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Feeding on resistant rice leads to enhanced expression of defender against apoptotic cell death (OoDAD1) in the Asian rice gall midge.
BMC Plant Biol. 2015 Oct 01; 15:235.BP

Abstract

BACKGROUND

The Asian rice gall midge (Orseolia oryzae) is a destructive insect pest of rice. Gall midge infestation in rice triggers either compatible or incompatible interactions leading to survival or mortality of the feeding maggots, respectively. In incompatible interactions, generation of plant allelochemicals/defense molecules and/or inability of the maggots to continue feeding on the host initiate(s) apoptosis within the maggots. Unraveling these molecular events, triggered within the maggots as a response to feeding on resistant hosts, will enable us to obtain a better understanding of host resistance. The present study points towards the likely involvement of a defender against apoptotic cell death gene (DAD1) in the insect in response to the host defense.

RESULTS

The cDNA coding for the DAD1 orthologue in the rice gall midge (OoDAD1) consisted of 339 nucleotides with one intron of 85 bp and two exons of 208 and 131 nucleotides. The deduced amino acid sequence of OoDAD1 showed a high degree of homology (94.6%) with DAD1 orthologue from the Hessian fly (Mayetiola destructor)--a major dipteran pest of wheat. Southern hybridization analysis indicated that OoDAD1 was present as a single copy in the genomes of the Asian rice gall midge biotypes (GMB) 1, 4 and 4 M. In the interactions involving GMB4 with Jaya (susceptible rice host) the expression level of OoDAD1 in feeding maggots gradually increased to 3-fold at 96 hai (hours after infestation) and peaked to 3.5-fold at 96 hai when compared to that at 24 hai. In contrast, expression in maggots feeding on RP2068 (resistant host) showed a steep increase of more than 8-fold at 24 hai and this level was sustained at 48, 72 and 96 hai when compared with the level in maggots feeding on Jaya at 24 hai. Recombinant OoDAD1, expressed in E. coli cells, when injected into rice seedlings induced a hypersensitive response (HR) in the resistant rice host, RP2068, but not in the susceptible rice variety, Jaya.

CONCLUSIONS

The results indicate that the expression of OoDAD1 is triggered in the feeding maggots probably due to the host resistance response and therefore, is likely an important molecule in the initial stages of the interaction between the midge and its rice host.

Authors+Show Affiliations

Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110 067, India. deepak22sinha@yahoo.co.in.Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110 067, India. isha@icgeb.res.in.Directorate of Rice Research, Rajendranagar, Hyderabad, 500 030, India. jbentur@yahoo.com. Agri Biotech Foundation, Rajendranagar, Hyderabad, 500 030, India. jbentur@yahoo.com.Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110 067, India. suresh@icgeb.res.in.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26428861

Citation

Sinha, Deepak K., et al. "Feeding On Resistant Rice Leads to Enhanced Expression of Defender Against Apoptotic Cell Death (OoDAD1) in the Asian Rice Gall Midge." BMC Plant Biology, vol. 15, 2015, p. 235.
Sinha DK, Atray I, Bentur JS, et al. Feeding on resistant rice leads to enhanced expression of defender against apoptotic cell death (OoDAD1) in the Asian rice gall midge. BMC Plant Biol. 2015;15:235.
Sinha, D. K., Atray, I., Bentur, J. S., & Nair, S. (2015). Feeding on resistant rice leads to enhanced expression of defender against apoptotic cell death (OoDAD1) in the Asian rice gall midge. BMC Plant Biology, 15, 235. https://doi.org/10.1186/s12870-015-0618-y
Sinha DK, et al. Feeding On Resistant Rice Leads to Enhanced Expression of Defender Against Apoptotic Cell Death (OoDAD1) in the Asian Rice Gall Midge. BMC Plant Biol. 2015 Oct 1;15:235. PubMed PMID: 26428861.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Feeding on resistant rice leads to enhanced expression of defender against apoptotic cell death (OoDAD1) in the Asian rice gall midge. AU - Sinha,Deepak K, AU - Atray,Isha, AU - Bentur,J S, AU - Nair,Suresh, Y1 - 2015/10/01/ PY - 2015/03/24/received PY - 2015/09/16/accepted PY - 2015/10/3/entrez PY - 2015/10/3/pubmed PY - 2016/7/7/medline SP - 235 EP - 235 JF - BMC plant biology JO - BMC Plant Biol VL - 15 N2 - BACKGROUND: The Asian rice gall midge (Orseolia oryzae) is a destructive insect pest of rice. Gall midge infestation in rice triggers either compatible or incompatible interactions leading to survival or mortality of the feeding maggots, respectively. In incompatible interactions, generation of plant allelochemicals/defense molecules and/or inability of the maggots to continue feeding on the host initiate(s) apoptosis within the maggots. Unraveling these molecular events, triggered within the maggots as a response to feeding on resistant hosts, will enable us to obtain a better understanding of host resistance. The present study points towards the likely involvement of a defender against apoptotic cell death gene (DAD1) in the insect in response to the host defense. RESULTS: The cDNA coding for the DAD1 orthologue in the rice gall midge (OoDAD1) consisted of 339 nucleotides with one intron of 85 bp and two exons of 208 and 131 nucleotides. The deduced amino acid sequence of OoDAD1 showed a high degree of homology (94.6%) with DAD1 orthologue from the Hessian fly (Mayetiola destructor)--a major dipteran pest of wheat. Southern hybridization analysis indicated that OoDAD1 was present as a single copy in the genomes of the Asian rice gall midge biotypes (GMB) 1, 4 and 4 M. In the interactions involving GMB4 with Jaya (susceptible rice host) the expression level of OoDAD1 in feeding maggots gradually increased to 3-fold at 96 hai (hours after infestation) and peaked to 3.5-fold at 96 hai when compared to that at 24 hai. In contrast, expression in maggots feeding on RP2068 (resistant host) showed a steep increase of more than 8-fold at 24 hai and this level was sustained at 48, 72 and 96 hai when compared with the level in maggots feeding on Jaya at 24 hai. Recombinant OoDAD1, expressed in E. coli cells, when injected into rice seedlings induced a hypersensitive response (HR) in the resistant rice host, RP2068, but not in the susceptible rice variety, Jaya. CONCLUSIONS: The results indicate that the expression of OoDAD1 is triggered in the feeding maggots probably due to the host resistance response and therefore, is likely an important molecule in the initial stages of the interaction between the midge and its rice host. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/26428861/Feeding_on_resistant_rice_leads_to_enhanced_expression_of_defender_against_apoptotic_cell_death__OoDAD1__in_the_Asian_rice_gall_midge_ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-015-0618-y DB - PRIME DP - Unbound Medicine ER -