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Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L.
Chemosphere. 2016 Feb; 144:1083-90.C

Abstract

Maize plants expressing dsRNA for the management of Diabrotica virgifera virgifera are likely to be commercially available by the end of this decade. Honey bees, Apis mellifera, can potentially be exposed to pollen from transformed maize expressing dsRNA. Consequently, evaluation of the biological impacts of RNAi in honey bees is a fundamental component for ecological risk assessment. The insecticidal activity of a known lethal dsRNA target for D. v. virgifera, the vATPase subunit A, was evaluated in larval and adult honey bees. Activity of both D. v. virgifera (Dvv)- and A. mellifera (Am)-specific dsRNA was tested by dietary exposure to dsRNA. Larval development, survival, adult eclosion, adult life span and relative gene expression were evaluated. The results of these tests indicated that Dvv vATPase-A dsRNA has limited effects on larval and adult honey bee survival. Importantly, no effects were observed upon exposure of Am vATPase-A dsRNA suggesting that the lack of response involves factors other than sequence specificity. The results from this study provide guidance for future RNAi risk analyses and for the development of a risk assessment framework that incorporates similar hazard assessments.

Authors+Show Affiliations

University of Nebraska, Department of Entomology, 103 Entomology Hall, Lincoln, NE 68583-0816, United States. Electronic address: anamaria.velez@gmail.com.University of Nebraska, Department of Entomology, 103 Entomology Hall, Lincoln, NE 68583-0816, United States.University of Nebraska, Department of Entomology, 103 Entomology Hall, Lincoln, NE 68583-0816, United States.University of Kentucky, Department of Entomology, S-225 Agricultural Science Center N, Lexington, KY 40546-0091, United States.University of Nebraska, Department of Entomology, 103 Entomology Hall, Lincoln, NE 68583-0816, United States.University of Nebraska, Department of Entomology, 103 Entomology Hall, Lincoln, NE 68583-0816, United States.University of Nebraska, Department of Entomology, 103 Entomology Hall, Lincoln, NE 68583-0816, United States.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

26454117

Citation

Vélez, Ana María, et al. "Developing an in Vivo Toxicity Assay for RNAi Risk Assessment in Honey Bees, Apis Mellifera L." Chemosphere, vol. 144, 2016, pp. 1083-90.
Vélez AM, Jurzenski J, Matz N, et al. Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L. Chemosphere. 2016;144:1083-90.
Vélez, A. M., Jurzenski, J., Matz, N., Zhou, X., Wang, H., Ellis, M., & Siegfried, B. D. (2016). Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L. Chemosphere, 144, 1083-90. https://doi.org/10.1016/j.chemosphere.2015.09.068
Vélez AM, et al. Developing an in Vivo Toxicity Assay for RNAi Risk Assessment in Honey Bees, Apis Mellifera L. Chemosphere. 2016;144:1083-90. PubMed PMID: 26454117.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L. AU - Vélez,Ana María, AU - Jurzenski,Jessica, AU - Matz,Natalie, AU - Zhou,Xuguo, AU - Wang,Haichuan, AU - Ellis,Marion, AU - Siegfried,Blair D, Y1 - 2015/10/23/ PY - 2015/06/10/received PY - 2015/08/23/revised PY - 2015/09/18/accepted PY - 2015/10/11/entrez PY - 2015/10/11/pubmed PY - 2016/9/20/medline KW - Diabrotica virgifera virgifera KW - Non-target arthropod KW - RNAi risk assessment KW - dsRNA KW - vATPase-A SP - 1083 EP - 90 JF - Chemosphere JO - Chemosphere VL - 144 N2 - Maize plants expressing dsRNA for the management of Diabrotica virgifera virgifera are likely to be commercially available by the end of this decade. Honey bees, Apis mellifera, can potentially be exposed to pollen from transformed maize expressing dsRNA. Consequently, evaluation of the biological impacts of RNAi in honey bees is a fundamental component for ecological risk assessment. The insecticidal activity of a known lethal dsRNA target for D. v. virgifera, the vATPase subunit A, was evaluated in larval and adult honey bees. Activity of both D. v. virgifera (Dvv)- and A. mellifera (Am)-specific dsRNA was tested by dietary exposure to dsRNA. Larval development, survival, adult eclosion, adult life span and relative gene expression were evaluated. The results of these tests indicated that Dvv vATPase-A dsRNA has limited effects on larval and adult honey bee survival. Importantly, no effects were observed upon exposure of Am vATPase-A dsRNA suggesting that the lack of response involves factors other than sequence specificity. The results from this study provide guidance for future RNAi risk analyses and for the development of a risk assessment framework that incorporates similar hazard assessments. SN - 1879-1298 UR - https://www.unboundmedicine.com/medline/citation/26454117/Developing_an_in_vivo_toxicity_assay_for_RNAi_risk_assessment_in_honey_bees_Apis_mellifera_L_ DB - PRIME DP - Unbound Medicine ER -