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Detection of Candida spp. in the vagina of a cohort of nulliparous pregnant women by culture and molecular methods: Is there an association between maternal vaginal and infant oral colonisation?
Aust N Z J Obstet Gynaecol. 2016 Apr; 56(2):179-84.AN

Abstract

BACKGROUND

Most studies describing vaginal Candida spp. in pregnancy focus on symptomatic vaginitis, rather than asymptomatic colonisation, and solely utilise microbiological culture. The extent to which asymptomatic vaginal carriage may represent a reservoir for infant oral colonisation has been highly debated.

MATERIALS AND METHODS

This study formed part of the Candida and Staphylococcus Transmission Longitudinal Evaluation (CASTLE) study, in Melbourne, Australia, from 2009 to 2011 and used culture and molecular methods to examine vaginal swabs collected late in the third trimester of pregnancy for Candida spp. Oral swabs from infants were also examined using culture methods.

RESULTS

Overall, 80 of 356 (22%) women were positive for Candida spp; the majority being Candida albicans (83%). Candida glabrata and other Candida spp. were also identified, but in much lower numbers. Molecular analysis identified numerous positive samples not detected by culture, including 13 cases of C. albicans. In addition, some positive samples only recorded to genus level by culture were accurately identified as either C. albicans or C. glabrata following molecular analyses. Eighteen infants recorded positive Candida spp. cultures, predominantly C. albicans. However, there were only four (25%) mother/infant dyads where C. albicans was detected.

CONCLUSIONS

This study provides valuable data on asymptomatic colonisation rates of Candida spp. within an asymptomatic population of women late in pregnancy. The utilisation of molecular methods improved the rate of detection and provided a more accurate means for identification of non-albicans Candida spp. The low mother/infant colonisation rate suggests that non-maternal sources are likely involved in determining infant oral colonisation status.

Authors+Show Affiliations

Judith Lumley Centre (formerly Mother & Child Health Research), La Trobe University, Melbourne, Victoria, Australia. Women's Centre for Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia. School of Women's and Infants' Health, University of Western Australia, Subiaco, Western Australia, Australia.Judith Lumley Centre (formerly Mother & Child Health Research), La Trobe University, Melbourne, Victoria, Australia.Women's Centre for Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia.Women's Centre for Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia. Department of Obstetrics and Gynaecology, University of Melbourne, Parkville, Victoria, Australia.Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia. Department of Paediatrics, University of Melbourne, Parkville, Victoria, Australia.Deakin Population Health, Deakin University, Burwood, Victoria, Australia.Judith Lumley Centre (formerly Mother & Child Health Research), La Trobe University, Melbourne, Victoria, Australia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26437337

Citation

Payne, Matthew S., et al. "Detection of Candida Spp. in the Vagina of a Cohort of Nulliparous Pregnant Women By Culture and Molecular Methods: Is There an Association Between Maternal Vaginal and Infant Oral Colonisation?" The Australian & New Zealand Journal of Obstetrics & Gynaecology, vol. 56, no. 2, 2016, pp. 179-84.
Payne MS, Cullinane M, Garland SM, et al. Detection of Candida spp. in the vagina of a cohort of nulliparous pregnant women by culture and molecular methods: Is there an association between maternal vaginal and infant oral colonisation? Aust N Z J Obstet Gynaecol. 2016;56(2):179-84.
Payne, M. S., Cullinane, M., Garland, S. M., Tabrizi, S. N., Donath, S. M., Bennett, C. M., & Amir, L. H. (2016). Detection of Candida spp. in the vagina of a cohort of nulliparous pregnant women by culture and molecular methods: Is there an association between maternal vaginal and infant oral colonisation? The Australian & New Zealand Journal of Obstetrics & Gynaecology, 56(2), 179-84. https://doi.org/10.1111/ajo.12409
Payne MS, et al. Detection of Candida Spp. in the Vagina of a Cohort of Nulliparous Pregnant Women By Culture and Molecular Methods: Is There an Association Between Maternal Vaginal and Infant Oral Colonisation. Aust N Z J Obstet Gynaecol. 2016;56(2):179-84. PubMed PMID: 26437337.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Candida spp. in the vagina of a cohort of nulliparous pregnant women by culture and molecular methods: Is there an association between maternal vaginal and infant oral colonisation? AU - Payne,Matthew S, AU - Cullinane,Meabh, AU - Garland,Suzanne M, AU - Tabrizi,Sepehr N, AU - Donath,Susan M, AU - Bennett,Catherine M, AU - Amir,Lisa H, Y1 - 2015/10/05/ PY - 2015/03/10/received PY - 2015/08/31/accepted PY - 2015/10/6/entrez PY - 2015/10/6/pubmed PY - 2017/1/12/medline KW - CHROMagar KW - Candida spp. KW - infant KW - real-time PCR KW - vagina SP - 179 EP - 84 JF - The Australian & New Zealand journal of obstetrics & gynaecology JO - Aust N Z J Obstet Gynaecol VL - 56 IS - 2 N2 - BACKGROUND: Most studies describing vaginal Candida spp. in pregnancy focus on symptomatic vaginitis, rather than asymptomatic colonisation, and solely utilise microbiological culture. The extent to which asymptomatic vaginal carriage may represent a reservoir for infant oral colonisation has been highly debated. MATERIALS AND METHODS: This study formed part of the Candida and Staphylococcus Transmission Longitudinal Evaluation (CASTLE) study, in Melbourne, Australia, from 2009 to 2011 and used culture and molecular methods to examine vaginal swabs collected late in the third trimester of pregnancy for Candida spp. Oral swabs from infants were also examined using culture methods. RESULTS: Overall, 80 of 356 (22%) women were positive for Candida spp; the majority being Candida albicans (83%). Candida glabrata and other Candida spp. were also identified, but in much lower numbers. Molecular analysis identified numerous positive samples not detected by culture, including 13 cases of C. albicans. In addition, some positive samples only recorded to genus level by culture were accurately identified as either C. albicans or C. glabrata following molecular analyses. Eighteen infants recorded positive Candida spp. cultures, predominantly C. albicans. However, there were only four (25%) mother/infant dyads where C. albicans was detected. CONCLUSIONS: This study provides valuable data on asymptomatic colonisation rates of Candida spp. within an asymptomatic population of women late in pregnancy. The utilisation of molecular methods improved the rate of detection and provided a more accurate means for identification of non-albicans Candida spp. The low mother/infant colonisation rate suggests that non-maternal sources are likely involved in determining infant oral colonisation status. SN - 1479-828X UR - https://www.unboundmedicine.com/medline/citation/26437337/Detection_of_Candida_spp__in_the_vagina_of_a_cohort_of_nulliparous_pregnant_women_by_culture_and_molecular_methods:_Is_there_an_association_between_maternal_vaginal_and_infant_oral_colonisation DB - PRIME DP - Unbound Medicine ER -