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Effects of Microcystin-LR Exposure on Spermiogenesis in Nematode Caenorhabditis elegans.
Int J Mol Sci. 2015 Sep 22; 16(9):22927-37.IJ

Abstract

Little is known about the effect on spermiogenesis induced by microcystin-leucine arginine (MC-LR), even though such data are very important to better elucidate reproductive health. In the current work, with the aid of nematode Caenorhabditis elegans (C. elegans) as an animal model, we investigated the defects on spermiogenesis induced by MC-LR. Our results showed that MC-LR exposure induced sperm morphology abnormality and caused severe defects of sperm activation, trans-activation, sperm behavior and competition. Additionally, the expression levels of spe-15 were significantly decreased in C. elegans exposed to MC-LR lower than 16.0 μg/L, while the expression levels of spe-10 and fer-1 could be significantly lowered in C. elegans even exposed to 1.0 μg/L of MC-LR. Therefore, the present study reveals that MC-LR can induce adverse effects on spermiogenesis, and those defects of sperm functions may be induced by the decreases of spe-10, spe-15 and fer-1 gene expressions in C. elegans.

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Authors+Show Affiliations

Li Y
Key Laboratory of Environmental Medicine Engineering Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. yhli@seu.edu.cn.
Zhang M
Key Laboratory of Environmental Medicine Engineering Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. zhangminhui1990@163.com.
Chen P
Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA. pan.chen@einstein.yu.edu.
Liu R
Key Laboratory of Environmental Medicine Engineering Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. ranliu@seu.edu.cn.
Liang G
Key Laboratory of Environmental Medicine Engineering Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. lianggeyu@163.com.
Yin L
Key Laboratory of Environmental Medicine Engineering Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. lhyin@seu.edu.cn.
Pu Y
Key Laboratory of Environmental Medicine Engineering Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. yppu@seu.edu.cn.

MeSH

AnimalsCaenorhabditis elegansGene Expression Regulation, DevelopmentalMaleMarine ToxinsMicrocystinsSperm MotilitySpermatogenesisSpermatozoa

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26402677

Citation

Li, Yunhui, et al. "Effects of Microcystin-LR Exposure On Spermiogenesis in Nematode Caenorhabditis Elegans." International Journal of Molecular Sciences, vol. 16, no. 9, 2015, pp. 22927-37.
Li Y, Zhang M, Chen P, et al. Effects of Microcystin-LR Exposure on Spermiogenesis in Nematode Caenorhabditis elegans. Int J Mol Sci. 2015;16(9):22927-37.
Li, Y., Zhang, M., Chen, P., Liu, R., Liang, G., Yin, L., & Pu, Y. (2015). Effects of Microcystin-LR Exposure on Spermiogenesis in Nematode Caenorhabditis elegans. International Journal of Molecular Sciences, 16(9), 22927-37. https://doi.org/10.3390/ijms160922927
Li Y, et al. Effects of Microcystin-LR Exposure On Spermiogenesis in Nematode Caenorhabditis Elegans. Int J Mol Sci. 2015 Sep 22;16(9):22927-37. PubMed PMID: 26402677.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of Microcystin-LR Exposure on Spermiogenesis in Nematode Caenorhabditis elegans. AU - Li,Yunhui, AU - Zhang,Minhui, AU - Chen,Pan, AU - Liu,Ran, AU - Liang,Geyu, AU - Yin,Lihong, AU - Pu,Yuepu, Y1 - 2015/09/22/ PY - 2015/06/13/received PY - 2015/08/17/revised PY - 2015/09/15/accepted PY - 2015/9/25/entrez PY - 2015/9/25/pubmed PY - 2016/7/22/medline KW - Caenorhabditis elegans KW - microcystin-LR KW - reproductive toxicity KW - spermiogenesis SP - 22927 EP - 37 JF - International journal of molecular sciences JO - Int J Mol Sci VL - 16 IS - 9 N2 - Little is known about the effect on spermiogenesis induced by microcystin-leucine arginine (MC-LR), even though such data are very important to better elucidate reproductive health. In the current work, with the aid of nematode Caenorhabditis elegans (C. elegans) as an animal model, we investigated the defects on spermiogenesis induced by MC-LR. Our results showed that MC-LR exposure induced sperm morphology abnormality and caused severe defects of sperm activation, trans-activation, sperm behavior and competition. Additionally, the expression levels of spe-15 were significantly decreased in C. elegans exposed to MC-LR lower than 16.0 μg/L, while the expression levels of spe-10 and fer-1 could be significantly lowered in C. elegans even exposed to 1.0 μg/L of MC-LR. Therefore, the present study reveals that MC-LR can induce adverse effects on spermiogenesis, and those defects of sperm functions may be induced by the decreases of spe-10, spe-15 and fer-1 gene expressions in C. elegans. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/26402677/Effects_of_Microcystin_LR_Exposure_on_Spermiogenesis_in_Nematode_Caenorhabditis_elegans_ DB - PRIME DP - Unbound Medicine ER -