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Importance of methodological standardization for the ektacytometric measures of red blood cell deformability in sickle cell anemia.
Clin Hemorheol Microcirc. 2016; 62(2):173-9.CH

Abstract

Red blood cell (RBC) deformability is severely decreased in patients with sickle cell anemia (SCA), which plays a role in the pathophysiology of the disease. However, investigation of RBC deformability from SCA patients demands careful methodological considerations. We assessed RBC deformability by ektacytometry (LORRCA MaxSis, Mechatronics, The Netherlands) in 6 healthy individuals and 49 SCA patients and tested the effects of different heights of the RBC diffraction patterns, obtained by altering the camera gain of the LORRCA, on the result of RBC deformability measurements, expressed as Elongation Index (EI). Results indicate that the pattern of RBCs from control subjects adopts an elliptical shape under shear stress, whereas the pattern of RBCs from individuals with SCA adopts a diamond shape arising from the superposition of elliptical and circular patterns. The latter represent rigid RBCs. While the EI measures did not change with the variations of the RBC diffraction pattern heights in the control subjects, we observed a decrease of EI when the RBC diffraction pattern height is increased in the SCA group. The differences in SCA EI values measured at 5 Pa between the different diffraction pattern heights correlated with the percent of hemoglobin S and the percent of sickled RBC observed by microscopy. Our study confirms that the camera gain or aperture of the ektacytometer should be used to standardize the size of the RBC diffraction pattern height when measuring RBC deformability in sickle cell patients and underscores the potential clinical utility of this technique.

Authors+Show Affiliations

Laboratoire CRIS EA647, Team "Vascular Biology and Red Blood Cell", Université Lyon 1, Université de Lyon, Lyon, France. Laboratoire de Biochimie et de Biologie Moléculatire, Hôpital Edouard Herriot, Lyon, France. Laboratoired'Excellence GR-Ex « The red cell: from genesis to death », Paris, France.National Institutes of Health, Washington, DC, USA.Laboratoire CRIS EA647, Team "Vascular Biology and Red Blood Cell", Université Lyon 1, Université de Lyon, Lyon, France. Laboratoired'Excellence GR-Ex « The red cell: from genesis to death », Paris, France.Laboratoire CRIS EA647, Team "Vascular Biology and Red Blood Cell", Université Lyon 1, Université de Lyon, Lyon, France. Laboratoire de Biochimie et de Biologie Moléculatire, Hôpital Edouard Herriot, Lyon, France. Laboratoired'Excellence GR-Ex « The red cell: from genesis to death », Paris, France.Academic Medical Center, Amsterdam, The Netherlands.National Institutes of Health, Washington, DC, USA.National Institutes of Health, Washington, DC, USA.Institut d'Hématologie et d'Oncologie Pédiatrique, Lyon, France.Institut d'Hématologie et d'Oncologie Pédiatrique, Lyon, France.Institut d'Hématologie et d'Oncologie Pédiatrique, Lyon, France.Institut d'Hématologie et d'Oncologie Pédiatrique, Lyon, France.Service de Médecine Interne, Hôpital Edouard Herriot, Lyon, France.Laboratoire CRIS EA647, Team "Vascular Biology and Red Blood Cell", Université Lyon 1, Université de Lyon, Lyon, France. Laboratoired'Excellence GR-Ex « The red cell: from genesis to death », Paris, France.Laboratoire CRIS EA647, Team "Vascular Biology and Red Blood Cell", Université Lyon 1, Université de Lyon, Lyon, France. Laboratoired'Excellence GR-Ex « The red cell: from genesis to death », Paris, France. Institut Universitaire de France, Paris, France.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26444610

Citation

Renoux, Céline, et al. "Importance of Methodological Standardization for the Ektacytometric Measures of Red Blood Cell Deformability in Sickle Cell Anemia." Clinical Hemorheology and Microcirculation, vol. 62, no. 2, 2016, pp. 173-9.
Renoux C, Parrow N, Faes C, et al. Importance of methodological standardization for the ektacytometric measures of red blood cell deformability in sickle cell anemia. Clin Hemorheol Microcirc. 2016;62(2):173-9.
Renoux, C., Parrow, N., Faes, C., Joly, P., Hardeman, M., Tisdale, J., Levine, M., Garnier, N., Bertrand, Y., Kebaili, K., Cuzzubbo, D., Cannas, G., Martin, C., & Connes, P. (2016). Importance of methodological standardization for the ektacytometric measures of red blood cell deformability in sickle cell anemia. Clinical Hemorheology and Microcirculation, 62(2), 173-9. https://doi.org/10.3233/CH-151979
Renoux C, et al. Importance of Methodological Standardization for the Ektacytometric Measures of Red Blood Cell Deformability in Sickle Cell Anemia. Clin Hemorheol Microcirc. 2016;62(2):173-9. PubMed PMID: 26444610.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Importance of methodological standardization for the ektacytometric measures of red blood cell deformability in sickle cell anemia. AU - Renoux,Céline, AU - Parrow,Nermi, AU - Faes,Camille, AU - Joly,Philippe, AU - Hardeman,Max, AU - Tisdale,John, AU - Levine,Mark, AU - Garnier,Nathalie, AU - Bertrand,Yves, AU - Kebaili,Kamila, AU - Cuzzubbo,Daniela, AU - Cannas,Giovanna, AU - Martin,Cyril, AU - Connes,Philippe, PY - 2015/10/8/entrez PY - 2015/10/8/pubmed PY - 2016/12/15/medline KW - Ektacytometry KW - clinical relevance KW - red blood cell deformability KW - sickle cell disease KW - standardization SP - 173 EP - 9 JF - Clinical hemorheology and microcirculation JO - Clin Hemorheol Microcirc VL - 62 IS - 2 N2 - Red blood cell (RBC) deformability is severely decreased in patients with sickle cell anemia (SCA), which plays a role in the pathophysiology of the disease. However, investigation of RBC deformability from SCA patients demands careful methodological considerations. We assessed RBC deformability by ektacytometry (LORRCA MaxSis, Mechatronics, The Netherlands) in 6 healthy individuals and 49 SCA patients and tested the effects of different heights of the RBC diffraction patterns, obtained by altering the camera gain of the LORRCA, on the result of RBC deformability measurements, expressed as Elongation Index (EI). Results indicate that the pattern of RBCs from control subjects adopts an elliptical shape under shear stress, whereas the pattern of RBCs from individuals with SCA adopts a diamond shape arising from the superposition of elliptical and circular patterns. The latter represent rigid RBCs. While the EI measures did not change with the variations of the RBC diffraction pattern heights in the control subjects, we observed a decrease of EI when the RBC diffraction pattern height is increased in the SCA group. The differences in SCA EI values measured at 5 Pa between the different diffraction pattern heights correlated with the percent of hemoglobin S and the percent of sickled RBC observed by microscopy. Our study confirms that the camera gain or aperture of the ektacytometer should be used to standardize the size of the RBC diffraction pattern height when measuring RBC deformability in sickle cell patients and underscores the potential clinical utility of this technique. SN - 1875-8622 UR - https://www.unboundmedicine.com/medline/citation/26444610/Importance_of_methodological_standardization_for_the_ektacytometric_measures_of_red_blood_cell_deformability_in_sickle_cell_anemia_ L2 - https://content.iospress.com/openurl?genre=article&id=doi:10.3233/CH-151979 DB - PRIME DP - Unbound Medicine ER -