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Dietary pantothenic acid depressed the gill immune and physical barrier function via NF-κB, TOR, Nrf2, p38MAPK and MLCK signaling pathways in grass carp (Ctenopharyngodon idella).
Fish Shellfish Immunol. 2015 Nov; 47(1):500-10.FS

Abstract

This study explored the effects of pantothenic acid (PA) on the immune and physical barrier function, and relative mRNA levels of signaling molecules in the gill of grass carp (Ctenopharyngodon idella). The results indicated that compared with optimal PA supplementation, PA deficiency (1.31 mg/kg diet) decreased gill interleukin 10, transforming growth factor β1, inhibitor of κBα (IκBα), eIF4E-binding protein 2, Claudin b and ZO-1 mRNA levels; anti-superoxide anion activity, and activities and mRNA levels of copper/zinc superoxide dismutase, manganese superoxide dismutase, glutathione peroxidase, glutathione reductase and NF-E2-related factor (P < 0.05). Additionally, PA deficiency and excess (75.08 mg/kg diet) decreased gill complement 3 and glutathione contents, lysozyme and acid phosphatase, anti-hydroxy radical, catalase and glutathione S-transferases activities, and liver-expression antimicrobial peptide 2, hepcidin, Claudin 3, Claudin c and Occludin mRNA levels (P < 0.05). Conversely, PA deficiency increased gill reactive oxygen species and protein carbonyl contents, and interferon γ2, interleukin 8, nuclear factor kappa B P65, Claudin 15a, Kelch-like ECH-associating protein 1a and Kelch-like ECH-associating protein 1b mRNA levels (P<0.05). Moreover, PA deficiency and excess increased gill malondialdehyde content, and tumor necrosis factor α, interleukin 1β, IκB kinase α, IκB kinase β, IκB kinase γ, target of rapamycin and ribosomal S6 protein kinase1 p38 mitogen-activated protein kinases and myosin light-chain kinase mRNA levels (P<0.05). In conclusion, PA deficiency decreased immune and physical barrier function, and regulated relative mRNA levels of signaling molecules in fish gill. Based on the quadratic regression analysis of gill lysozyme activity, the optimal PA levels in grass carp (253.44-745.25 g) were estimated to be 36.97 mg/kg diet.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Li L
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Feng L
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Fish Nutrition and Safety Production University Key Laboratory of Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Jiang WD
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Fish Nutrition and Safety Production University Key Laboratory of Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Jiang J
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Fish Nutrition and Safety Production University Key Laboratory of Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Wu P
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Fish Nutrition and Safety Production University Key Laboratory of Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Zhao J
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Kuang SY
Animal Nutrition Institute, Sichuan Academy of Animal Science, Chengdu, 610066, Sichuan, China.
Tang L
Animal Nutrition Institute, Sichuan Academy of Animal Science, Chengdu, 610066, Sichuan, China.
Tang WN
Animal Nutrition Institute, Sichuan Academy of Animal Science, Chengdu, 610066, Sichuan, China.
Zhang YA
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Zhou XQ
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Fish Nutrition and Safety Production University Key Laboratory of Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Electronic address: zhouxq@sicau.edu.cn.
Liu Y
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Fish Nutrition and Safety Production University Key Laboratory of Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Electronic address: kyckgk@hotmail.com.

MeSH

Animal FeedAnimalsCarpsDietDietary SupplementsFish ProteinsGillsImmunity, InnatePantothenic AcidRNA, Messenger

