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A novel paper-based device coupled with a silver nanoparticle-modified boron-doped diamond electrode for cholesterol detection.
Anal Chim Acta. 2015 Sep 03; 891:136-43.AC

Abstract

A novel paper-based analytical device (PAD) coupled with a silver nanoparticle-modified boron-doped diamond (AgNP/BDD) electrode was first developed as a cholesterol sensor. The AgNP/BDD electrode was used as working electrode after modification by AgNPs using an electrodeposition method. Wax printing was used to define the hydrophilic and hydrophobic areas on filter paper, and then counter and reference electrodes were fabricated on the hydrophilic area by screen-printing in house. For the amperometric detection, cholesterol and cholesterol oxidase (ChOx) were directly drop-cast onto the hydrophilic area, and H2O2 produced from the enzymatic reaction was monitored. The fabricated device demonstrated a good linearity (0.39 mg dL(-1) to 270.69 mg dL(-1)), low detection limit (0.25 mg dL(-1)), and high sensitivity (49.61 μA mM(-1) cm(-2)). The precision value for ten replicates was 3.76% RSD for 1 mM H2O2. In addition, this biosensor exhibited very high selectivity for cholesterol detection and excellent recoveries for bovine serum analysis (in the range of 99.6-100.8%). The results showed that this new sensing platform will be an alternative tool for cholesterol detection in routine diagnosis and offers the advantages of low sample/reagent consumption, low cost, portability, and short analysis time.

Authors+Show Affiliations

Electrochemistry and Optical Spectroscopy Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok 10330, Thailand.Electrochemistry and Optical Spectroscopy Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok 10330, Thailand; Center for Petroleum, Petrochemicals and Advanced Materials, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok 10330, Thailand. Electronic address: corawon@chula.ac.th.Department of Chemistry, Faculty of Science, Srinakharinwirot University, Sukhumvit 23, Wattanna, Bangkok 10110, Thailand. Electronic address: weenasi@hotmail.com.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26388372

Citation

Nantaphol, Siriwan, et al. "A Novel Paper-based Device Coupled With a Silver Nanoparticle-modified Boron-doped Diamond Electrode for Cholesterol Detection." Analytica Chimica Acta, vol. 891, 2015, pp. 136-43.
Nantaphol S, Chailapakul O, Siangproh W. A novel paper-based device coupled with a silver nanoparticle-modified boron-doped diamond electrode for cholesterol detection. Anal Chim Acta. 2015;891:136-43.
Nantaphol, S., Chailapakul, O., & Siangproh, W. (2015). A novel paper-based device coupled with a silver nanoparticle-modified boron-doped diamond electrode for cholesterol detection. Analytica Chimica Acta, 891, 136-43. https://doi.org/10.1016/j.aca.2015.08.007
Nantaphol S, Chailapakul O, Siangproh W. A Novel Paper-based Device Coupled With a Silver Nanoparticle-modified Boron-doped Diamond Electrode for Cholesterol Detection. Anal Chim Acta. 2015 Sep 3;891:136-43. PubMed PMID: 26388372.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel paper-based device coupled with a silver nanoparticle-modified boron-doped diamond electrode for cholesterol detection. AU - Nantaphol,Siriwan, AU - Chailapakul,Orawon, AU - Siangproh,Weena, Y1 - 2015/08/22/ PY - 2015/05/06/received PY - 2015/07/08/revised PY - 2015/08/10/accepted PY - 2015/9/22/entrez PY - 2015/9/22/pubmed PY - 2016/6/25/medline KW - Boron-doped diamond electrode KW - Cholesterol KW - Electrochemical detection KW - Paper-based device KW - Silver nanoparticle SP - 136 EP - 43 JF - Analytica chimica acta JO - Anal Chim Acta VL - 891 N2 - A novel paper-based analytical device (PAD) coupled with a silver nanoparticle-modified boron-doped diamond (AgNP/BDD) electrode was first developed as a cholesterol sensor. The AgNP/BDD electrode was used as working electrode after modification by AgNPs using an electrodeposition method. Wax printing was used to define the hydrophilic and hydrophobic areas on filter paper, and then counter and reference electrodes were fabricated on the hydrophilic area by screen-printing in house. For the amperometric detection, cholesterol and cholesterol oxidase (ChOx) were directly drop-cast onto the hydrophilic area, and H2O2 produced from the enzymatic reaction was monitored. The fabricated device demonstrated a good linearity (0.39 mg dL(-1) to 270.69 mg dL(-1)), low detection limit (0.25 mg dL(-1)), and high sensitivity (49.61 μA mM(-1) cm(-2)). The precision value for ten replicates was 3.76% RSD for 1 mM H2O2. In addition, this biosensor exhibited very high selectivity for cholesterol detection and excellent recoveries for bovine serum analysis (in the range of 99.6-100.8%). The results showed that this new sensing platform will be an alternative tool for cholesterol detection in routine diagnosis and offers the advantages of low sample/reagent consumption, low cost, portability, and short analysis time. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/26388372/A_novel_paper_based_device_coupled_with_a_silver_nanoparticle_modified_boron_doped_diamond_electrode_for_cholesterol_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(15)00985-X DB - PRIME DP - Unbound Medicine ER -