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Synergic hypocholesterolaemic effect of n-3 PUFA and oestrogen by modulation of hepatic cholesterol metabolism in female rats.
Br J Nutr. 2015 Dec 14; 114(11):1766-73.BJ

Abstract

n-3 PUFA such as EPA and DHA as well as oestrogen have been reported to decrease blood levels of cholesterol, but their underlying mechanism is unclear. The purpose of this study was to determine the effects of the combination of n-3 PUFA supplementation and oestrogen injection on hepatic cholesterol metabolism. Rats were fed a modified AIN-93G diet with 0, 1 or 2 % n-3 PUFA (EPA+DHA) relative to the total energy intake for 12 weeks. Rats were surgically ovariectomised at week 8, and, after 1-week recovery, rats were injected with 17β-oestradiol-3-benzoate (E2) or maize oil for the last 3 weeks. Supplementation with n-3 PUFA and E2 injection significantly increased the ratio of the hepatic expression of phosphorylated AMP activated protein kinase (p-AMPK):AMP activated protein kinase (AMPK) and decreased sterol regulatory element-binding protein-2, 3-hydroxy-3-methylglutaryl coenzyme A reductase and proprotein convertase subtilisin/kexin type 9. Supplementation with n-3 PUFA increased hepatic expression of cholesterol 7α-hydroxylase (CYP7A1), sterol 12α-hydroxylase (CYP8B1) and sterol 27-hydroxylase (CYP27A1); however, E2 injection decreased CYP7A1 and CYP8B1 but not CYP27A1. Additionally, E2 injection increased hepatic expression of oestrogen receptor-α and β. In conclusion, n-3 PUFA supplementation and E2 injection had synergic hypocholesterolaemic effects by down-regulating hepatic cholesterol synthesis (n-3 PUFA and oestrogen) and up-regulating bile acid synthesis (n-3 PUFA) in ovariectomised rats.

Authors+Show Affiliations

Department of Food and Nutrition,Hanyang University,Seoul 133-791,South Korea.Department of Food and Nutrition,Hanyang University,Seoul 133-791,South Korea.Department of Food and Nutrition,Hanyang University,Seoul 133-791,South Korea.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26388416

Citation

Oh, Yuna, et al. "Synergic Hypocholesterolaemic Effect of N-3 PUFA and Oestrogen By Modulation of Hepatic Cholesterol Metabolism in Female Rats." The British Journal of Nutrition, vol. 114, no. 11, 2015, pp. 1766-73.
Oh Y, Jin Y, Park Y. Synergic hypocholesterolaemic effect of n-3 PUFA and oestrogen by modulation of hepatic cholesterol metabolism in female rats. Br J Nutr. 2015;114(11):1766-73.
Oh, Y., Jin, Y., & Park, Y. (2015). Synergic hypocholesterolaemic effect of n-3 PUFA and oestrogen by modulation of hepatic cholesterol metabolism in female rats. The British Journal of Nutrition, 114(11), 1766-73. https://doi.org/10.1017/S0007114515003517
Oh Y, Jin Y, Park Y. Synergic Hypocholesterolaemic Effect of N-3 PUFA and Oestrogen By Modulation of Hepatic Cholesterol Metabolism in Female Rats. Br J Nutr. 2015 Dec 14;114(11):1766-73. PubMed PMID: 26388416.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synergic hypocholesterolaemic effect of n-3 PUFA and oestrogen by modulation of hepatic cholesterol metabolism in female rats. AU - Oh,Yuna, AU - Jin,Youri, AU - Park,Yongsoon, Y1 - 2015/09/21/ PY - 2015/9/22/entrez PY - 2015/9/22/pubmed PY - 2016/2/19/medline KW - n-3 PUFA KW - AMPK AMP activated protein kinase KW - CYP27A1 sterol 27-hydroxylase KW - CYP7A1 cholesterol 7α-hydroxylase KW - CYP8B1 sterol 12α-hydroxylase KW - Cholesterol metabolism KW - E2 17β-oestradiol-3-benzoate KW - ER-α oestrogen receptor-α KW - ER-β oestrogen receptor-β KW - HMG-CoA reductase 3-hydroxy-3-methylglutaryl coenzyme A reductase KW - Oestrogen KW - Ovariectomised rats KW - Proprotein convertase subtilisin/kexin type 9 KW - SREBP-2 sterol regulatory element-binding protein-2 KW - TC total cholesterol KW - p-AMPK phosphorylated AMP activated protein kinase SP - 1766 EP - 73 JF - The British journal of nutrition JO - Br. J. Nutr. VL - 114 IS - 11 N2 - n-3 PUFA such as EPA and DHA as well as oestrogen have been reported to decrease blood levels of cholesterol, but their underlying mechanism is unclear. The purpose of this study was to determine the effects of the combination of n-3 PUFA supplementation and oestrogen injection on hepatic cholesterol metabolism. Rats were fed a modified AIN-93G diet with 0, 1 or 2 % n-3 PUFA (EPA+DHA) relative to the total energy intake for 12 weeks. Rats were surgically ovariectomised at week 8, and, after 1-week recovery, rats were injected with 17β-oestradiol-3-benzoate (E2) or maize oil for the last 3 weeks. Supplementation with n-3 PUFA and E2 injection significantly increased the ratio of the hepatic expression of phosphorylated AMP activated protein kinase (p-AMPK):AMP activated protein kinase (AMPK) and decreased sterol regulatory element-binding protein-2, 3-hydroxy-3-methylglutaryl coenzyme A reductase and proprotein convertase subtilisin/kexin type 9. Supplementation with n-3 PUFA increased hepatic expression of cholesterol 7α-hydroxylase (CYP7A1), sterol 12α-hydroxylase (CYP8B1) and sterol 27-hydroxylase (CYP27A1); however, E2 injection decreased CYP7A1 and CYP8B1 but not CYP27A1. Additionally, E2 injection increased hepatic expression of oestrogen receptor-α and β. In conclusion, n-3 PUFA supplementation and E2 injection had synergic hypocholesterolaemic effects by down-regulating hepatic cholesterol synthesis (n-3 PUFA and oestrogen) and up-regulating bile acid synthesis (n-3 PUFA) in ovariectomised rats. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/26388416/Synergic_hypocholesterolaemic_effect_of_n_3_PUFA_and_oestrogen_by_modulation_of_hepatic_cholesterol_metabolism_in_female_rats_ L2 - https://www.cambridge.org/core/product/identifier/S0007114515003517/type/journal_article DB - PRIME DP - Unbound Medicine ER -