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26432048

Citation

Li, Li, et al. "Dietary Pantothenic Acid Depressed the Gill Immune and Physical Barrier Function Via NF-κB, TOR, Nrf2, p38MAPK and MLCK Signaling Pathways in Grass Carp (Ctenopharyngodon Idella)." Fish & Shellfish Immunology, vol. 47, no. 1, 2015, pp. 500-10.
Li L, Feng L, Jiang WD, et al. Dietary pantothenic acid depressed the gill immune and physical barrier function via NF-κB, TOR, Nrf2, p38MAPK and MLCK signaling pathways in grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol. 2015;47(1):500-10.
Li, L., Feng, L., Jiang, W. D., Jiang, J., Wu, P., Zhao, J., Kuang, S. Y., Tang, L., Tang, W. N., Zhang, Y. A., Zhou, X. Q., & Liu, Y. (2015). Dietary pantothenic acid depressed the gill immune and physical barrier function via NF-κB, TOR, Nrf2, p38MAPK and MLCK signaling pathways in grass carp (Ctenopharyngodon idella). Fish & Shellfish Immunology, 47(1), 500-10. https://doi.org/10.1016/j.fsi.2015.09.038
Li L, et al. Dietary Pantothenic Acid Depressed the Gill Immune and Physical Barrier Function Via NF-κB, TOR, Nrf2, p38MAPK and MLCK Signaling Pathways in Grass Carp (Ctenopharyngodon Idella). Fish Shellfish Immunol. 2015;47(1):500-10. PubMed PMID: 26432048.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary pantothenic acid depressed the gill immune and physical barrier function via NF-κB, TOR, Nrf2, p38MAPK and MLCK signaling pathways in grass carp (Ctenopharyngodon idella). AU - Li,Li, AU - Feng,Lin, AU - Jiang,Wei-Dan, AU - Jiang,Jun, AU - Wu,Pei, AU - Zhao,Juan, AU - Kuang,Sheng-Yao, AU - Tang,Ling, AU - Tang,Wu-Neng, AU - Zhang,Yong-An, AU - Zhou,Xiao-Qiu, AU - Liu,Yang, Y1 - 2015/09/30/ PY - 2015/06/05/received PY - 2015/08/04/revised PY - 2015/09/24/accepted PY - 2015/10/4/entrez PY - 2015/10/4/pubmed PY - 2016/8/11/medline KW - Antioxidant enzyme KW - Gill KW - Grass carp KW - Immune KW - Pantothenic acid KW - Physical barrier KW - Tight junction protein KW - mRNA level SP - 500 EP - 10 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 47 IS - 1 N2 - This study explored the effects of pantothenic acid (PA) on the immune and physical barrier function, and relative mRNA levels of signaling molecules in the gill of grass carp (Ctenopharyngodon idella). The results indicated that compared with optimal PA supplementation, PA deficiency (1.31 mg/kg diet) decreased gill interleukin 10, transforming growth factor β1, inhibitor of κBα (IκBα), eIF4E-binding protein 2, Claudin b and ZO-1 mRNA levels; anti-superoxide anion activity, and activities and mRNA levels of copper/zinc superoxide dismutase, manganese superoxide dismutase, glutathione peroxidase, glutathione reductase and NF-E2-related factor (P < 0.05). Additionally, PA deficiency and excess (75.08 mg/kg diet) decreased gill complement 3 and glutathione contents, lysozyme and acid phosphatase, anti-hydroxy radical, catalase and glutathione S-transferases activities, and liver-expression antimicrobial peptide 2, hepcidin, Claudin 3, Claudin c and Occludin mRNA levels (P < 0.05). Conversely, PA deficiency increased gill reactive oxygen species and protein carbonyl contents, and interferon γ2, interleukin 8, nuclear factor kappa B P65, Claudin 15a, Kelch-like ECH-associating protein 1a and Kelch-like ECH-associating protein 1b mRNA levels (P<0.05). Moreover, PA deficiency and excess increased gill malondialdehyde content, and tumor necrosis factor α, interleukin 1β, IκB kinase α, IκB kinase β, IκB kinase γ, target of rapamycin and ribosomal S6 protein kinase1 p38 mitogen-activated protein kinases and myosin light-chain kinase mRNA levels (P<0.05). In conclusion, PA deficiency decreased immune and physical barrier function, and regulated relative mRNA levels of signaling molecules in fish gill. Based on the quadratic regression analysis of gill lysozyme activity, the optimal PA levels in grass carp (253.44-745.25 g) were estimated to be 36.97 mg/kg diet. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/26432048/Dietary_pantothenic_acid_depressed_the_gill_immune_and_physical_barrier_function_via_NF_κB_TOR_Nrf2_p38MAPK_and_MLCK_signaling_pathways_in_grass_carp__Ctenopharyngodon_idella__ DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓5]

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