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Ethyl acetate fraction of Aframomum melegueta fruit ameliorates pancreatic β-cell dysfunction and major diabetes-related parameters in a type 2 diabetes model of rats.
J Ethnopharmacol. 2015 Dec 04; 175:518-27.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

In West Africa, various preparations of the fruit, seed and leaf of Aframomum melegueta K. Schum. are reputably used for the management of diabetes mellitus (DM) and other metabolic disorders. The present study evaluated the anti-diabetic effects of A. melegueta ethyl acetate fraction (AMEF) from fruit ethanolic extract in a type 2 diabetes (T2D) model of rats.

MATERIALS AND METHODS

T2D was induced in rats by feeding a 10% fructose solution ad libitum for two weeks followed by a single intraperitoneal injection of streptozotocin (40 mg/kg body weight) and the animals were orally treated with 150 or 300 mg/kg body weight (bw) of the AMEF once daily for four weeks.

RESULTS

At the end of the intervention, diabetic untreated animals showed significantly higher serum glucose, serum fructosamine, LDH, CK-MB, serum lipids, liver glycogen, insulin resistance (HOMA-IR), AI, CRI and lower serum insulin, pancreatic β-cell function (HOMA- β) and glucose tolerance ability compared to the normal animals. Histopathological examination of their pancreas revealed corresponding pathological changes in the islets and β-cells. These alterations were reverted to near-normal after the treatment of AMEF at 150 and 300 mg/kg bw when, the effects were more pronounced at 300 mg/kg bw compared to the 150 mg/kg bw.

CONCLUSION

The results of our study suggest that AMEF treatment at 300 mg/kg bw showed potent anti-diabetic effect in a T2D model of rats.

Authors+Show Affiliations

Department of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, (Westville Campus), Durban 4000, South Africa; Department of Biochemistry, Faculty of Science, Ahmadu Bello University, Zaria, Nigeria.Department of Chemistry, School of Chemistry and Physics, University of KwaZulu-Natal, (Westville Campus), Durban 4000, South Africa.Department of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, (Westville Campus), Durban 4000, South Africa. Electronic address: islamd@ukzn.ac.za.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26456345

Citation

Mohammed, Aminu, et al. "Ethyl Acetate Fraction of Aframomum Melegueta Fruit Ameliorates Pancreatic Β-cell Dysfunction and Major Diabetes-related Parameters in a Type 2 Diabetes Model of Rats." Journal of Ethnopharmacology, vol. 175, 2015, pp. 518-27.
Mohammed A, Koorbanally NA, Islam MS. Ethyl acetate fraction of Aframomum melegueta fruit ameliorates pancreatic β-cell dysfunction and major diabetes-related parameters in a type 2 diabetes model of rats. J Ethnopharmacol. 2015;175:518-27.
Mohammed, A., Koorbanally, N. A., & Islam, M. S. (2015). Ethyl acetate fraction of Aframomum melegueta fruit ameliorates pancreatic β-cell dysfunction and major diabetes-related parameters in a type 2 diabetes model of rats. Journal of Ethnopharmacology, 175, 518-27. https://doi.org/10.1016/j.jep.2015.10.011
Mohammed A, Koorbanally NA, Islam MS. Ethyl Acetate Fraction of Aframomum Melegueta Fruit Ameliorates Pancreatic Β-cell Dysfunction and Major Diabetes-related Parameters in a Type 2 Diabetes Model of Rats. J Ethnopharmacol. 2015 Dec 4;175:518-27. PubMed PMID: 26456345.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ethyl acetate fraction of Aframomum melegueta fruit ameliorates pancreatic β-cell dysfunction and major diabetes-related parameters in a type 2 diabetes model of rats. AU - Mohammed,Aminu, AU - Koorbanally,Neil Anthony, AU - Islam,Md Shahidul, Y1 - 2015/10/08/ PY - 2015/04/12/received PY - 2015/09/27/revised PY - 2015/10/04/accepted PY - 2015/10/13/entrez PY - 2015/10/13/pubmed PY - 2016/10/7/medline KW - Absolute ethanol (CID 702) KW - Aframomum melegueta KW - Citric acid (CID 311) KW - Dichoromethane (CID 6344) KW - Dinitrosalicylic acid (DNS) (CID 11873) KW - Ethyl acetate (CID 8857) KW - Ethyl acetate fraction KW - Fruit ethnolic extract KW - Hexane (CID 8058) KW - Metformin (CID 4091) KW - Rats KW - Sodium citrate (CID 6224) KW - Streptozotocin (CID 29327) KW - Type 2 diabetes KW - p-nitrophenyl-α-d-glucopyranoside (pNPG) (CID 1197369) SP - 518 EP - 27 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 175 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: In West Africa, various preparations of the fruit, seed and leaf of Aframomum melegueta K. Schum. are reputably used for the management of diabetes mellitus (DM) and other metabolic disorders. The present study evaluated the anti-diabetic effects of A. melegueta ethyl acetate fraction (AMEF) from fruit ethanolic extract in a type 2 diabetes (T2D) model of rats. MATERIALS AND METHODS: T2D was induced in rats by feeding a 10% fructose solution ad libitum for two weeks followed by a single intraperitoneal injection of streptozotocin (40 mg/kg body weight) and the animals were orally treated with 150 or 300 mg/kg body weight (bw) of the AMEF once daily for four weeks. RESULTS: At the end of the intervention, diabetic untreated animals showed significantly higher serum glucose, serum fructosamine, LDH, CK-MB, serum lipids, liver glycogen, insulin resistance (HOMA-IR), AI, CRI and lower serum insulin, pancreatic β-cell function (HOMA- β) and glucose tolerance ability compared to the normal animals. Histopathological examination of their pancreas revealed corresponding pathological changes in the islets and β-cells. These alterations were reverted to near-normal after the treatment of AMEF at 150 and 300 mg/kg bw when, the effects were more pronounced at 300 mg/kg bw compared to the 150 mg/kg bw. CONCLUSION: The results of our study suggest that AMEF treatment at 300 mg/kg bw showed potent anti-diabetic effect in a T2D model of rats. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/26456345/Ethyl_acetate_fraction_of_Aframomum_melegueta_fruit_ameliorates_pancreatic_β_cell_dysfunction_and_major_diabetes_related_parameters_in_a_type_2_diabetes_model_of_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(15)30170-7 DB - PRIME DP - Unbound Medicine ER -