Tags

Type your tag names separated by a space and hit enter

Avirulence gene mapping in the Hessian fly (Mayetiola destructor) reveals a protein phosphatase 2C effector gene family.
J Insect Physiol. 2016 Jan; 84:22-31.JI

Abstract

The genetic tractability of the Hessian fly (HF, Mayetiola destructor) provides an opportunity to investigate the mechanisms insects use to induce plant gall formation. Here we demonstrate that capacity using the newly sequenced HF genome by identifying the gene (vH24) that elicits effector-triggered immunity in wheat (Triticum spp.) seedlings carrying HF resistance gene H24. vH24 was mapped within a 230-kb genomic fragment near the telomere of HF chromosome X1. That fragment contains only 21 putative genes. The best candidate vH24 gene in this region encodes a protein containing a secretion signal and a type-2 serine/threonine protein phosphatase (PP2C) domain. This gene has an H24-virulence associated insertion in its promoter that appears to silence transcription of the gene in H24-virulent larvae. Candidate vH24 is a member of a small family of genes that encode secretion signals and PP2C domains. It belongs to the fraction of genes in the HF genome previously predicted to encode effector proteins. Because PP2C proteins are not normally secreted, our results suggest that these are PP2C effectors that HF larvae inject into wheat cells to redirect, or interfere, with wheat signal transduction pathways.

Authors+Show Affiliations

Department of Entomology, Purdue University, West Lafayette, IN 47907, United States. Electronic address: zhao.1369@osu.edu.USDA-ARS and Department of Entomology, Purdue University, West Lafayette, IN 47907, United States. Electronic address: shukle@purdue.edu.Department of Entomology, Purdue University, West Lafayette, IN 47907, United States. Electronic address: navarroe@purdue.edu.USDA-ARS and Department of Entomology, Kansas State University, Manhattan, KS 66506, United States. Electronic address: ming-shun.chen@ars.usda.gov.Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX 77030, United States. Electronic address: stephenr@bcm.edu.Department of Entomology, Purdue University, West Lafayette, IN 47907, United States. Electronic address: stuartjj@purdue.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

26439791

Citation

Zhao, Chaoyang, et al. "Avirulence Gene Mapping in the Hessian Fly (Mayetiola Destructor) Reveals a Protein Phosphatase 2C Effector Gene Family." Journal of Insect Physiology, vol. 84, 2016, pp. 22-31.
Zhao C, Shukle R, Navarro-Escalante L, et al. Avirulence gene mapping in the Hessian fly (Mayetiola destructor) reveals a protein phosphatase 2C effector gene family. J Insect Physiol. 2016;84:22-31.
Zhao, C., Shukle, R., Navarro-Escalante, L., Chen, M., Richards, S., & Stuart, J. J. (2016). Avirulence gene mapping in the Hessian fly (Mayetiola destructor) reveals a protein phosphatase 2C effector gene family. Journal of Insect Physiology, 84, 22-31. https://doi.org/10.1016/j.jinsphys.2015.10.001
Zhao C, et al. Avirulence Gene Mapping in the Hessian Fly (Mayetiola Destructor) Reveals a Protein Phosphatase 2C Effector Gene Family. J Insect Physiol. 2016;84:22-31. PubMed PMID: 26439791.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Avirulence gene mapping in the Hessian fly (Mayetiola destructor) reveals a protein phosphatase 2C effector gene family. AU - Zhao,Chaoyang, AU - Shukle,Richard, AU - Navarro-Escalante,Lucio, AU - Chen,Mingshun, AU - Richards,Stephen, AU - Stuart,Jeffrey J, Y1 - 2015/10/09/ PY - 2015/03/30/received PY - 2015/09/28/revised PY - 2015/10/01/accepted PY - 2015/10/7/entrez PY - 2015/10/7/pubmed PY - 2016/9/10/medline KW - Avirulence gene KW - Effector protein KW - Gene-for-gene interaction KW - Plant resistance KW - Plant-gall KW - Protein phosphatase SP - 22 EP - 31 JF - Journal of insect physiology JO - J Insect Physiol VL - 84 N2 - The genetic tractability of the Hessian fly (HF, Mayetiola destructor) provides an opportunity to investigate the mechanisms insects use to induce plant gall formation. Here we demonstrate that capacity using the newly sequenced HF genome by identifying the gene (vH24) that elicits effector-triggered immunity in wheat (Triticum spp.) seedlings carrying HF resistance gene H24. vH24 was mapped within a 230-kb genomic fragment near the telomere of HF chromosome X1. That fragment contains only 21 putative genes. The best candidate vH24 gene in this region encodes a protein containing a secretion signal and a type-2 serine/threonine protein phosphatase (PP2C) domain. This gene has an H24-virulence associated insertion in its promoter that appears to silence transcription of the gene in H24-virulent larvae. Candidate vH24 is a member of a small family of genes that encode secretion signals and PP2C domains. It belongs to the fraction of genes in the HF genome previously predicted to encode effector proteins. Because PP2C proteins are not normally secreted, our results suggest that these are PP2C effectors that HF larvae inject into wheat cells to redirect, or interfere, with wheat signal transduction pathways. SN - 1879-1611 UR - https://www.unboundmedicine.com/medline/citation/26439791/Avirulence_gene_mapping_in_the_Hessian_fly__Mayetiola_destructor__reveals_a_protein_phosphatase_2C_effector_gene_family_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-1910(15)00194-8 DB - PRIME DP - Unbound Medicine ER -