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Development and Molecular Cytogenetic Identification of a Novel Wheat-Leymus mollis Lm#7Ns (7D) Disomic Substitution Line with Stripe Rust Resistance.
PLoS One. 2015; 10(10):e0140227.Plos

Abstract

Leymus mollis (2n = 4x = 28, NsNsXmXm) possesses novel and important genes for resistance against multi-fungal diseases. The development of new wheat-L. mollis introgression lines is of great significance for wheat disease resistance breeding. M11003-3-1-15-8, a novel disomic substitution line of common wheat cv. 7182 -L. mollis, developed and selected from the BC1F5 progeny between wheat cv. 7182 and octoploid Tritileymus M47 (2n = 8x = 56, AABBDDNsNs), was characterized by morphological and cytogenetic identification, analysis of functional molecular markers, genomic in situ hybridization (GISH), sequential fluorescence in situ hybridization (FISH)-genomic in situ hybridization (GISH) and disease resistance evaluation. Cytological observations suggested that M11003-3-1-15-8 contained 42 chromosomes and formed 21 bivalents at meiotic metaphase I. The GISH investigations showed that line contained 40 wheat chromosomes and a pair of L. mollis chromosomes. EST-STS multiple loci markers and PLUG (PCR-based Landmark Unique Gene) markers confirmed that the introduced L. mollis chromosomes belonged to homoeologous group 7, it was designated as Lm#7Ns. While nulli-tetrasomic and sequential FISH-GISH analysis using the oligonucleotide Oligo-pSc119.2 and Oligo-pTa535 as probes revealed that the wheat 7D chromosomes were absent in M11003-3-1-15-8. Therefore, it was deduced that M11003-3-1-15-8 was a wheat-L. mollis Lm#7Ns (7D) disomic substitution line. Field disease resistance demonstrated that the introduced L. mollis chromosomes Lm#7Ns were responsible for the stripe rust resistance at the adult stage. Moreover, M11003-3-1-15-8 had a superior numbers of florets. The novel disomic substitution line M11003-3-1-15-8, could be exploited as an important genetic material in wheat resistance breeding programs and genetic resources.

Links

Authors+Show Affiliations

College of Agronomy, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, Shaanxi, 712100, China.

College of Agronomy, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, Shaanxi, 712100, China.

College of Agronomy, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, Shaanxi, 712100, China.

College of Agronomy, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, Shaanxi, 712100, China.

College of Agronomy, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, Shaanxi, 712100, China.

College of Agronomy, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, Shaanxi, 712100, China.

College of Agronomy, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, Shaanxi, 712100, China.

MeSH

BasidiomycotaChromosomes, PlantDisease ResistanceGenetic MarkersHybridization, GeneticIn Situ Hybridization, FluorescencePlant DiseasesPlants, Genetically ModifiedPoaceaeTriticum

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26465140

Citation

Yang, Xiaofei, et al. "Development and Molecular Cytogenetic Identification of a Novel Wheat-Leymus Mollis Lm#7Ns (7D) Disomic Substitution Line With Stripe Rust Resistance." PloS One, vol. 10, no. 10, 2015, pp. e0140227.

Yang X, Wang C, Li X, et al. Development and Molecular Cytogenetic Identification of a Novel Wheat-Leymus mollis Lm#7Ns (7D) Disomic Substitution Line with Stripe Rust Resistance. PLoS One. 2015;10(10):e0140227.

Yang, X., Wang, C., Li, X., Chen, C., Tian, Z., Wang, Y., & Ji, W. (2015). Development and Molecular Cytogenetic Identification of a Novel Wheat-Leymus mollis Lm#7Ns (7D) Disomic Substitution Line with Stripe Rust Resistance. PloS One, 10(10), e0140227. https://doi.org/10.1371/journal.pone.0140227

Yang X, et al. Development and Molecular Cytogenetic Identification of a Novel Wheat-Leymus Mollis Lm#7Ns (7D) Disomic Substitution Line With Stripe Rust Resistance. PLoS One. 2015;10(10):e0140227. PubMed PMID: 26465140.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Development and Molecular Cytogenetic Identification of a Novel Wheat-Leymus mollis Lm#7Ns (7D) Disomic Substitution Line with Stripe Rust Resistance. AU - Yang,Xiaofei, AU - Wang,Changyou, AU - Li,Xin, AU - Chen,Chunhuan, AU - Tian,Zengrong, AU - Wang,Yajuan, AU - Ji,Wanquan, Y1 - 2015/10/14/ PY - 2015/07/06/received PY - 2015/09/23/accepted PY - 2015/10/15/entrez PY - 2015/10/16/pubmed PY - 2016/6/1/medline SP - e0140227 EP - e0140227 JF - PloS one JO - PLoS One VL - 10 IS - 10 N2 - Leymus mollis (2n = 4x = 28, NsNsXmXm) possesses novel and important genes for resistance against multi-fungal diseases. The development of new wheat-L. mollis introgression lines is of great significance for wheat disease resistance breeding. M11003-3-1-15-8, a novel disomic substitution line of common wheat cv. 7182 -L. mollis, developed and selected from the BC1F5 progeny between wheat cv. 7182 and octoploid Tritileymus M47 (2n = 8x = 56, AABBDDNsNs), was characterized by morphological and cytogenetic identification, analysis of functional molecular markers, genomic in situ hybridization (GISH), sequential fluorescence in situ hybridization (FISH)-genomic in situ hybridization (GISH) and disease resistance evaluation. Cytological observations suggested that M11003-3-1-15-8 contained 42 chromosomes and formed 21 bivalents at meiotic metaphase I. The GISH investigations showed that line contained 40 wheat chromosomes and a pair of L. mollis chromosomes. EST-STS multiple loci markers and PLUG (PCR-based Landmark Unique Gene) markers confirmed that the introduced L. mollis chromosomes belonged to homoeologous group 7, it was designated as Lm#7Ns. While nulli-tetrasomic and sequential FISH-GISH analysis using the oligonucleotide Oligo-pSc119.2 and Oligo-pTa535 as probes revealed that the wheat 7D chromosomes were absent in M11003-3-1-15-8. Therefore, it was deduced that M11003-3-1-15-8 was a wheat-L. mollis Lm#7Ns (7D) disomic substitution line. Field disease resistance demonstrated that the introduced L. mollis chromosomes Lm#7Ns were responsible for the stripe rust resistance at the adult stage. Moreover, M11003-3-1-15-8 had a superior numbers of florets. The novel disomic substitution line M11003-3-1-15-8, could be exploited as an important genetic material in wheat resistance breeding programs and genetic resources. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/26465140/Development_and_Molecular_Cytogenetic_Identification_of_a_Novel_Wheat_Leymus_mollis_Lm DB - PRIME DP - Unbound Medicine ER -