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Constitutive overexpression of cytochrome P450 associated with imidacloprid resistance in Laodelphax striatellus (Fallén).
Pest Manag Sci. 2016 May; 72(5):1051-8.PM

Abstract

BACKGROUND

Imidacloprid is a principal insecticide for controlling rice planthoppers worldwide. Resistance to imidacloprid has been reported in a field population of Laodelphax striatellus. The present work was conducted to study the molecular mechanisms of imidacloprid resistance.

RESULTS

An imidacloprid-resistant strain was produced by selecting a field population with imidacloprid for 24 generations. Piperonyl butoxide (PBO) showed a 1.70-fold synergistic effect. Enzyme activity assays were conducted, and cytochrome P450 monooxygenase showed 1.88-fold activity. The mRNA expression levels of 57 P450 genes were compared. Four CYP genes were found to be overexpressed and significantly different to the susceptible strain. Four strains were selected with imidacloprid for a short period, and the expression levels of ten identified detoxification genes were then compared. Only CYP353D1v2 overexpressed and was significantly different to the susceptible strain. Strong correlation was found between CYP353D1v2 expression levels and imidacloprid treatments. Additionally, gene-silencing RNAi via dsRNA feeding showed that depressing the expression of CYP353D1v2 could significantly enhance the sensitivity of L. striatellus to imidacloprid.

CONCLUSION

Constitutive overexpression of four CYP genes was associated with imidacloprid resistance in long-term selection, and expression of CYP353D1v2 with imidacloprid resistance in short-term selection in L. striatellus.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Elzaki ME
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Jiangsu/The Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing, 210095 Jiangsu, China.
Zhang W
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Jiangsu/The Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing, 210095 Jiangsu, China.
Feng A
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Jiangsu/The Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing, 210095 Jiangsu, China.
Qiou X
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Jiangsu/The Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing, 210095 Jiangsu, China.
Zhao W
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Jiangsu/The Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing, 210095 Jiangsu, China.
Han Z
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Jiangsu/The Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing, 210095 Jiangsu, China.

MeSH

AnimalsChinaCytochrome P-450 Enzyme SystemHemipteraImidazolesInsect ProteinsInsecticide ResistanceInsecticidesNeonicotinoidsNitro Compounds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26395964

Citation

Elzaki, Mohammed Esmail Abdalla, et al. "Constitutive Overexpression of Cytochrome P450 Associated With Imidacloprid Resistance in Laodelphax Striatellus (Fallén)." Pest Management Science, vol. 72, no. 5, 2016, pp. 1051-8.
Elzaki ME, Zhang W, Feng A, et al. Constitutive overexpression of cytochrome P450 associated with imidacloprid resistance in Laodelphax striatellus (Fallén). Pest Manag Sci. 2016;72(5):1051-8.
Elzaki, M. E., Zhang, W., Feng, A., Qiou, X., Zhao, W., & Han, Z. (2016). Constitutive overexpression of cytochrome P450 associated with imidacloprid resistance in Laodelphax striatellus (Fallén). Pest Management Science, 72(5), 1051-8. https://doi.org/10.1002/ps.4155
Elzaki ME, et al. Constitutive Overexpression of Cytochrome P450 Associated With Imidacloprid Resistance in Laodelphax Striatellus (Fallén). Pest Manag Sci. 2016;72(5):1051-8. PubMed PMID: 26395964.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Constitutive overexpression of cytochrome P450 associated with imidacloprid resistance in Laodelphax striatellus (Fallén). AU - Elzaki,Mohammed Esmail Abdalla, AU - Zhang,Wanfang, AU - Feng,Ai, AU - Qiou,Xiaoyan, AU - Zhao,Wanxue, AU - Han,Zhaojun, Y1 - 2015/11/04/ PY - 2014/08/22/received PY - 2015/09/12/revised PY - 2015/09/16/accepted PY - 2015/9/24/entrez PY - 2015/9/24/pubmed PY - 2016/12/16/medline KW - Laodelphax striatellus KW - RNA interference KW - constitutive overexpression KW - cytochrome P450 KW - imidacloprid SP - 1051 EP - 8 JF - Pest management science JO - Pest Manag Sci VL - 72 IS - 5 N2 - BACKGROUND: Imidacloprid is a principal insecticide for controlling rice planthoppers worldwide. Resistance to imidacloprid has been reported in a field population of Laodelphax striatellus. The present work was conducted to study the molecular mechanisms of imidacloprid resistance. RESULTS: An imidacloprid-resistant strain was produced by selecting a field population with imidacloprid for 24 generations. Piperonyl butoxide (PBO) showed a 1.70-fold synergistic effect. Enzyme activity assays were conducted, and cytochrome P450 monooxygenase showed 1.88-fold activity. The mRNA expression levels of 57 P450 genes were compared. Four CYP genes were found to be overexpressed and significantly different to the susceptible strain. Four strains were selected with imidacloprid for a short period, and the expression levels of ten identified detoxification genes were then compared. Only CYP353D1v2 overexpressed and was significantly different to the susceptible strain. Strong correlation was found between CYP353D1v2 expression levels and imidacloprid treatments. Additionally, gene-silencing RNAi via dsRNA feeding showed that depressing the expression of CYP353D1v2 could significantly enhance the sensitivity of L. striatellus to imidacloprid. CONCLUSION: Constitutive overexpression of four CYP genes was associated with imidacloprid resistance in long-term selection, and expression of CYP353D1v2 with imidacloprid resistance in short-term selection in L. striatellus. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/26395964/Constitutive_overexpression_of_cytochrome_P450_associated_with_imidacloprid_resistance_in_Laodelphax_striatellus__Fall��n__ DB - PRIME DP - Unbound Medicine ER -