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Comparison of Cytotoxicity and the Anti-Adipogenic Effect of Green Tea Polyphenols with Epigallocatechin-3-Gallate in 3T3-L1 Preadipocytes.
Am J Chin Med. 2015; 43(6):1177-90.AJ

Abstract

Recent studies have demonstrated the effects of green tea polyphenols (GTP) and epigallocatechin-3-gallate (EGCG) on obesity. However, high doses of EGCG have also exhibited cytotoxicity. The aim of this study was to compare total GTP with purified EGCG on cytotoxicity, and to investigate the effects and the molecular mechanism of total GTP and EGCG on adipogenesis. Cytotoxicity was determined by cell viability assay. For the adipogenesis study, 3T3-L1 preadipocytes were incubated with three doses of GTP (1, 10, and 100 μg/ml) and the effect of EGCG (6.8 μg/ml) was compared with 10 μg/ml GTP containing 68% EGCG. Oil Red O staining and triglyceride content assay were carried out 10 days after differentiation and treatment. Adipogenic regulators CCAAT element binding protein α (C/EBPα), peroxisome proliferator-activated receptor gamma (PPARγ) and sterol regulatory element-binding protein-1c (SREBP-1c) were determined by qRT-PCR and immunoblotting. GTP at 1000 μg/ml and EGCG (68 and 680 μg/ml) significantly affected cell viability. Purified EGCG had greater cytotoxicity than corresponding doses of GTP. About 10 μg/ml of GTP showed stronger reduction in triglyceride accumulation than EGCG treatment. Transcriptional factors of C/EBPα, SREBP-1c and PPARγ were markedly decreased in both GTP and EGCG-treated cells and GTP exhibited stronger inhibitory effects on C/EBPα and PPARγ protein expression than EGCG (p < 0.05). In conclusion, total GTP exerted greater inhibitory effects than purified EGCG on adipogenesis through down-regulating the adipogenic factor C/EBPα, SREBP-1c and PPARγ expression. These findings support that a polyphenol mixture is safer and more effective than EGCG alone for preventing obesity and obesity-related chronic diseases.

Authors+Show Affiliations

School of Medical & Molecular Biosciences, University of Technology, Sydney, Australia.School of Medical & Molecular Biosciences, University of Technology, Sydney, Australia.School of Medical & Molecular Biosciences, University of Technology, Sydney, Australia.School of Medical & Molecular Biosciences, University of Technology, Sydney, Australia.School of Medical & Molecular Biosciences, University of Technology, Sydney, Australia.School of Medical & Molecular Biosciences, University of Technology, Sydney, Australia.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26446202

Citation

Lao, Weiguo, et al. "Comparison of Cytotoxicity and the Anti-Adipogenic Effect of Green Tea Polyphenols With Epigallocatechin-3-Gallate in 3T3-L1 Preadipocytes." The American Journal of Chinese Medicine, vol. 43, no. 6, 2015, pp. 1177-90.
Lao W, Tan Y, Jin X, et al. Comparison of Cytotoxicity and the Anti-Adipogenic Effect of Green Tea Polyphenols with Epigallocatechin-3-Gallate in 3T3-L1 Preadipocytes. Am J Chin Med. 2015;43(6):1177-90.
Lao, W., Tan, Y., Jin, X., Xiao, L., Kim, J. J., & Qu, X. (2015). Comparison of Cytotoxicity and the Anti-Adipogenic Effect of Green Tea Polyphenols with Epigallocatechin-3-Gallate in 3T3-L1 Preadipocytes. The American Journal of Chinese Medicine, 43(6), 1177-90. https://doi.org/10.1142/S0192415X15500676
Lao W, et al. Comparison of Cytotoxicity and the Anti-Adipogenic Effect of Green Tea Polyphenols With Epigallocatechin-3-Gallate in 3T3-L1 Preadipocytes. Am J Chin Med. 2015;43(6):1177-90. PubMed PMID: 26446202.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of Cytotoxicity and the Anti-Adipogenic Effect of Green Tea Polyphenols with Epigallocatechin-3-Gallate in 3T3-L1 Preadipocytes. AU - Lao,Weiguo, AU - Tan,Yi, AU - Jin,Xingliang, AU - Xiao,Linda, AU - Kim,Jane J Y, AU - Qu,Xianqin, PY - 2015/10/9/entrez PY - 2015/10/9/pubmed PY - 2016/4/9/medline KW - (–)-Epigallocatechin-3-Gallate KW - 3T3-L1 Preadipocytes, Cytotoxicity KW - Adipogenesis KW - Green Tea Polyphenols KW - Obesity Prevention SP - 1177 EP - 90 JF - The American journal of Chinese medicine JO - Am J Chin Med VL - 43 IS - 6 N2 - Recent studies have demonstrated the effects of green tea polyphenols (GTP) and epigallocatechin-3-gallate (EGCG) on obesity. However, high doses of EGCG have also exhibited cytotoxicity. The aim of this study was to compare total GTP with purified EGCG on cytotoxicity, and to investigate the effects and the molecular mechanism of total GTP and EGCG on adipogenesis. Cytotoxicity was determined by cell viability assay. For the adipogenesis study, 3T3-L1 preadipocytes were incubated with three doses of GTP (1, 10, and 100 μg/ml) and the effect of EGCG (6.8 μg/ml) was compared with 10 μg/ml GTP containing 68% EGCG. Oil Red O staining and triglyceride content assay were carried out 10 days after differentiation and treatment. Adipogenic regulators CCAAT element binding protein α (C/EBPα), peroxisome proliferator-activated receptor gamma (PPARγ) and sterol regulatory element-binding protein-1c (SREBP-1c) were determined by qRT-PCR and immunoblotting. GTP at 1000 μg/ml and EGCG (68 and 680 μg/ml) significantly affected cell viability. Purified EGCG had greater cytotoxicity than corresponding doses of GTP. About 10 μg/ml of GTP showed stronger reduction in triglyceride accumulation than EGCG treatment. Transcriptional factors of C/EBPα, SREBP-1c and PPARγ were markedly decreased in both GTP and EGCG-treated cells and GTP exhibited stronger inhibitory effects on C/EBPα and PPARγ protein expression than EGCG (p < 0.05). In conclusion, total GTP exerted greater inhibitory effects than purified EGCG on adipogenesis through down-regulating the adipogenic factor C/EBPα, SREBP-1c and PPARγ expression. These findings support that a polyphenol mixture is safer and more effective than EGCG alone for preventing obesity and obesity-related chronic diseases. SN - 1793-6853 UR - https://www.unboundmedicine.com/medline/citation/26446202/Comparison_of_Cytotoxicity_and_the_Anti_Adipogenic_Effect_of_Green_Tea_Polyphenols_with_Epigallocatechin_3_Gallate_in_3T3_L1_Preadipocytes_ L2 - https://www.worldscientific.com/doi/10.1142/S0192415X15500676?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -