Tags

Type your tag names separated by a space and hit enter

Isolation and characterization of a virus infecting the freshwater algae Chrysochromulina parva.
Virology. 2015 Dec; 486:105-15.V

Abstract

Water samples from Lake Ontario, Canada were tested for lytic activity against the freshwater haptophyte algae Chrysochromulina parva. A filterable lytic agent was isolated and identified as a virus via transmission electron microscopy and molecular methods. The virus, CpV-BQ1, is icosahedral, ca. 145nm in diameter, assembled within the cytoplasm, and has a genome size of ca. 485kb. Sequences obtained through PCR-amplification of DNA polymerase (polB) genes clustered among sequences from the family Phycodnaviridae, whereas major capsid protein (MCP) sequences clustered among sequences from either the Phycodnaviridae or Mimiviridae. Based on quantitative molecular assays, C. parva׳s abundance in Lake Ontario was relatively stable, yet CpV-BQ1׳s abundance was variable suggesting complex virus-host dynamics. This study demonstrates that CpV-BQ1 is a member of the proposed order Megavirales with characteristics of both phycodnaviruses and mimiviruses indicating that, in addition to its complex ecological dynamics, it also has a complex evolutionary history.

Authors+Show Affiliations

Department of Biology, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga, Ontario, Canada L5L 1C6.Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks Street, Toronto, Ontario, Canada M5S 3B2.Department of Biology, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga, Ontario, Canada L5L 1C6.Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks Street, Toronto, Ontario, Canada M5S 3B2.Department of Biology, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga, Ontario, Canada L5L 1C6.Department of Biology, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga, Ontario, Canada L5L 1C6; Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks Street, Toronto, Ontario, Canada M5S 3B2. Electronic address: steven.short@utoronto.ca.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26432023

Citation

Mirza, S F., et al. "Isolation and Characterization of a Virus Infecting the Freshwater Algae Chrysochromulina Parva." Virology, vol. 486, 2015, pp. 105-15.
Mirza SF, Staniewski MA, Short CM, et al. Isolation and characterization of a virus infecting the freshwater algae Chrysochromulina parva. Virology. 2015;486:105-15.
Mirza, S. F., Staniewski, M. A., Short, C. M., Long, A. M., Chaban, Y. V., & Short, S. M. (2015). Isolation and characterization of a virus infecting the freshwater algae Chrysochromulina parva. Virology, 486, 105-15. https://doi.org/10.1016/j.virol.2015.09.005
Mirza SF, et al. Isolation and Characterization of a Virus Infecting the Freshwater Algae Chrysochromulina Parva. Virology. 2015;486:105-15. PubMed PMID: 26432023.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and characterization of a virus infecting the freshwater algae Chrysochromulina parva. AU - Mirza,S F, AU - Staniewski,M A, AU - Short,C M, AU - Long,A M, AU - Chaban,Y V, AU - Short,S M, Y1 - 2015/09/30/ PY - 2015/08/04/received PY - 2015/08/26/revised PY - 2015/09/08/accepted PY - 2015/10/4/entrez PY - 2015/10/4/pubmed PY - 2016/6/10/medline KW - Algal virus KW - Haptophyta KW - Mimiviridae KW - NCLDV KW - Phycodnaviridae KW - Prymnesiovirus SP - 105 EP - 15 JF - Virology JO - Virology VL - 486 N2 - Water samples from Lake Ontario, Canada were tested for lytic activity against the freshwater haptophyte algae Chrysochromulina parva. A filterable lytic agent was isolated and identified as a virus via transmission electron microscopy and molecular methods. The virus, CpV-BQ1, is icosahedral, ca. 145nm in diameter, assembled within the cytoplasm, and has a genome size of ca. 485kb. Sequences obtained through PCR-amplification of DNA polymerase (polB) genes clustered among sequences from the family Phycodnaviridae, whereas major capsid protein (MCP) sequences clustered among sequences from either the Phycodnaviridae or Mimiviridae. Based on quantitative molecular assays, C. parva׳s abundance in Lake Ontario was relatively stable, yet CpV-BQ1׳s abundance was variable suggesting complex virus-host dynamics. This study demonstrates that CpV-BQ1 is a member of the proposed order Megavirales with characteristics of both phycodnaviruses and mimiviruses indicating that, in addition to its complex ecological dynamics, it also has a complex evolutionary history. SN - 1096-0341 UR - https://www.unboundmedicine.com/medline/citation/26432023/Isolation_and_characterization_of_a_virus_infecting_the_freshwater_algae_Chrysochromulina_parva_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(15)00396-7 DB - PRIME DP - Unbound Medicine ER -