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Analysis of Amphetamine-Derived Designer Drugs by Gas Chromatography with Mass Spectrometry.
J Anal Toxicol. 2016 Jan-Feb; 40(1):78-85.JA

Abstract

The 22 amphetamine-derived synthetic drugs (ADSDs), mostly cathinones, were examined by gas chromatography with mass spectrometry using two different derivatization methods with (i) heptafluorobutyric anhydride (HFBA) and (ii) pentafluorobenzoyl chloride (PFBCl). Both developed derivatization approaches were evaluated and compared for urine and serum samples. Extraction procedures proved to give satisfactory results with regard to recoveries and extract purity, even though both derivatization methods reached acceptable sensitivity for the intended use. The derivatization with PFBCl showed better results with respect to retention and response stability, thus the PFBCl method was selected for validation. Calibration curves were linear over the tested concentration range of 20-1,000 ng/mL with the R(2) values ranging from 0.994 to 0.998. Intra- and interday precisions and accuracies were within 20% for all concentrations in the linear range. The limit of detection was determined to be lower than 2 ng/mL for all 22 analytes. The method proved to be a useful analytical tool in the course of systematic toxicological analysis.

Authors+Show Affiliations

Faculty of Medicine and Dentistry, Department of Forensic Medicine and Medical Law, Palacký University, Olomouc CZ-77509, Czech Republic Faculty Hospital, Palacký University, Hnevotinska 3, Olomouc CZ-77509, Czech Republic.Faculty of Medicine and Dentistry, Department of Forensic Medicine and Medical Law, Palacký University, Olomouc CZ-77509, Czech Republic Faculty Hospital, Palacký University, Hnevotinska 3, Olomouc CZ-77509, Czech Republic.Regional Centre of Advanced Technologies and Materials, Faculty of Science, Department of Analytical Chemistry, Palacký University, 17 Listopadu 12, Olomouc CZ-77146, Czech Republic.Regional Centre of Advanced Technologies and Materials, Faculty of Science, Department of Analytical Chemistry, Palacký University, 17 Listopadu 12, Olomouc CZ-77146, Czech Republic.Regional Centre of Advanced Technologies and Materials, Faculty of Science, Department of Analytical Chemistry, Palacký University, 17 Listopadu 12, Olomouc CZ-77146, Czech Republic vitezslav.maier@upol.cz.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26446487

Citation

Ondra, Peter, et al. "Analysis of Amphetamine-Derived Designer Drugs By Gas Chromatography With Mass Spectrometry." Journal of Analytical Toxicology, vol. 40, no. 1, 2016, pp. 78-85.
Ondra P, Válka I, Knob R, et al. Analysis of Amphetamine-Derived Designer Drugs by Gas Chromatography with Mass Spectrometry. J Anal Toxicol. 2016;40(1):78-85.
Ondra, P., Válka, I., Knob, R., Ginterová, P., & Maier, V. (2016). Analysis of Amphetamine-Derived Designer Drugs by Gas Chromatography with Mass Spectrometry. Journal of Analytical Toxicology, 40(1), 78-85. https://doi.org/10.1093/jat/bkv113
Ondra P, et al. Analysis of Amphetamine-Derived Designer Drugs By Gas Chromatography With Mass Spectrometry. J Anal Toxicol. 2016 Jan-Feb;40(1):78-85. PubMed PMID: 26446487.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of Amphetamine-Derived Designer Drugs by Gas Chromatography with Mass Spectrometry. AU - Ondra,Peter, AU - Válka,Ivo, AU - Knob,Radim, AU - Ginterová,Pavlína, AU - Maier,Vítězslav, Y1 - 2015/10/06/ PY - 2015/10/9/entrez PY - 2015/10/9/pubmed PY - 2016/8/27/medline SP - 78 EP - 85 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 40 IS - 1 N2 - The 22 amphetamine-derived synthetic drugs (ADSDs), mostly cathinones, were examined by gas chromatography with mass spectrometry using two different derivatization methods with (i) heptafluorobutyric anhydride (HFBA) and (ii) pentafluorobenzoyl chloride (PFBCl). Both developed derivatization approaches were evaluated and compared for urine and serum samples. Extraction procedures proved to give satisfactory results with regard to recoveries and extract purity, even though both derivatization methods reached acceptable sensitivity for the intended use. The derivatization with PFBCl showed better results with respect to retention and response stability, thus the PFBCl method was selected for validation. Calibration curves were linear over the tested concentration range of 20-1,000 ng/mL with the R(2) values ranging from 0.994 to 0.998. Intra- and interday precisions and accuracies were within 20% for all concentrations in the linear range. The limit of detection was determined to be lower than 2 ng/mL for all 22 analytes. The method proved to be a useful analytical tool in the course of systematic toxicological analysis. SN - 1945-2403 UR - https://www.unboundmedicine.com/medline/citation/26446487/Analysis_of_Amphetamine_Derived_Designer_Drugs_by_Gas_Chromatography_with_Mass_Spectrometry_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/bkv113 DB - PRIME DP - Unbound Medicine ER -