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Comparative Studies on Phenolic Composition, Antioxidant, Wound Healing and Cytotoxic Activities of Selected Achillea L. Species Growing in Turkey.
Molecules 2015; 20(10):17976-8000M

Abstract

Turkey is one of the most important centers of diversity for the genus Achillea L. in the world. Keeping in mind the immense medicinal importance of phenols, in this study, three species growing in Turkey, A. coarctata Poir. (AC), A. kotschyi Boiss. subsp. kotschyi (AK) and A. lycaonica Boiss. & Heldr. (AL) were evaluated for their phenolic compositions, total phenolic contents (TPC), antioxidant properties, wound healing potencies on NIH-3T3 fibroblasts and cytotoxic effects on MCF-7 human breast cancer cells. Comprehensive LC-MS/MS analysis revealed that AK was distinctively rich in chlorogenic acid, hyperoside, apigenin, hesperidin, rutin, kaempferol and luteolin (2890.6, 987.3, 797.0, 422.5, 188.1, 159.4 and 121.2 µg analyte/g extract, respectively). The findings exhibited a strong correlation between TPC and both free radical scavenging activity and total antioxidant capacity (TAC). Among studied species, the highest TPC (148.00 mg GAE/g extract) and TAC (2.080 UAE), the strongest radical scavenging (EC50 = 32.63 μg/mL), the most prominent wound healing and most abundant cytotoxic activities were observed with AK. The results suggested that AK is a valuable source of flavonoids and chlorogenic acid with important antioxidant, wound healing and cytotoxic activities. These findings warrant further studies to assess the potential of AK as a bioactive source that could be exploited in pharmaceutical, cosmetics and food industries.

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    Authors+Show Affiliations

    Agar OT
    Department of Pharmaceutical Botany, Faculty of Pharmacy, Hacettepe University, Ankara 06100, Turkey. tagar@hacettepe.edu.tr.
    Dikmen M
    Department of Pharmacology, Faculty of Pharmacy, Anadolu University, Eskisehir 26470, Turkey. mirisd@anadolu.edu.tr.
    Ozturk N
    Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskisehir 26470, Turkey. nozturk@anadolu.edu.tr.
    Yilmaz MA
    Dicle University Science and Technology Research and Application Center, Diyarbakır 21280, Turkey. mustafaabdullahyilmaz@gmail.com.
    Temel H
    Dicle University Science and Technology Research and Application Center, Diyarbakır 21280, Turkey. htemel@dicle.edu.tr. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Dicle University, Diyarbakir 21280, Turkey. htemel@dicle.edu.tr.
    Turkmenoglu FP
    Department of Pharmaceutical Botany, Faculty of Pharmacy, Hacettepe University, Ankara 06100, Turkey. fpt@hacettepe.edu.tr.

    MeSH

    AchilleaAnimalsAntioxidantsCell LineCell ProliferationChromatography, LiquidCollagenFibroblastsHumansMicePhenolsPlant ExtractsTandem Mass SpectrometryTurkeyWound Healing

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    26437391

    Citation

    Agar, Osman Tuncay, et al. "Comparative Studies On Phenolic Composition, Antioxidant, Wound Healing and Cytotoxic Activities of Selected Achillea L. Species Growing in Turkey." Molecules (Basel, Switzerland), vol. 20, no. 10, 2015, pp. 17976-8000.
    Agar OT, Dikmen M, Ozturk N, et al. Comparative Studies on Phenolic Composition, Antioxidant, Wound Healing and Cytotoxic Activities of Selected Achillea L. Species Growing in Turkey. Molecules. 2015;20(10):17976-8000.
    Agar, O. T., Dikmen, M., Ozturk, N., Yilmaz, M. A., Temel, H., & Turkmenoglu, F. P. (2015). Comparative Studies on Phenolic Composition, Antioxidant, Wound Healing and Cytotoxic Activities of Selected Achillea L. Species Growing in Turkey. Molecules (Basel, Switzerland), 20(10), pp. 17976-8000. doi:10.3390/molecules201017976.
    Agar OT, et al. Comparative Studies On Phenolic Composition, Antioxidant, Wound Healing and Cytotoxic Activities of Selected Achillea L. Species Growing in Turkey. Molecules. 2015 Sep 30;20(10):17976-8000. PubMed PMID: 26437391.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Comparative Studies on Phenolic Composition, Antioxidant, Wound Healing and Cytotoxic Activities of Selected Achillea L. Species Growing in Turkey. AU - Agar,Osman Tuncay, AU - Dikmen,Miris, AU - Ozturk,Nilgun, AU - Yilmaz,Mustafa Abdullah, AU - Temel,Hamdi, AU - Turkmenoglu,Fatma Pinar, Y1 - 2015/09/30/ PY - 2015/02/28/received PY - 2015/09/20/revised PY - 2015/09/21/accepted PY - 2015/10/6/entrez PY - 2015/10/6/pubmed PY - 2016/6/28/medline KW - Achillea KW - LC-MS/MS KW - antioxidant KW - breast cancer KW - cytotoxic KW - natural product KW - phenolics KW - wound healing SP - 17976 EP - 8000 JF - Molecules (Basel, Switzerland) JO - Molecules VL - 20 IS - 10 N2 - Turkey is one of the most important centers of diversity for the genus Achillea L. in the world. Keeping in mind the immense medicinal importance of phenols, in this study, three species growing in Turkey, A. coarctata Poir. (AC), A. kotschyi Boiss. subsp. kotschyi (AK) and A. lycaonica Boiss. & Heldr. (AL) were evaluated for their phenolic compositions, total phenolic contents (TPC), antioxidant properties, wound healing potencies on NIH-3T3 fibroblasts and cytotoxic effects on MCF-7 human breast cancer cells. Comprehensive LC-MS/MS analysis revealed that AK was distinctively rich in chlorogenic acid, hyperoside, apigenin, hesperidin, rutin, kaempferol and luteolin (2890.6, 987.3, 797.0, 422.5, 188.1, 159.4 and 121.2 µg analyte/g extract, respectively). The findings exhibited a strong correlation between TPC and both free radical scavenging activity and total antioxidant capacity (TAC). Among studied species, the highest TPC (148.00 mg GAE/g extract) and TAC (2.080 UAE), the strongest radical scavenging (EC50 = 32.63 μg/mL), the most prominent wound healing and most abundant cytotoxic activities were observed with AK. The results suggested that AK is a valuable source of flavonoids and chlorogenic acid with important antioxidant, wound healing and cytotoxic activities. These findings warrant further studies to assess the potential of AK as a bioactive source that could be exploited in pharmaceutical, cosmetics and food industries. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/26437391/Comparative_Studies_on_Phenolic_Composition_Antioxidant_Wound_Healing_and_Cytotoxic_Activities_of_Selected_Achillea_L__Species_Growing_in_Turkey_ L2 - http://www.mdpi.com/resolver?pii=molecules201017976 DB - PRIME DP - Unbound Medicine ER -