. The serum alkaline phosphate activity of carps on B1 diets was significantly higher (p < 0.05) than B4-fed carps. The serum acid phosphatase activity of B1-fed carps was significantly higher (p < 0.05) than the control and other antimicrobial peptide-fed groups. The serum lysozyme activity of carps on B1, B2, and B3 diets was significantly higher (p < 0.05) than the control- and B4-fed carps. Regarding immune factors in serum, the levels of immunoglobulin (Ig) and interleukin (IL)-1β in B1-fed carps were significantly higher (p < 0.05) than the control and other groups, while IL-1α levels in B1-fed carps was significantly higher (p < 0.05) than the control-, B2-, and B3-fed carps. Furthermore, there were no significant differences in the content of MHC among the five groups. In conclusion, antimicrobial peptide can reduce triglyceride levels in serum, enrich oxidation resistance, and improve immunity of the common carp. It showed that appropriate concentration of antibacterial peptide as supplements in diets for common carp increased the final weight, DG, SGR and decreased the FCR.

Authors+Show Affiliations

Faculty of Animal Science and Technology, Jilin Agricultural University, Changchun, China.Faculty of Animal Science and Technology, Jilin Agricultural University, Changchun, China. Electronic address: dongmignzhang0431@aliyun.com.Faculty of Animal Science and Technology, Jilin Agricultural University, Changchun, China.Faculty of Animal Science and Technology, Jilin Agricultural University, Changchun, China.Faculty of Animal Science and Technology, Jilin Agricultural University, Changchun, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26386195

Citation

Dong, Xiao-Qing, et al. "Effects of Antimicrobial Peptides (AMPs) On Blood Biochemical Parameters, Antioxidase Activity, and Immune Function in the Common Carp (Cyprinus Carpio)." Fish & Shellfish Immunology, vol. 47, no. 1, 2015, pp. 429-34.
Dong XQ, Zhang DM, Chen YK, et al. Effects of antimicrobial peptides (AMPs) on blood biochemical parameters, antioxidase activity, and immune function in the common carp (Cyprinus carpio). Fish Shellfish Immunol. 2015;47(1):429-34.
Dong, X. Q., Zhang, D. M., Chen, Y. K., Wang, Q. J., & Yang, Y. Y. (2015). Effects of antimicrobial peptides (AMPs) on blood biochemical parameters, antioxidase activity, and immune function in the common carp (Cyprinus carpio). Fish & Shellfish Immunology, 47(1), 429-34. https://doi.org/10.1016/j.fsi.2015.09.030
Dong XQ, et al. Effects of Antimicrobial Peptides (AMPs) On Blood Biochemical Parameters, Antioxidase Activity, and Immune Function in the Common Carp (Cyprinus Carpio). Fish Shellfish Immunol. 2015;47(1):429-34. PubMed PMID: 26386195.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of antimicrobial peptides (AMPs) on blood biochemical parameters, antioxidase activity, and immune function in the common carp (Cyprinus carpio). AU - Dong,Xiao-Qing, AU - Zhang,Dong-Ming, AU - Chen,Yu-Ke, AU - Wang,Qiu-Ju, AU - Yang,Yi-Yu, Y1 - 2015/09/18/ PY - 2015/07/20/received PY - 2015/09/10/revised PY - 2015/09/14/accepted PY - 2015/9/20/entrez PY - 2015/9/20/pubmed PY - 2016/8/11/medline KW - Antimicrobial peptides KW - Antioxidase activity KW - Blood biochemical parameter KW - Common carp (Cyprinus carpio) KW - Immunity SP - 429 EP - 34 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 47 IS - 1 N2 - Antibiotic use in livestock feed additives has resulted in harmful residue accumulation and spread of drug-resistance. We examined the use of antimicrobial peptides (AMPs) as a safer alternative to antibiotics in feeding the common carp. AMPs were added to common carp basal diets (Control) as additives at four concentrations: 100 mg kg(-1) (B1), 200 mg kg(-1) (B2), 400 mg kg(-1) (B3), 600 mg kg(-1) (B4) by dry weight of basal diet. After a 60-day feeding experiment, the final weight, DG and SGR of carps on B1, B2 and B3 diet were significantly higher than the control (p < 0.05). The FCR of carps on B1, B2 and B3 diet were significantly lower than the control (p < 0.05). Carps on B2, B3, and B4 diets showed significantly lower (p < 0.05) levels of triglyceride than the control. B4-fed carps showed significantly lower (p < 0.05) levels of total protein, albumin, and total cholesterol than the control. However there was no remarkable difference (p > 0.05) in levels of uric ammonia, globulin, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase, lactic dehydrogenase and blood glucose in all groups. The serum superoxide dismutase and catalase activity of B1-fed carps was significantly higher (p < 0.05) than the control and B4-fed carps. The serum alkaline phosphate activity of carps on B1 diets was significantly higher (p < 0.05) than B4-fed carps. The serum acid phosphatase activity of B1-fed carps was significantly higher (p < 0.05) than the control and other antimicrobial peptide-fed groups. The serum lysozyme activity of carps on B1, B2, and B3 diets was significantly higher (p < 0.05) than the control- and B4-fed carps. Regarding immune factors in serum, the levels of immunoglobulin (Ig) and interleukin (IL)-1β in B1-fed carps were significantly higher (p < 0.05) than the control and other groups, while IL-1α levels in B1-fed carps was significantly higher (p < 0.05) than the control-, B2-, and B3-fed carps. Furthermore, there were no significant differences in the content of MHC among the five groups. In conclusion, antimicrobial peptide can reduce triglyceride levels in serum, enrich oxidation resistance, and improve immunity of the common carp. It showed that appropriate concentration of antibacterial peptide as supplements in diets for common carp increased the final weight, DG, SGR and decreased the FCR. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/26386195/Effects_of_antimicrobial_peptides__AMPs__on_blood_biochemical_parameters_antioxidase_activity_and_immune_function_in_the_common_carp__Cyprinus_carpio__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(15)30155-8 DB - PRIME DP - Unbound Medicine ER -