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Identification of H2S3 and H2S produced by 3-mercaptopyruvate sulfurtransferase in the brain.
Sci Rep. 2015 Oct 06; 5:14774.SR

Abstract

Hydrogen polysulfides (H2Sn) have a higher number of sulfane sulfur atoms than hydrogen sulfide (H2S), which has various physiological roles. We recently found H2Sn in the brain. H2Sn induced some responses previously attributed to H2S but with much greater potency than H2S. However, the number of sulfur atoms in H2Sn and its producing enzyme were unknown. Here, we detected H2S3 and H2S, which were produced from 3-mercaptopyruvate (3 MP) by 3-mercaptopyruvate sulfurtransferase (3MST), in the brain. High performance liquid chromatography with fluorescence detection (LC-FL) and tandem mass spectrometry (LC-MS/MS) analyses showed that H2S3 and H2S were produced from 3 MP in the brain cells of wild-type mice but not 3MST knockout (3MST-KO) mice. Purified recombinant 3MST and lysates of COS cells expressing 3MST produced H2S3 from 3 MP, while those expressing defective 3MST mutants did not. H2S3 was localized in the cytosol of cells. H2S3 was also produced from H2S by 3MST and rhodanese. H2S2 was identified as a minor H2Sn, and 3 MP did not affect the H2S5 level. The present study provides new insights into the physiology of H2S3 and H2S, as well as novel therapeutic targets for diseases in which these molecules are involved.

Authors+Show Affiliations

Department of Molecular Pharmacology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.Department of Molecular Pharmacology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.Department of Molecular Pharmacology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan. Department of Analytical Biochemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.Department of Molecular Pharmacology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.Radioisotope Center, Nippon Medical School, 1-1-5 Sendagi, Bunkyo, Tokyo 113-8602, Japan.Department of Pharmacology and Experimental Therapeutics and Cardiovascular Center of Excellence, LSU Health Science Center, New Orleans, LA 70112, USA.Department of Analytical Biochemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.Department of Molecular Pharmacology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26437775

Citation

Kimura, Yuka, et al. "Identification of H2S3 and H2S Produced By 3-mercaptopyruvate Sulfurtransferase in the Brain." Scientific Reports, vol. 5, 2015, p. 14774.
Kimura Y, Toyofuku Y, Koike S, et al. Identification of H2S3 and H2S produced by 3-mercaptopyruvate sulfurtransferase in the brain. Sci Rep. 2015;5:14774.
Kimura, Y., Toyofuku, Y., Koike, S., Shibuya, N., Nagahara, N., Lefer, D., Ogasawara, Y., & Kimura, H. (2015). Identification of H2S3 and H2S produced by 3-mercaptopyruvate sulfurtransferase in the brain. Scientific Reports, 5, 14774. https://doi.org/10.1038/srep14774
Kimura Y, et al. Identification of H2S3 and H2S Produced By 3-mercaptopyruvate Sulfurtransferase in the Brain. Sci Rep. 2015 Oct 6;5:14774. PubMed PMID: 26437775.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of H2S3 and H2S produced by 3-mercaptopyruvate sulfurtransferase in the brain. AU - Kimura,Yuka, AU - Toyofuku,Yukiko, AU - Koike,Shin, AU - Shibuya,Norihiro, AU - Nagahara,Noriyuki, AU - Lefer,David, AU - Ogasawara,Yuki, AU - Kimura,Hideo, Y1 - 2015/10/06/ PY - 2015/06/14/received PY - 2015/09/09/accepted PY - 2015/10/7/entrez PY - 2015/10/7/pubmed PY - 2016/8/9/medline SP - 14774 EP - 14774 JF - Scientific reports JO - Sci Rep VL - 5 N2 - Hydrogen polysulfides (H2Sn) have a higher number of sulfane sulfur atoms than hydrogen sulfide (H2S), which has various physiological roles. We recently found H2Sn in the brain. H2Sn induced some responses previously attributed to H2S but with much greater potency than H2S. However, the number of sulfur atoms in H2Sn and its producing enzyme were unknown. Here, we detected H2S3 and H2S, which were produced from 3-mercaptopyruvate (3 MP) by 3-mercaptopyruvate sulfurtransferase (3MST), in the brain. High performance liquid chromatography with fluorescence detection (LC-FL) and tandem mass spectrometry (LC-MS/MS) analyses showed that H2S3 and H2S were produced from 3 MP in the brain cells of wild-type mice but not 3MST knockout (3MST-KO) mice. Purified recombinant 3MST and lysates of COS cells expressing 3MST produced H2S3 from 3 MP, while those expressing defective 3MST mutants did not. H2S3 was localized in the cytosol of cells. H2S3 was also produced from H2S by 3MST and rhodanese. H2S2 was identified as a minor H2Sn, and 3 MP did not affect the H2S5 level. The present study provides new insights into the physiology of H2S3 and H2S, as well as novel therapeutic targets for diseases in which these molecules are involved. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/26437775/Identification_of_H2S3_and_H2S_produced_by_3_mercaptopyruvate_sulfurtransferase_in_the_brain_ L2 - https://doi.org/10.1038/srep14774 DB - PRIME DP - Unbound Medicine ER -