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7-prenyloxi-6-methoxycoumarin from Polygala sabulosa A.W. Bennett Regulates p38 MAPK and NF-kB Pathways Inhibiting the Inflammation Induced by Carrageenan in the Mouse Model of Pleurisy.
Inflamm Allergy Drug Targets. 2015; 14(1):37-46.IA

Abstract

CONTEXT

Polygala sabulosa, popularly known as "timutu-pinheirinho," has been used in Brazilian folk medicine for the treatment of bowel and kidney disorders and as an expectorant.

OBJECTIVE

Evaluate the anti-inflammatory effects of the crude extract (CE), acetonic fraction (Ac), and the main compound, 7-prenyloxi-6-methoxycoumarin (PC) on a mouse model of carrageenan-induced pleurisy.

MATERIALS AND METHODS

A mouse model of carrageenan-induced pleurisy was used to investigate the effects of P. sabulosa CE, Ac and PC on leukocyte migration, exudate formation, activities of myeloperoxidase (MPO), and adenosine-deaminase (ADA), levels of tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β) and nitric oxide (NO). In addition, the effect of the plant material on lung histology was also evaluated. The effects of PC on the TNF-α, IL-1β and NO synthase 2 (NOS2) mRNA expression, were also investigated. Finally, the effect of PC on the nuclear factor-kappa B (NF-κB) and p38 mitogen-activated protein kinase (p38 MAPK) was also evaluated.

RESULTS

CE, Ac and PC reduced inflammation in the pleural cavity and lungs. This effect was evidenced by reduction on all inflammatory parameters evaluated; the exception being the inability of the CE to inhibit exudate formation. In isolation, PC showed reduction on mRNA levels of TNF-α, IL-1β and NOS2, and on activation of the NF-κB and p38 MAPK pathways.

CONCLUSION

The presented results show that P. sabulosa has significant anti-inflammatory activity, as does its main compound, PC. Moreover, the results suggest that PC exerts its effects mainly by inhibited the NF-κB and p38 MAPK pathways.

Authors+Show Affiliations

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableDepartment of Clinical Analyses, Centre of Health Sciences, Federal University of Santa Catarina, Campus Universitário - Trindade, 88040-970, Florianopolis, SC, Brazil. eduardo.dalmarco@ufsc.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26434700

Citation

Dos Santos Nascimento, Marcus Vinicius Pereira, et al. "7-prenyloxi-6-methoxycoumarin From Polygala Sabulosa A.W. Bennett Regulates P38 MAPK and NF-kB Pathways Inhibiting the Inflammation Induced By Carrageenan in the Mouse Model of Pleurisy." Inflammation & Allergy Drug Targets, vol. 14, no. 1, 2015, pp. 37-46.
Dos Santos Nascimento MV, Arruda-Silva F, Luz AB, et al. 7-prenyloxi-6-methoxycoumarin from Polygala sabulosa A.W. Bennett Regulates p38 MAPK and NF-kB Pathways Inhibiting the Inflammation Induced by Carrageenan in the Mouse Model of Pleurisy. Inflamm Allergy Drug Targets. 2015;14(1):37-46.
Dos Santos Nascimento, M. V., Arruda-Silva, F., Luz, A. B., Venzke, D., Queiroz, G. S., Mendes, B. G., Fernandes-Ribeiro, E. R., Fröde, T. S., Pizzolatti, M. G., & Dalmarco, E. M. (2015). 7-prenyloxi-6-methoxycoumarin from Polygala sabulosa A.W. Bennett Regulates p38 MAPK and NF-kB Pathways Inhibiting the Inflammation Induced by Carrageenan in the Mouse Model of Pleurisy. Inflammation & Allergy Drug Targets, 14(1), 37-46.
Dos Santos Nascimento MV, et al. 7-prenyloxi-6-methoxycoumarin From Polygala Sabulosa A.W. Bennett Regulates P38 MAPK and NF-kB Pathways Inhibiting the Inflammation Induced By Carrageenan in the Mouse Model of Pleurisy. Inflamm Allergy Drug Targets. 2015;14(1):37-46. PubMed PMID: 26434700.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 7-prenyloxi-6-methoxycoumarin from Polygala sabulosa A.W. Bennett Regulates p38 MAPK and NF-kB Pathways Inhibiting the Inflammation Induced by Carrageenan in the Mouse Model of Pleurisy. AU - Dos Santos Nascimento,Marcus Vinicius Pereira, AU - Arruda-Silva,Fábio, AU - Luz,Ana Beatriz G, AU - Venzke,Dalila, AU - Queiroz,Gustavo S, AU - Mendes,Beatriz G, AU - Fernandes-Ribeiro,Eduardo R, AU - Fröde,Tânia S, AU - Pizzolatti,Moacir G, AU - Dalmarco,Eduardo M, PY - 2015/08/19/received PY - 2015/10/02/revised PY - 2015/10/05/accepted PY - 2015/10/6/entrez PY - 2015/10/6/pubmed PY - 2016/10/1/medline SP - 37 EP - 46 JF - Inflammation & allergy drug targets JO - Inflamm Allergy Drug Targets VL - 14 IS - 1 N2 - CONTEXT: Polygala sabulosa, popularly known as "timutu-pinheirinho," has been used in Brazilian folk medicine for the treatment of bowel and kidney disorders and as an expectorant. OBJECTIVE: Evaluate the anti-inflammatory effects of the crude extract (CE), acetonic fraction (Ac), and the main compound, 7-prenyloxi-6-methoxycoumarin (PC) on a mouse model of carrageenan-induced pleurisy. MATERIALS AND METHODS: A mouse model of carrageenan-induced pleurisy was used to investigate the effects of P. sabulosa CE, Ac and PC on leukocyte migration, exudate formation, activities of myeloperoxidase (MPO), and adenosine-deaminase (ADA), levels of tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β) and nitric oxide (NO). In addition, the effect of the plant material on lung histology was also evaluated. The effects of PC on the TNF-α, IL-1β and NO synthase 2 (NOS2) mRNA expression, were also investigated. Finally, the effect of PC on the nuclear factor-kappa B (NF-κB) and p38 mitogen-activated protein kinase (p38 MAPK) was also evaluated. RESULTS: CE, Ac and PC reduced inflammation in the pleural cavity and lungs. This effect was evidenced by reduction on all inflammatory parameters evaluated; the exception being the inability of the CE to inhibit exudate formation. In isolation, PC showed reduction on mRNA levels of TNF-α, IL-1β and NOS2, and on activation of the NF-κB and p38 MAPK pathways. CONCLUSION: The presented results show that P. sabulosa has significant anti-inflammatory activity, as does its main compound, PC. Moreover, the results suggest that PC exerts its effects mainly by inhibited the NF-κB and p38 MAPK pathways. SN - 2212-4055 UR - https://www.unboundmedicine.com/medline/citation/26434700/7_prenyloxi_6_methoxycoumarin_from_Polygala_sabulosa_A_W__Bennett_Regulates_p38_MAPK_and_NF_kB_Pathways_Inhibiting_the_Inflammation_Induced_by_Carrageenan_in_the_Mouse_Model_of_Pleurisy_ L2 - http://www.eurekaselect.com/135507/article DB - PRIME DP - Unbound Medicine ER -