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Visual detection of thrombin using a strip biosensor through aptamer-cleavage reaction with enzyme catalytic amplification.
Analyst. 2015 Nov 21; 140(22):7710-7.A

Abstract

A new class of strip biosensors has been established based on well-distributed thrombin aptamer-linked gold nanoparticle aggregates, which will undergo a cracking reaction when the target recognizes its homologous aptamer. Combining the aptamer-cleavage reaction with the enzyme catalytic amplification system, our proposed lateral flow strip biosensor (LFB) is capable of visually detecting 6.4 pM of thrombin without instrumentation within 12 minutes. Under the optimal conditions, the quantitative detection of thrombin by a portable strip reader exhibited a linear relationship between the peak area and the concentration of thrombin in the range of 6.4 pM-500 nM with a detection limit of 4.9 pM, which is three orders of magnitude lower than that of the aptamer-functionalized gold nanoparticle-based LFB (2.5 nM, Xu et al., Anal. Chem., 2009, 81, 669-675). As the aptamers have no special requirements and the gold nanoparticles can also be replaced by other metallic nanoparticles, this method for strip sensing is expected to be generally applicable in point of care testing, home testing, medical diagnostics, clinical diagnosis, and environmental monitoring.

Authors+Show Affiliations

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR China. wangsf@hubu.edu.cn.Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR China. wangsf@hubu.edu.cn.Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR China. wangsf@hubu.edu.cn.Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR China. wangsf@hubu.edu.cn.Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR China. wangsf@hubu.edu.cn.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26451394

Citation

Qin, Chunyan, et al. "Visual Detection of Thrombin Using a Strip Biosensor Through Aptamer-cleavage Reaction With Enzyme Catalytic Amplification." The Analyst, vol. 140, no. 22, 2015, pp. 7710-7.
Qin C, Wen W, Zhang X, et al. Visual detection of thrombin using a strip biosensor through aptamer-cleavage reaction with enzyme catalytic amplification. Analyst. 2015;140(22):7710-7.
Qin, C., Wen, W., Zhang, X., Gu, H., & Wang, S. (2015). Visual detection of thrombin using a strip biosensor through aptamer-cleavage reaction with enzyme catalytic amplification. The Analyst, 140(22), 7710-7. https://doi.org/10.1039/c5an01712b
Qin C, et al. Visual Detection of Thrombin Using a Strip Biosensor Through Aptamer-cleavage Reaction With Enzyme Catalytic Amplification. Analyst. 2015 Nov 21;140(22):7710-7. PubMed PMID: 26451394.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Visual detection of thrombin using a strip biosensor through aptamer-cleavage reaction with enzyme catalytic amplification. AU - Qin,Chunyan, AU - Wen,Wei, AU - Zhang,Xiuhua, AU - Gu,Haoshuang, AU - Wang,Shengfu, PY - 2015/10/10/entrez PY - 2015/10/10/pubmed PY - 2016/7/23/medline SP - 7710 EP - 7 JF - The Analyst JO - Analyst VL - 140 IS - 22 N2 - A new class of strip biosensors has been established based on well-distributed thrombin aptamer-linked gold nanoparticle aggregates, which will undergo a cracking reaction when the target recognizes its homologous aptamer. Combining the aptamer-cleavage reaction with the enzyme catalytic amplification system, our proposed lateral flow strip biosensor (LFB) is capable of visually detecting 6.4 pM of thrombin without instrumentation within 12 minutes. Under the optimal conditions, the quantitative detection of thrombin by a portable strip reader exhibited a linear relationship between the peak area and the concentration of thrombin in the range of 6.4 pM-500 nM with a detection limit of 4.9 pM, which is three orders of magnitude lower than that of the aptamer-functionalized gold nanoparticle-based LFB (2.5 nM, Xu et al., Anal. Chem., 2009, 81, 669-675). As the aptamers have no special requirements and the gold nanoparticles can also be replaced by other metallic nanoparticles, this method for strip sensing is expected to be generally applicable in point of care testing, home testing, medical diagnostics, clinical diagnosis, and environmental monitoring. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/26451394/Visual_detection_of_thrombin_using_a_strip_biosensor_through_aptamer_cleavage_reaction_with_enzyme_catalytic_amplification_ L2 - https://doi.org/10.1039/c5an01712b DB - PRIME DP - Unbound Medicine ER -