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Identification of Low Molecular Weight Glutenin Alleles by Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF-MS) in Common Wheat (Triticum aestivum L.).
PLoS One. 2015; 10(9):e0138981.Plos

Abstract

Low molecular weight glutenin subunits (LMW-GS) play an important role in determining dough properties and breadmaking quality. However, resolution of the currently used methodologies for analyzing LMW-GS is rather low which prevents an efficient use of genetic variations associated with these alleles in wheat breeding. The aim of the current study is to evaluate and develop a rapid, simple, and accurate method to differentiate LMW-GS alleles using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A set of standard single LMW-GS allele lines as well as a suite of well documented wheat cultivars were collected from France, CIMMYT, and Canada. Method development and optimization were focused on protein extraction procedures and MALDI-TOF instrument settings to generate reproducible diagnostic spectrum peak profiles for each of the known wheat LMW-GS allele. Results revealed a total of 48 unique allele combinations among the studied genotypes. Characteristic MALDI-TOF peak patterns were obtained for 17 common LMW-GS alleles, including 5 (b, a or c, d, e, f), 7 (a, b, c, d or i, f, g, h) and 5 (a, b, c, d, f) patterns or alleles for the Glu-A3, Glu-B3, and Glu-D3 loci, respectively. In addition, some reproducible MALDI-TOF peak patterns were also obtained that did not match with any known alleles. The results demonstrated a high resolution and throughput nature of MALDI-TOF technology in analyzing LMW-GS alleles, which is suitable for application in wheat breeding programs in processing a large number of wheat lines with high accuracy in limited time. It also suggested that the variation of LMW-GS alleles is more abundant than what has been defined by the current nomenclature system that is mainly based on SDS-PAGE system. The MALDI-TOF technology is useful to differentiate these variations. An international joint effort may be needed to assign allele symbols to these newly identified alleles and determine their effects on end-product quality attributes.

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Authors+Show Affiliations

Wang A
State Agriculture Biotechnology Centre, School of Veterinary & Life Sciences, Murdoch University, Australia Export Grains Innovation Centre (AEGIC), Perth, WA, 6150, Australia; Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, Beijing, 100037, China.
Liu L
State Agriculture Biotechnology Centre, School of Veterinary & Life Sciences, Murdoch University, Australia Export Grains Innovation Centre (AEGIC), Perth, WA, 6150, Australia; National Wheat Improvement Centre, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), No. 12 Zhongguancun South Street, Beijing, 100081, China.
Peng Y
State Agriculture Biotechnology Centre, School of Veterinary & Life Sciences, Murdoch University, Australia Export Grains Innovation Centre (AEGIC), Perth, WA, 6150, Australia; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Islam S
State Agriculture Biotechnology Centre, School of Veterinary & Life Sciences, Murdoch University, Australia Export Grains Innovation Centre (AEGIC), Perth, WA, 6150, Australia.
Applebee M
South Australian Research & Development Institute, Waite Campus, 2b Hartley Grove, Urrbrae, SA, 5064, Australia.
Appels R
State Agriculture Biotechnology Centre, School of Veterinary & Life Sciences, Murdoch University, Australia Export Grains Innovation Centre (AEGIC), Perth, WA, 6150, Australia.
Yan Y
Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, Beijing, 100037, China.
Ma W
State Agriculture Biotechnology Centre, School of Veterinary & Life Sciences, Murdoch University, Australia Export Grains Innovation Centre (AEGIC), Perth, WA, 6150, Australia.

MeSH

AllelesGlutensSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26407296

Citation

Wang, Aili, et al. "Identification of Low Molecular Weight Glutenin Alleles By Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF-MS) in Common Wheat (Triticum Aestivum L.)." PloS One, vol. 10, no. 9, 2015, pp. e0138981.
Wang A, Liu L, Peng Y, et al. Identification of Low Molecular Weight Glutenin Alleles by Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF-MS) in Common Wheat (Triticum aestivum L.). PLoS One. 2015;10(9):e0138981.
Wang, A., Liu, L., Peng, Y., Islam, S., Applebee, M., Appels, R., Yan, Y., & Ma, W. (2015). Identification of Low Molecular Weight Glutenin Alleles by Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF-MS) in Common Wheat (Triticum aestivum L.). PloS One, 10(9), e0138981. https://doi.org/10.1371/journal.pone.0138981
Wang A, et al. Identification of Low Molecular Weight Glutenin Alleles By Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF-MS) in Common Wheat (Triticum Aestivum L.). PLoS One. 2015;10(9):e0138981. PubMed PMID: 26407296.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of Low Molecular Weight Glutenin Alleles by Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF-MS) in Common Wheat (Triticum aestivum L.). AU - Wang,Aili, AU - Liu,Li, AU - Peng,Yanchun, AU - Islam,Shahidul, AU - Applebee,Marie, AU - Appels,Rudi, AU - Yan,Yueming, AU - Ma,Wujun, Y1 - 2015/09/25/ PY - 2015/03/27/received PY - 2015/09/08/accepted PY - 2015/9/26/entrez PY - 2015/9/26/pubmed PY - 2016/6/18/medline SP - e0138981 EP - e0138981 JF - PloS one JO - PLoS One VL - 10 IS - 9 N2 - Low molecular weight glutenin subunits (LMW-GS) play an important role in determining dough properties and breadmaking quality. However, resolution of the currently used methodologies for analyzing LMW-GS is rather low which prevents an efficient use of genetic variations associated with these alleles in wheat breeding. The aim of the current study is to evaluate and develop a rapid, simple, and accurate method to differentiate LMW-GS alleles using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A set of standard single LMW-GS allele lines as well as a suite of well documented wheat cultivars were collected from France, CIMMYT, and Canada. Method development and optimization were focused on protein extraction procedures and MALDI-TOF instrument settings to generate reproducible diagnostic spectrum peak profiles for each of the known wheat LMW-GS allele. Results revealed a total of 48 unique allele combinations among the studied genotypes. Characteristic MALDI-TOF peak patterns were obtained for 17 common LMW-GS alleles, including 5 (b, a or c, d, e, f), 7 (a, b, c, d or i, f, g, h) and 5 (a, b, c, d, f) patterns or alleles for the Glu-A3, Glu-B3, and Glu-D3 loci, respectively. In addition, some reproducible MALDI-TOF peak patterns were also obtained that did not match with any known alleles. The results demonstrated a high resolution and throughput nature of MALDI-TOF technology in analyzing LMW-GS alleles, which is suitable for application in wheat breeding programs in processing a large number of wheat lines with high accuracy in limited time. It also suggested that the variation of LMW-GS alleles is more abundant than what has been defined by the current nomenclature system that is mainly based on SDS-PAGE system. The MALDI-TOF technology is useful to differentiate these variations. An international joint effort may be needed to assign allele symbols to these newly identified alleles and determine their effects on end-product quality attributes. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/26407296/Identification_of_Low_Molecular_Weight_Glutenin_Alleles_by_Matrix_Assisted_Laser_Desorption/Ionization_Time_Of_Flight_Mass_Spectrometry__MALDI_TOF_MS__in_Common_Wheat__Triticum_aestivum_L___ DB - PRIME DP - Unbound Medicine ER -