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In vivo photoprotective effects of cosmetic formulations containing UV filters, vitamins, Ginkgo biloba and red algae extracts.
J Photochem Photobiol B. 2015 Dec; 153:121-6.JP

Abstract

The aim of this study was to assess the photoprotective effects of cosmetic formulations containing UV filters, red algae, Porphyra umbilicalis, extracts and combinations of the extract with vitamins and Ginkgo biloba through the use of in vivo preclinical studies. For this study, 4 groups of 4 hairless mice each were treated with topical formulations applied on the dorsum for 5 days as follows: group 1 - control (no treatment); group 2 - application of the formulation F (sunscreen formulation containing only UV filters); group 3 - application of the formulation FA (sunscreen formulation with red algae extract); and group 4 - application of the formulation FVGA (sunscreen formulation with red algae extract, G. biloba and vitamins A, C and E). The effects of these formulations were evaluated by determining the transepidermal water loss (TEWL) and erythema index. Apoptosis was detected by immunohistochemical staining with anti-p53 and anti-caspase-3 antibodies. The results showed that the formulations protected the skin from erythema when exposed to UV radiation. The group that received the formulation FVGA presented a greater TEWL than did the other groups, suggesting that this formulation was involved in cell renewal. Immunohistochemical analysis showed that UV radiation caused an increase in the expression of p53 and active caspase-3, confirming that the damage caused by UV radiation exposure led to apoptosis. The application of all formulations studied resulted in a statistically significant reduction in the expression of p53 and caspase-3, with a more pronounced effect observed following treatment with FA. In conclusion, extracts from the red algae P. umbilicalis could be considered effective ingredients to be used in sunscreen formulations. The combination of vitamins A, E, C and G. biloba along with red algae extracts can improve significantly the performance of the sunscreens, preventing UV-induced DNA damage and inflammation. Thus, they should be considered an interesting combination for an effective photoprotective formulation with anti-aging properties.

Authors+Show Affiliations

Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. do Café S/N, Monte Alegre, 14040-903 Ribeirão Preto, SP, Brazil.Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. do Café S/N, Monte Alegre, 14040-903 Ribeirão Preto, SP, Brazil.Faculty of Medicine of Ribeirão Preto, University of São Paulo, Av. Bandeirantes, Brazil.Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. do Café S/N, Monte Alegre, 14040-903 Ribeirão Preto, SP, Brazil.Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. do Café S/N, Monte Alegre, 14040-903 Ribeirão Preto, SP, Brazil.Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. do Café S/N, Monte Alegre, 14040-903 Ribeirão Preto, SP, Brazil. Electronic address: pmcampos@usp.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26402714

Citation

Mercurio, D G., et al. "In Vivo Photoprotective Effects of Cosmetic Formulations Containing UV Filters, Vitamins, Ginkgo Biloba and Red Algae Extracts." Journal of Photochemistry and Photobiology. B, Biology, vol. 153, 2015, pp. 121-6.
Mercurio DG, Wagemaker TA, Alves VM, et al. In vivo photoprotective effects of cosmetic formulations containing UV filters, vitamins, Ginkgo biloba and red algae extracts. J Photochem Photobiol B. 2015;153:121-6.
Mercurio, D. G., Wagemaker, T. A., Alves, V. M., Benevenuto, C. G., Gaspar, L. R., & Maia Campos, P. M. (2015). In vivo photoprotective effects of cosmetic formulations containing UV filters, vitamins, Ginkgo biloba and red algae extracts. Journal of Photochemistry and Photobiology. B, Biology, 153, 121-6. https://doi.org/10.1016/j.jphotobiol.2015.09.016
Mercurio DG, et al. In Vivo Photoprotective Effects of Cosmetic Formulations Containing UV Filters, Vitamins, Ginkgo Biloba and Red Algae Extracts. J Photochem Photobiol B. 2015;153:121-6. PubMed PMID: 26402714.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo photoprotective effects of cosmetic formulations containing UV filters, vitamins, Ginkgo biloba and red algae extracts. AU - Mercurio,D G, AU - Wagemaker,T A L, AU - Alves,V M, AU - Benevenuto,C G, AU - Gaspar,L R, AU - Maia Campos,P M B G, Y1 - 2015/09/16/ PY - 2015/02/24/received PY - 2015/07/04/revised PY - 2015/09/14/accepted PY - 2015/9/25/entrez PY - 2015/9/25/pubmed PY - 2016/9/30/medline KW - Antioxidant substances KW - Cosmetics KW - Immunohistochemical analysis KW - Photoprotective KW - Preclinical studies SP - 121 EP - 6 JF - Journal of photochemistry and photobiology. B, Biology JO - J Photochem Photobiol B VL - 153 N2 - The aim of this study was to assess the photoprotective effects of cosmetic formulations containing UV filters, red algae, Porphyra umbilicalis, extracts and combinations of the extract with vitamins and Ginkgo biloba through the use of in vivo preclinical studies. For this study, 4 groups of 4 hairless mice each were treated with topical formulations applied on the dorsum for 5 days as follows: group 1 - control (no treatment); group 2 - application of the formulation F (sunscreen formulation containing only UV filters); group 3 - application of the formulation FA (sunscreen formulation with red algae extract); and group 4 - application of the formulation FVGA (sunscreen formulation with red algae extract, G. biloba and vitamins A, C and E). The effects of these formulations were evaluated by determining the transepidermal water loss (TEWL) and erythema index. Apoptosis was detected by immunohistochemical staining with anti-p53 and anti-caspase-3 antibodies. The results showed that the formulations protected the skin from erythema when exposed to UV radiation. The group that received the formulation FVGA presented a greater TEWL than did the other groups, suggesting that this formulation was involved in cell renewal. Immunohistochemical analysis showed that UV radiation caused an increase in the expression of p53 and active caspase-3, confirming that the damage caused by UV radiation exposure led to apoptosis. The application of all formulations studied resulted in a statistically significant reduction in the expression of p53 and caspase-3, with a more pronounced effect observed following treatment with FA. In conclusion, extracts from the red algae P. umbilicalis could be considered effective ingredients to be used in sunscreen formulations. The combination of vitamins A, E, C and G. biloba along with red algae extracts can improve significantly the performance of the sunscreens, preventing UV-induced DNA damage and inflammation. Thus, they should be considered an interesting combination for an effective photoprotective formulation with anti-aging properties. SN - 1873-2682 UR - https://www.unboundmedicine.com/medline/citation/26402714/In_vivo_photoprotective_effects_of_cosmetic_formulations_containing_UV_filters_vitamins_Ginkgo_biloba_and_red_algae_extracts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1011-1344(15)00307-3 DB - PRIME DP - Unbound Medicine ER -