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Survey of Omp19 immunogenicity against Brucella abortus and Brucella melitensis: influence of nanoparticulation versus traditional immunization.
Vet Res Commun 2015; 39(4):217-28VR

Abstract

Brucellosis is the most common zoonotic bacterial disease. Prevention of human brucellosis is achieved through pasteurization of dairy products, appropriate sanitation and vaccination of domestic animals against the Brucella species. B. abortus unlipidated 19 kDa outer membrane protein (U-Omp19) is a promising candidate for a subunit vaccine against brucellosis. This study investigates immunogenicity of Omp19 alone and with Freund's adjuvant (Omp19-IFA) and N-trimethyl chitosan (TMC/Omp19) nanoparticles, as well as the effect of Omp19 administration route on immunological responses and protection. The omp19 gene was expressed in E. coli BL21 (DE3). After purification, the recombinant Omp19 was loaded onto TMC nanoparticles by ionic gelation with tripolyphosphate. Particle size and loading efficiency of the nanoparticles were determined. Omp19-IFA was administered intraperitoneally while TMC/Omp19 nanoparticles were administered orally and intraperitoneally. The results indicated that intraperitoneal (i.p.) immunization by Omp19-IFA and TMC/Omp19 nanoparticles induced Th1 and Th2 immune responses, respectively, whereas oral immunization of TMC/Omp19 nanoparticles induced a mixed Th1/Th17 immune response. Moreover, oral immunization increased IgA levels in feces. Immunized mice were challenged with virulent B. melitensis 16 M and B. abortus 544. Oral immunization with TMC/Omp19 nanoparticles induced a remarkably high protection level against B. melitensis and B. abortus. The results showed that immunization route has a pivotal role in immune response polarization and protective efficiency of Omp19 antigen. Also, it was deduced that the higher protection level achieved through oral administration of TMC/Omp19 nanoparticles may be due to the elicited Th17 response.

Authors+Show Affiliations

Department of Molecular Genetics, Faculty of Basic Sciences, Tarbiat Modarres University, Tehran, Iran.Department of Clinical Biochemistry, Faculty of Medicine, Tarbiat Modares University, P.O. Box: 14155-6343, Tehran, Iran. lotfi_ab@modares.ac.ir.Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Vanak Sq. Molasadra St., P.O. Box 19395-5487, Tehran, Iran. Jafar.amani@gmail.com.Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Vanak Sq. Molasadra St., P.O. Box 19395-5487, Tehran, Iran. Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.Department of Microbiology & Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.Brucellosis Department, Razi Vaccine and Serum Research Institute, Karaj, Iran.Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26395469

Citation

Abkar, Morteza, et al. "Survey of Omp19 Immunogenicity Against Brucella Abortus and Brucella Melitensis: Influence of Nanoparticulation Versus Traditional Immunization." Veterinary Research Communications, vol. 39, no. 4, 2015, pp. 217-28.
Abkar M, Lotfi AS, Amani J, et al. Survey of Omp19 immunogenicity against Brucella abortus and Brucella melitensis: influence of nanoparticulation versus traditional immunization. Vet Res Commun. 2015;39(4):217-28.
Abkar, M., Lotfi, A. S., Amani, J., Eskandari, K., Ramandi, M. F., Salimian, J., ... Koushki, H. (2015). Survey of Omp19 immunogenicity against Brucella abortus and Brucella melitensis: influence of nanoparticulation versus traditional immunization. Veterinary Research Communications, 39(4), pp. 217-28. doi:10.1007/s11259-015-9645-2.
Abkar M, et al. Survey of Omp19 Immunogenicity Against Brucella Abortus and Brucella Melitensis: Influence of Nanoparticulation Versus Traditional Immunization. Vet Res Commun. 2015;39(4):217-28. PubMed PMID: 26395469.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Survey of Omp19 immunogenicity against Brucella abortus and Brucella melitensis: influence of nanoparticulation versus traditional immunization. AU - Abkar,Morteza, AU - Lotfi,Abbas Sahebghadam, AU - Amani,Jafar, AU - Eskandari,Khadijeh, AU - Ramandi,Mehdi Fasihi, AU - Salimian,Jafar, AU - Brujeni,Gholamreza Nikbakht, AU - Alamian,Saeed, AU - Kamali,Mehdi, AU - Koushki,Hamid, Y1 - 2015/09/22/ PY - 2015/02/26/received PY - 2015/09/08/accepted PY - 2015/9/24/entrez PY - 2015/9/24/pubmed PY - 2016/8/11/medline KW - Brucellosis KW - Nanoparticles KW - Omp19 KW - Oral vaccine KW - Trimethyl chitosan SP - 217 EP - 28 JF - Veterinary research communications JO - Vet. Res. Commun. VL - 39 IS - 4 N2 - Brucellosis is the most common zoonotic bacterial disease. Prevention of human brucellosis is achieved through pasteurization of dairy products, appropriate sanitation and vaccination of domestic animals against the Brucella species. B. abortus unlipidated 19 kDa outer membrane protein (U-Omp19) is a promising candidate for a subunit vaccine against brucellosis. This study investigates immunogenicity of Omp19 alone and with Freund's adjuvant (Omp19-IFA) and N-trimethyl chitosan (TMC/Omp19) nanoparticles, as well as the effect of Omp19 administration route on immunological responses and protection. The omp19 gene was expressed in E. coli BL21 (DE3). After purification, the recombinant Omp19 was loaded onto TMC nanoparticles by ionic gelation with tripolyphosphate. Particle size and loading efficiency of the nanoparticles were determined. Omp19-IFA was administered intraperitoneally while TMC/Omp19 nanoparticles were administered orally and intraperitoneally. The results indicated that intraperitoneal (i.p.) immunization by Omp19-IFA and TMC/Omp19 nanoparticles induced Th1 and Th2 immune responses, respectively, whereas oral immunization of TMC/Omp19 nanoparticles induced a mixed Th1/Th17 immune response. Moreover, oral immunization increased IgA levels in feces. Immunized mice were challenged with virulent B. melitensis 16 M and B. abortus 544. Oral immunization with TMC/Omp19 nanoparticles induced a remarkably high protection level against B. melitensis and B. abortus. The results showed that immunization route has a pivotal role in immune response polarization and protective efficiency of Omp19 antigen. Also, it was deduced that the higher protection level achieved through oral administration of TMC/Omp19 nanoparticles may be due to the elicited Th17 response. SN - 1573-7446 UR - https://www.unboundmedicine.com/medline/citation/26395469/Survey_of_Omp19_immunogenicity_against_Brucella_abortus_and_Brucella_melitensis:_influence_of_nanoparticulation_versus_traditional_immunization_ L2 - https://doi.org/10.1007/s11259-015-9645-2 DB - PRIME DP - Unbound Medicine ER -