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Protective Effect of Astragaloside IV Against Paraquat-Induced Lung Injury in Mice by Suppressing Rho Signaling.
Inflammation. 2016 Feb; 39(1):483-492.I

Abstract

The purpose of the present study was to evaluate the protective effects of astragaloside IV (AS IV) against paraquat (PQ)-induced pulmonary injury in vivo. Fifty BALB/C mice were randomized into five groups: (1) control, (2) PQ, (3) PQ + dexamethasone (Dex, 5 mg/kg), (4) PQ + AS IV (50 mg/kg), and (5) PQ + AS IV (100 mg/kg). A single dose of PQ (50 mg/kg, i.p.) was intraperitoneally given to induced acute lung injury. Then, mice were treated with AS IV (50 and 100 mg/kg/day, orally) for 5 days. At the end of the experiment, animals were euthanized; then, the bronchoalveolar lavage fluid (BALF) and lung tissues were collected for histological observation, biochemical assay, and Western blot analysis. Malondialdehyde (MDA), myeloperoxidase (MPO), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) in lung tissues, and interleukin-6 (IL-6), IL-1β, tumor necrosis factor-α (TNF-α) levels in BALF were determined. Histological examination indicated that AS IV attenuated lung damage caused by PQ. Biochemical results showed that AS IV treatment significantly reduced the levels of MDA, MPO, and inflammatory cytokines while increased the levels of SOD, CAT, and GSH-Px compared with those in PQ group. Western blot results revealed that AS IV attenuated the Txnip/Trx expressions and inhibited Rho/ROCK/nuclear factor kappaB (NF-κB) signaling pathway in PQ-challenged mice. These findings suggested the protective effect of AS IV as a natural product on PQ-induced pulmonary injury.

Authors+Show Affiliations

State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China.State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China.State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China.School of Pharmacy, China Pharmaceutical University, Nanjing, 210009, China.State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China.State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China.State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China.State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China.State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China.School of Pharmacy, China Pharmaceutical University, Nanjing, 210009, China.State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China. huijicpu@163.com.State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China. mazhanqiangnanjing@126.com. Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing, 210009, China. mazhanqiangnanjing@126.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26452991

Citation

Chen, Tong, et al. "Protective Effect of Astragaloside IV Against Paraquat-Induced Lung Injury in Mice By Suppressing Rho Signaling." Inflammation, vol. 39, no. 1, 2016, pp. 483-492.
Chen T, Wang R, Jiang W, et al. Protective Effect of Astragaloside IV Against Paraquat-Induced Lung Injury in Mice by Suppressing Rho Signaling. Inflammation. 2016;39(1):483-492.
Chen, T., Wang, R., Jiang, W., Wang, H., Xu, A., Lu, G., Ren, Y., Xu, Y., Song, Y., Yong, S., Ji, H., & Ma, Z. (2016). Protective Effect of Astragaloside IV Against Paraquat-Induced Lung Injury in Mice by Suppressing Rho Signaling. Inflammation, 39(1), 483-492. https://doi.org/10.1007/s10753-015-0272-4
Chen T, et al. Protective Effect of Astragaloside IV Against Paraquat-Induced Lung Injury in Mice By Suppressing Rho Signaling. Inflammation. 2016;39(1):483-492. PubMed PMID: 26452991.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective Effect of Astragaloside IV Against Paraquat-Induced Lung Injury in Mice by Suppressing Rho Signaling. AU - Chen,Tong, AU - Wang,Ruoning, AU - Jiang,Wenjiao, AU - Wang,Huimin, AU - Xu,Ang, AU - Lu,Guo, AU - Ren,Yi, AU - Xu,Yangmei, AU - Song,Yangyang, AU - Yong,Shoulei, AU - Ji,Hui, AU - Ma,Zhanqiang, PY - 2015/10/11/entrez PY - 2015/10/11/pubmed PY - 2016/12/15/medline KW - Rho KW - Txnip/Trx KW - astragaloside IV KW - lung injury KW - paraquat SP - 483 EP - 492 JF - Inflammation JO - Inflammation VL - 39 IS - 1 N2 - The purpose of the present study was to evaluate the protective effects of astragaloside IV (AS IV) against paraquat (PQ)-induced pulmonary injury in vivo. Fifty BALB/C mice were randomized into five groups: (1) control, (2) PQ, (3) PQ + dexamethasone (Dex, 5 mg/kg), (4) PQ + AS IV (50 mg/kg), and (5) PQ + AS IV (100 mg/kg). A single dose of PQ (50 mg/kg, i.p.) was intraperitoneally given to induced acute lung injury. Then, mice were treated with AS IV (50 and 100 mg/kg/day, orally) for 5 days. At the end of the experiment, animals were euthanized; then, the bronchoalveolar lavage fluid (BALF) and lung tissues were collected for histological observation, biochemical assay, and Western blot analysis. Malondialdehyde (MDA), myeloperoxidase (MPO), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) in lung tissues, and interleukin-6 (IL-6), IL-1β, tumor necrosis factor-α (TNF-α) levels in BALF were determined. Histological examination indicated that AS IV attenuated lung damage caused by PQ. Biochemical results showed that AS IV treatment significantly reduced the levels of MDA, MPO, and inflammatory cytokines while increased the levels of SOD, CAT, and GSH-Px compared with those in PQ group. Western blot results revealed that AS IV attenuated the Txnip/Trx expressions and inhibited Rho/ROCK/nuclear factor kappaB (NF-κB) signaling pathway in PQ-challenged mice. These findings suggested the protective effect of AS IV as a natural product on PQ-induced pulmonary injury. SN - 1573-2576 UR - https://www.unboundmedicine.com/medline/citation/26452991/Protective_Effect_of_Astragaloside_IV_Against_Paraquat_Induced_Lung_Injury_in_Mice_by_Suppressing_Rho_Signaling_ L2 - https://doi.org/10.1007/s10753-015-0272-4 DB - PRIME DP - Unbound Medicine ER -