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Characterization and fine mapping of a light-dependent leaf lesion mimic mutant 1 in rice.
Plant Physiol Biochem. 2015 Dec; 97:44-51.PP

Abstract

Plants that spontaneously produce lesion mimics or spots, without any signs of obvious adversity, such as pesticide and mechanical damage, or pathogen infection, are so-called lesion mimic mutants (lmms). In rice, many lmms exhibit enhanced resistance to pathogens, which provides a unique opportunity to uncover the molecular mechanism underlying lmms. We isolated a rice light-dependent leaf lesion mimic mutant 1 (llm1). Lesion spots appeared in the leaves of the llm1 mutant at the tillering stage. Furthermore, the mutant llm1 had similar agronomic traits to wild type rice. Trypan blue and diamiobenzidine staining analyses revealed that the lesion spot formation on the llm1 mutant was due to programmed cell death and reactive oxygen species. The chloroplasts were severely damaged in the llm1 mutant, suggesting that chloroplast damage was associated with the formation of lesion spots in llm1. More importantly, llm1 exhibited enhanced resistance to bacterial blight pathogens within increased expression of pathogenesis related genes (PRs). Using a map-based cloning approach, we delimited the LLM1 locus to a 121-kb interval between two simple sequence repeat markers, RM17470 and RM17473, on chromosome 4. We sequenced the candidate genes on the interval and found that a base mutation had substituted adenine phosphate for thymine in the last exon of LOC_Os04g52130, which led to an amino acid change (Asp(388) to Val) in the llm1 mutant. Our investigation showed that the putative coproporphyrinogen III oxidase (CPOX) encoded by LOC_Os04g52130 was produced by LLM1 and that amino acid Asp(388) was essential for CPOX function. Our study provides the basis for further investigations into the mechanism underlying lesion mimic initiation associated with LLM1.

Authors+Show Affiliations

Rice Research Institute, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu 611130, China; Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University at Wenjiang, Chengdu 611130, China; Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu 611130, China.Rice Research Institute, Sichuan Agricultural University at Wenjiang, Chengdu, Sichuan 611130, China; Key Laboratory of Major Crop Diseases, Sichuan Agricultural University at Wenjiang, Chengdu 611130, China; State Key Laboratory of Hybrid Rice, Sichuan Agricultural University at Wenjiang, Chengdu 611130, China; Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin at Sichuan, Chengdu 611130, China. Electronic address: xwchen88@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26410574

Citation

Wang, Jing, et al. "Characterization and Fine Mapping of a Light-dependent Leaf Lesion Mimic Mutant 1 in Rice." Plant Physiology and Biochemistry : PPB, vol. 97, 2015, pp. 44-51.
Wang J, Ye B, Yin J, et al. Characterization and fine mapping of a light-dependent leaf lesion mimic mutant 1 in rice. Plant Physiol Biochem. 2015;97:44-51.
Wang, J., Ye, B., Yin, J., Yuan, C., Zhou, X., Li, W., He, M., Wang, J., Chen, W., Qin, P., Ma, B., Wang, Y., Li, S., & Chen, X. (2015). Characterization and fine mapping of a light-dependent leaf lesion mimic mutant 1 in rice. Plant Physiology and Biochemistry : PPB, 97, 44-51. https://doi.org/10.1016/j.plaphy.2015.09.001
Wang J, et al. Characterization and Fine Mapping of a Light-dependent Leaf Lesion Mimic Mutant 1 in Rice. Plant Physiol Biochem. 2015;97:44-51. PubMed PMID: 26410574.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization and fine mapping of a light-dependent leaf lesion mimic mutant 1 in rice. AU - Wang,Jing, AU - Ye,Bangquan, AU - Yin,Junjie, AU - Yuan,Can, AU - Zhou,Xiaogang, AU - Li,Weitao, AU - He,Min, AU - Wang,Jichun, AU - Chen,Weilan, AU - Qin,Peng, AU - Ma,Bintian, AU - Wang,Yuping, AU - Li,Shigui, AU - Chen,Xuewei, Y1 - 2015/09/12/ PY - 2015/04/20/received PY - 2015/08/28/revised PY - 2015/09/01/accepted PY - 2015/9/28/entrez PY - 2015/9/28/pubmed PY - 2016/9/7/medline KW - Chloroplast KW - Gene mapping KW - Lesion mimic KW - Rice SP - 44 EP - 51 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 97 N2 - Plants that spontaneously produce lesion mimics or spots, without any signs of obvious adversity, such as pesticide and mechanical damage, or pathogen infection, are so-called lesion mimic mutants (lmms). In rice, many lmms exhibit enhanced resistance to pathogens, which provides a unique opportunity to uncover the molecular mechanism underlying lmms. We isolated a rice light-dependent leaf lesion mimic mutant 1 (llm1). Lesion spots appeared in the leaves of the llm1 mutant at the tillering stage. Furthermore, the mutant llm1 had similar agronomic traits to wild type rice. Trypan blue and diamiobenzidine staining analyses revealed that the lesion spot formation on the llm1 mutant was due to programmed cell death and reactive oxygen species. The chloroplasts were severely damaged in the llm1 mutant, suggesting that chloroplast damage was associated with the formation of lesion spots in llm1. More importantly, llm1 exhibited enhanced resistance to bacterial blight pathogens within increased expression of pathogenesis related genes (PRs). Using a map-based cloning approach, we delimited the LLM1 locus to a 121-kb interval between two simple sequence repeat markers, RM17470 and RM17473, on chromosome 4. We sequenced the candidate genes on the interval and found that a base mutation had substituted adenine phosphate for thymine in the last exon of LOC_Os04g52130, which led to an amino acid change (Asp(388) to Val) in the llm1 mutant. Our investigation showed that the putative coproporphyrinogen III oxidase (CPOX) encoded by LOC_Os04g52130 was produced by LLM1 and that amino acid Asp(388) was essential for CPOX function. Our study provides the basis for further investigations into the mechanism underlying lesion mimic initiation associated with LLM1. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/26410574/Characterization_and_fine_mapping_of_a_light_dependent_leaf_lesion_mimic_mutant_1_in_rice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(15)30103-0 DB - PRIME DP - Unbound Medicine ER -