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Increased ethanol production by deletion of HAP4 in recombinant xylose-assimilating Saccharomyces cerevisiae.
J Ind Microbiol Biotechnol. 2015 Dec; 42(12):1623-31.JI

Abstract

The Saccharomyces cerevisiae HAP4 gene encodes a transcription activator that plays a key role in controlling the expression of genes involved in mitochondrial respiration and reductive pathways. This work examines the effect of knockout of the HAP4 gene on aerobic ethanol production in a xylose-utilizing S. cerevisiae strain. A hap4-deleted recombinant yeast strain (B42-DHAP4) showed increased maximum concentration, production rate, and yield of ethanol compared with the reference strain MA-B42, irrespective of cultivation medium (glucose, xylose, or glucose/xylose mixtures). Notably, B42-DHAP4 was capable of producing ethanol from xylose as the sole carbon source under aerobic conditions, whereas no ethanol was produced by MA-B42. Moreover, the rate of ethanol production and ethanol yield (0.44 g/g) from the detoxified hydrolysate of wood chips was markedly improved in B42-DHAP4 compared to MA-B42. Thus, the results of this study support the view that deleting HAP4 in xylose-utilizing S. cerevisiae strains represents a useful strategy in ethanol production processes.

Authors+Show Affiliations

Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-0046, Japan. a-matsushika@aist.go.jp.Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-0046, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26438430

Citation

Matsushika, Akinori, and Tamotsu Hoshino. "Increased Ethanol Production By Deletion of HAP4 in Recombinant Xylose-assimilating Saccharomyces Cerevisiae." Journal of Industrial Microbiology & Biotechnology, vol. 42, no. 12, 2015, pp. 1623-31.
Matsushika A, Hoshino T. Increased ethanol production by deletion of HAP4 in recombinant xylose-assimilating Saccharomyces cerevisiae. J Ind Microbiol Biotechnol. 2015;42(12):1623-31.
Matsushika, A., & Hoshino, T. (2015). Increased ethanol production by deletion of HAP4 in recombinant xylose-assimilating Saccharomyces cerevisiae. Journal of Industrial Microbiology & Biotechnology, 42(12), 1623-31. https://doi.org/10.1007/s10295-015-1695-5
Matsushika A, Hoshino T. Increased Ethanol Production By Deletion of HAP4 in Recombinant Xylose-assimilating Saccharomyces Cerevisiae. J Ind Microbiol Biotechnol. 2015;42(12):1623-31. PubMed PMID: 26438430.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased ethanol production by deletion of HAP4 in recombinant xylose-assimilating Saccharomyces cerevisiae. AU - Matsushika,Akinori, AU - Hoshino,Tamotsu, Y1 - 2015/10/05/ PY - 2015/07/01/received PY - 2015/09/25/accepted PY - 2015/10/7/entrez PY - 2015/10/7/pubmed PY - 2016/5/3/medline KW - Ethanol production KW - HAP4 gene KW - Lignocellulosic hydrolysate KW - Saccharomyces cerevisiae KW - Xylose SP - 1623 EP - 31 JF - Journal of industrial microbiology & biotechnology JO - J Ind Microbiol Biotechnol VL - 42 IS - 12 N2 - The Saccharomyces cerevisiae HAP4 gene encodes a transcription activator that plays a key role in controlling the expression of genes involved in mitochondrial respiration and reductive pathways. This work examines the effect of knockout of the HAP4 gene on aerobic ethanol production in a xylose-utilizing S. cerevisiae strain. A hap4-deleted recombinant yeast strain (B42-DHAP4) showed increased maximum concentration, production rate, and yield of ethanol compared with the reference strain MA-B42, irrespective of cultivation medium (glucose, xylose, or glucose/xylose mixtures). Notably, B42-DHAP4 was capable of producing ethanol from xylose as the sole carbon source under aerobic conditions, whereas no ethanol was produced by MA-B42. Moreover, the rate of ethanol production and ethanol yield (0.44 g/g) from the detoxified hydrolysate of wood chips was markedly improved in B42-DHAP4 compared to MA-B42. Thus, the results of this study support the view that deleting HAP4 in xylose-utilizing S. cerevisiae strains represents a useful strategy in ethanol production processes. SN - 1476-5535 UR - https://www.unboundmedicine.com/medline/citation/26438430/Increased_ethanol_production_by_deletion_of_HAP4_in_recombinant_xylose_assimilating_Saccharomyces_cerevisiae_ DB - PRIME DP - Unbound Medicine ER -