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Angelica sinensis polysaccharides promotes apoptosis in human breast cancer cells via CREB-regulated caspase-3 activation.
Biochem Biophys Res Commun. 2015 Nov 20; 467(3):562-9.BB

Abstract

Angelica sinensis polysaccharide (ASP) is purified from the fresh roots of Angelica sinensis (AS). This traditional Chinese medicine has been used for thousands of years for treating gynecological diseases and used in functional foods for the prevention and treatment of various diseases, such as inflammation and cancer. The antitumor activity of ASP is related to its biological activities, because it suppresses a variety of pro-proliferative or anti-apoptotic factors that are dramatically expressed in cancer cells of given types. In this study, we show that angelica sinensis polysaccharide induced apoptosis in breast cancer cells of T47D over-expressing the Cyclic AMP response element binding protein (CREB), inducing apoptosis-related signaling pathway activity. The result also found that ASP caused cell death was linked to caspase activity, accompanied by the loss of mitochondrial membrane potential, cytochrome c release, and Bax translocation from the cytosol to the mitochondria. We found that ASP significantly affected the poly-ADP-ribose polymerase (PARP), Bcl-2 Associated X Protein (Bax), Bcl-2, Bcl-xL and apoptotic protease activating facter-1 (Apaf1) protein expression in a dose- and time-dependent manner. DAPI staining and Flow cytometry were used to analyze apoptosis. The nude mice xenograft model was used to evaluate the antitumor effect of ASP in vivo. ASP has profound antitumor effect on T47D cells, probably by inducing apoptosis through CREB signaling pathway. Thus, these results suggest that ASP would be a promising therapeutic agent for breast cancer.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Zhou WJ
Department of Breast and Thyroid Surgery, Huaihe Hospital, Henan University, Kaifeng 475000, China.
Wang S
Department of Breast and Thyroid Surgery, Huaihe Hospital, Henan University, Kaifeng 475000, China.
Hu Z
Department of Breast and Thyroid Surgery, Huaihe Hospital, Henan University, Kaifeng 475000, China; Zhengzhou Center for Disease Control and Prevention, Zhengzhou 475000, China. Electronic address: zhuanghu475000@sina.com.
Zhou ZY
Department of Breast and Thyroid Surgery, Huaihe Hospital, Henan University, Kaifeng 475000, China; Zhengzhou Center for Disease Control and Prevention, Zhengzhou 475000, China.
Song CJ
Department of Breast and Thyroid Surgery, Huaihe Hospital, Henan University, Kaifeng 475000, China; Zhengzhou Center for Disease Control and Prevention, Zhengzhou 475000, China.

MeSH

Angelica sinensisAnimalsApoptosisBreast NeoplasmsCaspase 3Cell Line, TumorCyclic AMP Response Element-Binding ProteinEnzyme ActivationFemaleHumansMiceMice, NudeMitochondriaPolysaccharidesXenograft Model Antitumor Assays

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26431878

Citation

Zhou, Wei-Jie, et al. "Angelica Sinensis Polysaccharides Promotes Apoptosis in Human Breast Cancer Cells Via CREB-regulated Caspase-3 Activation." Biochemical and Biophysical Research Communications, vol. 467, no. 3, 2015, pp. 562-9.
Zhou WJ, Wang S, Hu Z, et al. Angelica sinensis polysaccharides promotes apoptosis in human breast cancer cells via CREB-regulated caspase-3 activation. Biochem Biophys Res Commun. 2015;467(3):562-9.
Zhou, W. J., Wang, S., Hu, Z., Zhou, Z. Y., & Song, C. J. (2015). Angelica sinensis polysaccharides promotes apoptosis in human breast cancer cells via CREB-regulated caspase-3 activation. Biochemical and Biophysical Research Communications, 467(3), 562-9. https://doi.org/10.1016/j.bbrc.2015.09.145
Zhou WJ, et al. Angelica Sinensis Polysaccharides Promotes Apoptosis in Human Breast Cancer Cells Via CREB-regulated Caspase-3 Activation. Biochem Biophys Res Commun. 2015 Nov 20;467(3):562-9. PubMed PMID: 26431878.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Angelica sinensis polysaccharides promotes apoptosis in human breast cancer cells via CREB-regulated caspase-3 activation. AU - Zhou,Wei-Jie, AU - Wang,Sheng, AU - Hu,Zhuang, AU - Zhou,Zhen-Yu, AU - Song,Cai-Juan, Y1 - 2015/09/30/ PY - 2015/09/22/received PY - 2015/09/26/accepted PY - 2015/10/4/entrez PY - 2015/10/4/pubmed PY - 2016/2/19/medline KW - Angelica sinensis polysaccharide KW - Breast cancer KW - CREB KW - Caspases-3 KW - T47D SP - 562 EP - 9 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 467 IS - 3 N2 - Angelica sinensis polysaccharide (ASP) is purified from the fresh roots of Angelica sinensis (AS). This traditional Chinese medicine has been used for thousands of years for treating gynecological diseases and used in functional foods for the prevention and treatment of various diseases, such as inflammation and cancer. The antitumor activity of ASP is related to its biological activities, because it suppresses a variety of pro-proliferative or anti-apoptotic factors that are dramatically expressed in cancer cells of given types. In this study, we show that angelica sinensis polysaccharide induced apoptosis in breast cancer cells of T47D over-expressing the Cyclic AMP response element binding protein (CREB), inducing apoptosis-related signaling pathway activity. The result also found that ASP caused cell death was linked to caspase activity, accompanied by the loss of mitochondrial membrane potential, cytochrome c release, and Bax translocation from the cytosol to the mitochondria. We found that ASP significantly affected the poly-ADP-ribose polymerase (PARP), Bcl-2 Associated X Protein (Bax), Bcl-2, Bcl-xL and apoptotic protease activating facter-1 (Apaf1) protein expression in a dose- and time-dependent manner. DAPI staining and Flow cytometry were used to analyze apoptosis. The nude mice xenograft model was used to evaluate the antitumor effect of ASP in vivo. ASP has profound antitumor effect on T47D cells, probably by inducing apoptosis through CREB signaling pathway. Thus, these results suggest that ASP would be a promising therapeutic agent for breast cancer. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/26431878/Angelica_sinensis_polysaccharides_promotes_apoptosis_in_human_breast_cancer_cells_via_CREB_regulated_caspase_3_activation_ DB - PRIME DP - Unbound Medicine ER -
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