Tags

Type your tag names separated by a space and hit enter

Protein profile of cotyledon, tegument, and embryonic axis of mature acorns from a non-orthodox plant species: Quercus ilex.
Planta. 2016 Feb; 243(2):369-96.P

Abstract

MAIN CONCLUSION

Contrary to the orthodox seeds, recalcitrant Holm oak seeds possess the enzymatic machinery to start germination during the maturation phase. The protein profile of the different parts, mature seeds, of the Holm oak, a non-orthodox plant species, has been characterized using one- and two-dimensional gel electrophoresis coupled to matrix-assisted laser desorption ionization-time of flight mass spectrometry. Protein content and profiles of the three seed tissues (cotyledon, embryonic axis and tegument) were quite different. The embryonic axis showed 4-fold and 20-fold higher protein content than the cotyledon and the tegument, respectively. Two hundred and twenty-six variable proteins among the three seed parts were identified, being classified according to their function into eight main groups. The cotyledon presented the highest number of metabolic and storage proteins (89% of them are legumin) compared to the embryonic axis and tegument. The embryonic axis had the highest number of the species within the protein fate group. The tegument presented the largest number of the defense-/stress-related and cytoskeleton proteins. This distribution is in good agreement with the biological role of the tissues. The study of the seed tissue proteome demonstrated a compartmentalization of pathways and a division of metabolic tasks between embryonic axis, cotyledon and tegument. This compartmentalization uncovered in our study should provide a starting point for understanding, at the molecular level, the particularities of the recalcitrant seeds.

Authors+Show Affiliations

Laboratory of Extremophile Plants, Biotechnology Centre of Borj Cedria, P. O. Box 901, 2050, Hammam-Lif, Tunisia. sghaierbesma@yahoo.fr. Agroforestry and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Cordoba-CeiA3, Cordoba, Spain. sghaierbesma@yahoo.fr.Agroforestry and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Cordoba-CeiA3, Cordoba, Spain.Agroforestry and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Cordoba-CeiA3, Cordoba, Spain. Department of Chemistry-Biology, Biomedical Sciences Institute, Autonomous University of Ciudad Juárez, Ciudad Juárez, Chihuahua, Mexico.Agroforestry and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Cordoba-CeiA3, Cordoba, Spain.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26424229

Citation

Sghaier-Hammami, Besma, et al. "Protein Profile of Cotyledon, Tegument, and Embryonic Axis of Mature Acorns From a Non-orthodox Plant Species: Quercus Ilex." Planta, vol. 243, no. 2, 2016, pp. 369-96.
Sghaier-Hammami B, Redondo-López I, Valero-Galvàn J, et al. Protein profile of cotyledon, tegument, and embryonic axis of mature acorns from a non-orthodox plant species: Quercus ilex. Planta. 2016;243(2):369-96.
Sghaier-Hammami, B., Redondo-López, I., Valero-Galvàn, J., & Jorrín-Novo, J. V. (2016). Protein profile of cotyledon, tegument, and embryonic axis of mature acorns from a non-orthodox plant species: Quercus ilex. Planta, 243(2), 369-96. https://doi.org/10.1007/s00425-015-2404-3
Sghaier-Hammami B, et al. Protein Profile of Cotyledon, Tegument, and Embryonic Axis of Mature Acorns From a Non-orthodox Plant Species: Quercus Ilex. Planta. 2016;243(2):369-96. PubMed PMID: 26424229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protein profile of cotyledon, tegument, and embryonic axis of mature acorns from a non-orthodox plant species: Quercus ilex. AU - Sghaier-Hammami,Besma, AU - Redondo-López,Inmaculada, AU - Valero-Galvàn,José, AU - Jorrín-Novo,Jesús V, Y1 - 2015/09/30/ PY - 2015/05/07/received PY - 2015/09/03/accepted PY - 2015/10/2/entrez PY - 2015/10/2/pubmed PY - 2016/11/5/medline KW - Different seed parts KW - Holm oak KW - Mature seed KW - Proteomics KW - Recalcitrant SP - 369 EP - 96 JF - Planta JO - Planta VL - 243 IS - 2 N2 - MAIN CONCLUSION: Contrary to the orthodox seeds, recalcitrant Holm oak seeds possess the enzymatic machinery to start germination during the maturation phase. The protein profile of the different parts, mature seeds, of the Holm oak, a non-orthodox plant species, has been characterized using one- and two-dimensional gel electrophoresis coupled to matrix-assisted laser desorption ionization-time of flight mass spectrometry. Protein content and profiles of the three seed tissues (cotyledon, embryonic axis and tegument) were quite different. The embryonic axis showed 4-fold and 20-fold higher protein content than the cotyledon and the tegument, respectively. Two hundred and twenty-six variable proteins among the three seed parts were identified, being classified according to their function into eight main groups. The cotyledon presented the highest number of metabolic and storage proteins (89% of them are legumin) compared to the embryonic axis and tegument. The embryonic axis had the highest number of the species within the protein fate group. The tegument presented the largest number of the defense-/stress-related and cytoskeleton proteins. This distribution is in good agreement with the biological role of the tissues. The study of the seed tissue proteome demonstrated a compartmentalization of pathways and a division of metabolic tasks between embryonic axis, cotyledon and tegument. This compartmentalization uncovered in our study should provide a starting point for understanding, at the molecular level, the particularities of the recalcitrant seeds. SN - 1432-2048 UR - https://www.unboundmedicine.com/medline/citation/26424229/Protein_profile_of_cotyledon_tegument_and_embryonic_axis_of_mature_acorns_from_a_non_orthodox_plant_species:_Quercus_ilex_ L2 - https://dx.doi.org/10.1007/s00425-015-2404-3 DB - PRIME DP - Unbound Medicine ER -