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A simple method for the determination of Bosutinib in rat plasma by UPLC-MS/MS.

Abstract

In this work, a simple, sensitive and fast ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the quantitative determination of bosutinib in rat plasma. Plasma samples were processed with a protein precipitation. The separation was achieved by an Acquity UPLC BEH C18 column (2.1mm×50mm, 1.7μm) column with a gradient mobile phase consisting of 0.1% formic acid in water and acetonitrile. Detection was carried out using positive-ion electrospray tandem mass spectrometry via multiple reaction monitoring (MRM). The validated method had an excellent linearity in the range of 0.1-500ng/mL (R(2)>0.9977) with a lower limit of quantification (0.1ng/mL). The extraction recovery was in the range of 75.6-85.6% for bosutinib and 81.2% for pirfenidone (internal standard, IS). The intra- and inter-day precision was below 9.7% and accuracy was from -8.1% to 8.8%. No notable matrix effect and astaticism was observed for bosutinib. The method has been successfully applied to a pharmacokinetic study of bosutinib in rats for the first time, which provides the basis for the further development and application of bosutinib.

Authors+Show Affiliations

The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. Electronic address: zmt0417@hotmail.com.

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

26454795

Citation

Xu, Yihua, et al. "A Simple Method for the Determination of Bosutinib in Rat Plasma By UPLC-MS/MS." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 1004, 2015, pp. 93-7.
Xu Y, Huang XC, Dai S, et al. A simple method for the determination of Bosutinib in rat plasma by UPLC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2015;1004:93-7.
Xu, Y., Huang, X. C., Dai, S., Xiao, Y., & Zhou, M. T. (2015). A simple method for the determination of Bosutinib in rat plasma by UPLC-MS/MS. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 1004, pp. 93-7. doi:10.1016/j.jchromb.2015.09.030.
Xu Y, et al. A Simple Method for the Determination of Bosutinib in Rat Plasma By UPLC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Nov 1;1004:93-7. PubMed PMID: 26454795.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A simple method for the determination of Bosutinib in rat plasma by UPLC-MS/MS. AU - Xu,Yihua, AU - Huang,Xin-ce, AU - Dai,Shengjie, AU - Xiao,Yuwu, AU - Zhou,Meng-tao, Y1 - 2015/09/25/ PY - 2015/05/24/received PY - 2015/09/20/accepted PY - 2015/10/12/entrez PY - 2015/10/12/pubmed PY - 2016/5/14/medline KW - Bosutinib KW - Pharmacokinetics KW - Plasma KW - Rat KW - UPLC-MS/MS SP - 93 EP - 7 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 1004 N2 - In this work, a simple, sensitive and fast ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the quantitative determination of bosutinib in rat plasma. Plasma samples were processed with a protein precipitation. The separation was achieved by an Acquity UPLC BEH C18 column (2.1mm×50mm, 1.7μm) column with a gradient mobile phase consisting of 0.1% formic acid in water and acetonitrile. Detection was carried out using positive-ion electrospray tandem mass spectrometry via multiple reaction monitoring (MRM). The validated method had an excellent linearity in the range of 0.1-500ng/mL (R(2)>0.9977) with a lower limit of quantification (0.1ng/mL). The extraction recovery was in the range of 75.6-85.6% for bosutinib and 81.2% for pirfenidone (internal standard, IS). The intra- and inter-day precision was below 9.7% and accuracy was from -8.1% to 8.8%. No notable matrix effect and astaticism was observed for bosutinib. The method has been successfully applied to a pharmacokinetic study of bosutinib in rats for the first time, which provides the basis for the further development and application of bosutinib. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/26454795/A_simple_method_for_the_determination_of_Bosutinib_in_rat_plasma_by_UPLC_MS/MS_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(15)30206-3 DB - PRIME DP - Unbound Medicine ER -