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A knowledge-based molecular screen uncovers a broad-spectrum OsSWEET14 resistance allele to bacterial blight from wild rice.
Plant J. 2015 Nov; 84(4):694-703.PJ

Abstract

Transcription activator-like (TAL) effectors are type III-delivered transcription factors that enhance the virulence of plant pathogenic Xanthomonas species through the activation of host susceptibility (S) genes. TAL effectors recognize their DNA target(s) via a partially degenerate code, whereby modular repeats in the TAL effector bind to nucleotide sequences in the host promoter. Although this knowledge has greatly facilitated our power to identify new S genes, it can also be easily used to screen plant genomes for variations in TAL effector target sequences and to predict for loss-of-function gene candidates in silico. In a proof-of-principle experiment, we screened a germplasm of 169 rice accessions for polymorphism in the promoter of the major bacterial blight susceptibility S gene OsSWEET14, which encodes a sugar transporter targeted by numerous strains of Xanthomonas oryzae pv. oryzae. We identified a single allele with a deletion of 18 bp overlapping with the binding sites targeted by several TAL effectors known to activate the gene. We show that this allele, which we call xa41(t), confers resistance against half of the tested Xoo strains, representative of various geographic origins and genetic lineages, highlighting the selective pressure on the pathogen to accommodate OsSWEET14 polymorphism, and reciprocally the apparent limited possibilities for the host to create variability at this particular S gene. Analysis of xa41(t) conservation across the Oryza genus enabled us to hypothesize scenarios as to its evolutionary history, prior to and during domestication. Our findings demonstrate that resistance through TAL effector-dependent loss of S-gene expression can be greatly fostered upon knowledge-based molecular screening of a large collection of host plants.

Authors+Show Affiliations

UMR IPME, IRD-CIRAD-Université Montpellier 2, Montpellier, France.UMR DIADE IRD/UM2, BP 64501, 34394, Montpellier Cedex 5, France.UMR DIADE IRD/UM2, BP 64501, 34394, Montpellier Cedex 5, France.UMR IPME, IRD-CIRAD-Université Montpellier 2, Montpellier, France.UMR IPME, IRD-CIRAD-Université Montpellier 2, Montpellier, France.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26426417

Citation

Hutin, Mathilde, et al. "A Knowledge-based Molecular Screen Uncovers a Broad-spectrum OsSWEET14 Resistance Allele to Bacterial Blight From Wild Rice." The Plant Journal : for Cell and Molecular Biology, vol. 84, no. 4, 2015, pp. 694-703.
Hutin M, Sabot F, Ghesquière A, et al. A knowledge-based molecular screen uncovers a broad-spectrum OsSWEET14 resistance allele to bacterial blight from wild rice. Plant J. 2015;84(4):694-703.
Hutin, M., Sabot, F., Ghesquière, A., Koebnik, R., & Szurek, B. (2015). A knowledge-based molecular screen uncovers a broad-spectrum OsSWEET14 resistance allele to bacterial blight from wild rice. The Plant Journal : for Cell and Molecular Biology, 84(4), 694-703. https://doi.org/10.1111/tpj.13042
Hutin M, et al. A Knowledge-based Molecular Screen Uncovers a Broad-spectrum OsSWEET14 Resistance Allele to Bacterial Blight From Wild Rice. Plant J. 2015;84(4):694-703. PubMed PMID: 26426417.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A knowledge-based molecular screen uncovers a broad-spectrum OsSWEET14 resistance allele to bacterial blight from wild rice. AU - Hutin,Mathilde, AU - Sabot,François, AU - Ghesquière,Alain, AU - Koebnik,Ralf, AU - Szurek,Boris, PY - 2015/07/01/received PY - 2015/09/01/revised PY - 2015/09/08/accepted PY - 2015/10/2/entrez PY - 2015/10/2/pubmed PY - 2016/12/15/medline KW - Oryza barthii KW - SWEET KW - TAL effector KW - Xanthomonas oryzae KW - susceptibility (S) gene KW - xa41(t) SP - 694 EP - 703 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 84 IS - 4 N2 - Transcription activator-like (TAL) effectors are type III-delivered transcription factors that enhance the virulence of plant pathogenic Xanthomonas species through the activation of host susceptibility (S) genes. TAL effectors recognize their DNA target(s) via a partially degenerate code, whereby modular repeats in the TAL effector bind to nucleotide sequences in the host promoter. Although this knowledge has greatly facilitated our power to identify new S genes, it can also be easily used to screen plant genomes for variations in TAL effector target sequences and to predict for loss-of-function gene candidates in silico. In a proof-of-principle experiment, we screened a germplasm of 169 rice accessions for polymorphism in the promoter of the major bacterial blight susceptibility S gene OsSWEET14, which encodes a sugar transporter targeted by numerous strains of Xanthomonas oryzae pv. oryzae. We identified a single allele with a deletion of 18 bp overlapping with the binding sites targeted by several TAL effectors known to activate the gene. We show that this allele, which we call xa41(t), confers resistance against half of the tested Xoo strains, representative of various geographic origins and genetic lineages, highlighting the selective pressure on the pathogen to accommodate OsSWEET14 polymorphism, and reciprocally the apparent limited possibilities for the host to create variability at this particular S gene. Analysis of xa41(t) conservation across the Oryza genus enabled us to hypothesize scenarios as to its evolutionary history, prior to and during domestication. Our findings demonstrate that resistance through TAL effector-dependent loss of S-gene expression can be greatly fostered upon knowledge-based molecular screening of a large collection of host plants. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/26426417/A_knowledge_based_molecular_screen_uncovers_a_broad_spectrum_OsSWEET14_resistance_allele_to_bacterial_blight_from_wild_rice_ L2 - https://doi.org/10.1111/tpj.13042 DB - PRIME DP - Unbound Medicine ER -