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Solid-phase sample extraction for rapid determination of methylmalonic acid in serum and urine by a stable-isotope-dilution method.
Clin Chem. 1989 Feb; 35(2):260-4.CC

Abstract

Methylmalonic acid concentrations are increased in serum in vitamin B12 (cobalamin) deficiency. Here I demonstrate the successful use of anion-exchange extraction for improving newly developed analytical procedures and describe well-documented, reliable performance of this method for rapid determination of methylmalonic acid. The sorbent counter ion is formate, and the elution solvent is formic acid. The dicyclohexyl derivative is measured by selected ion monitoring. For serum, the assay curve is linear from 0.026 to 200 mumol/L. The normal reference interval is 0.08 to 0.56 mumol/L. Added methylmalonic acid is accurately quantified. The sensitivity and the precision exceed those of the current method by three orders of magnitude. The total and within-day CVs are 4.6% to 7.9% and 2.6% to 4.7%, respectively. Similar figures were obtained for urine. This convenient method is useful for evaluation of cobalamin deficiency, especially in patients with normal or moderately depressed cobalamin concentrations in serum.

Authors+Show Affiliations

Rasmussen K
University Department of Clinical Chemistry, Aarhus Kommunehospital, Denmark.

MeSH

AdultGas Chromatography-Mass SpectrometryHumansIndicator Dilution TechniquesMalonatesMethylmalonic AcidVitamin B 12 Deficiency

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2644059

Citation

Rasmussen, K. "Solid-phase Sample Extraction for Rapid Determination of Methylmalonic Acid in Serum and Urine By a Stable-isotope-dilution Method." Clinical Chemistry, vol. 35, no. 2, 1989, pp. 260-4.
Rasmussen K. Solid-phase sample extraction for rapid determination of methylmalonic acid in serum and urine by a stable-isotope-dilution method. Clin Chem. 1989;35(2):260-4.
Rasmussen, K. (1989). Solid-phase sample extraction for rapid determination of methylmalonic acid in serum and urine by a stable-isotope-dilution method. Clinical Chemistry, 35(2), 260-4.
Rasmussen K. Solid-phase Sample Extraction for Rapid Determination of Methylmalonic Acid in Serum and Urine By a Stable-isotope-dilution Method. Clin Chem. 1989;35(2):260-4. PubMed PMID: 2644059.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Solid-phase sample extraction for rapid determination of methylmalonic acid in serum and urine by a stable-isotope-dilution method. A1 - Rasmussen,K, PY - 1989/2/1/pubmed PY - 1989/2/1/medline PY - 1989/2/1/entrez SP - 260 EP - 4 JF - Clinical chemistry JO - Clin Chem VL - 35 IS - 2 N2 - Methylmalonic acid concentrations are increased in serum in vitamin B12 (cobalamin) deficiency. Here I demonstrate the successful use of anion-exchange extraction for improving newly developed analytical procedures and describe well-documented, reliable performance of this method for rapid determination of methylmalonic acid. The sorbent counter ion is formate, and the elution solvent is formic acid. The dicyclohexyl derivative is measured by selected ion monitoring. For serum, the assay curve is linear from 0.026 to 200 mumol/L. The normal reference interval is 0.08 to 0.56 mumol/L. Added methylmalonic acid is accurately quantified. The sensitivity and the precision exceed those of the current method by three orders of magnitude. The total and within-day CVs are 4.6% to 7.9% and 2.6% to 4.7%, respectively. Similar figures were obtained for urine. This convenient method is useful for evaluation of cobalamin deficiency, especially in patients with normal or moderately depressed cobalamin concentrations in serum. SN - 0009-9147 UR - https://www.unboundmedicine.com/medline/citation/2644059/Solid_phase_sample_extraction_for_rapid_determination_of_methylmalonic_acid_in_serum_and_urine_by_a_stable_isotope_dilution_method_ DB - PRIME DP - Unbound Medicine ER -