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Urinary neutrophil gelatinase-associated lipocalin concentration changes after acute haemorrhage and colloid-mediated reperfusion in anaesthetized dogs.
Vet Anaesth Analg. 2016 May; 43(3):262-70.VA

Abstract

OBJECTIVE

To determine changes in urine neutrophil gelatinase-associated lipocalin concentration (uNGAL) in anaesthetized Greyhound dogs that developed acute tubular damage following haemorrhage and resuscitation with colloid-based fluids.

STUDY DESIGN

Prospective experimental study.

ANIMALS

Seven healthy adult entire male Greyhound dogs.

METHODS

During isoflurane anaesthesia, approximately 50 mL kg(-1) of blood was removed to maintain mean arterial pressure (MAP) ≤40 mmHg for 1 hour followed by gelatin-based colloid administration to maintain MAP ≥60 mmHg for 3 hours. Data, including oxygen extraction ratio and uNGAL, were collected before (T0) and immediately following (T1) haemorrhage, and hourly during reperfusion (T2-T4). After T4, dogs were euthanized and renal tissue was collected for histology. Statistical analysis was performed using repeated-measures one-way anova. Data are presented as means (95% confidence interval).

RESULTS

Histology identified renal tubular epithelial damage in all dogs. Urine NGAL concentration increased from 12.1 (0-30.6) ng mL(-1) at T0 to 122.0 (64.1-180.0) ng mL(-1) by T3. Compared with T0, uNGAL was significantly higher at T3 (p = 0.016) and was increased 24-fold.

CONCLUSIONS AND CLINICAL RELEVANCE

Despite wide individual variation in baseline uNGAL, increases in uNGAL were observed in all dogs, suggesting that this biomarker has the potential to detect renal tubular injury following haemorrhage-induced hypotension and colloid-mediated reperfusion.

Authors+Show Affiliations

School of Veterinary & Life Sciences, Murdoch University, Perth, WA, Australia.School of Veterinary & Life Sciences, Murdoch University, Perth, WA, Australia.Department of Veterinary Biosciences, The Ohio State University, Columbus, OH, USA.School of Veterinary & Life Sciences, Murdoch University, Perth, WA, Australia.School of Veterinary & Life Sciences, Murdoch University, Perth, WA, Australia.Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, USA.School of Veterinary & Life Sciences, Murdoch University, Perth, WA, Australia.

Pub Type(s)

Clinical Trial
Journal Article

Language

eng

PubMed ID

26469528

Citation

Davis, Jennifer, et al. "Urinary Neutrophil Gelatinase-associated Lipocalin Concentration Changes After Acute Haemorrhage and Colloid-mediated Reperfusion in Anaesthetized Dogs." Veterinary Anaesthesia and Analgesia, vol. 43, no. 3, 2016, pp. 262-70.
Davis J, Raisis AL, Cianciolo RE, et al. Urinary neutrophil gelatinase-associated lipocalin concentration changes after acute haemorrhage and colloid-mediated reperfusion in anaesthetized dogs. Vet Anaesth Analg. 2016;43(3):262-70.
Davis, J., Raisis, A. L., Cianciolo, R. E., Miller, D. W., Shiel, R. E., Nabity, M. B., & Hosgood, G. L. (2016). Urinary neutrophil gelatinase-associated lipocalin concentration changes after acute haemorrhage and colloid-mediated reperfusion in anaesthetized dogs. Veterinary Anaesthesia and Analgesia, 43(3), 262-70. https://doi.org/10.1111/vaa.12311
Davis J, et al. Urinary Neutrophil Gelatinase-associated Lipocalin Concentration Changes After Acute Haemorrhage and Colloid-mediated Reperfusion in Anaesthetized Dogs. Vet Anaesth Analg. 2016;43(3):262-70. PubMed PMID: 26469528.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Urinary neutrophil gelatinase-associated lipocalin concentration changes after acute haemorrhage and colloid-mediated reperfusion in anaesthetized dogs. AU - Davis,Jennifer, AU - Raisis,Anthea L, AU - Cianciolo,Rachel E, AU - Miller,David W, AU - Shiel,Robert E, AU - Nabity,Mary B, AU - Hosgood,Giselle L, Y1 - 2015/10/15/ PY - 2015/03/15/received PY - 2015/06/27/accepted PY - 2015/10/16/entrez PY - 2015/10/16/pubmed PY - 2016/12/29/medline KW - NGAL KW - acute kidney injury KW - dogs KW - hypotension KW - ischaemia SP - 262 EP - 70 JF - Veterinary anaesthesia and analgesia JO - Vet Anaesth Analg VL - 43 IS - 3 N2 - OBJECTIVE: To determine changes in urine neutrophil gelatinase-associated lipocalin concentration (uNGAL) in anaesthetized Greyhound dogs that developed acute tubular damage following haemorrhage and resuscitation with colloid-based fluids. STUDY DESIGN: Prospective experimental study. ANIMALS: Seven healthy adult entire male Greyhound dogs. METHODS: During isoflurane anaesthesia, approximately 50 mL kg(-1) of blood was removed to maintain mean arterial pressure (MAP) ≤40 mmHg for 1 hour followed by gelatin-based colloid administration to maintain MAP ≥60 mmHg for 3 hours. Data, including oxygen extraction ratio and uNGAL, were collected before (T0) and immediately following (T1) haemorrhage, and hourly during reperfusion (T2-T4). After T4, dogs were euthanized and renal tissue was collected for histology. Statistical analysis was performed using repeated-measures one-way anova. Data are presented as means (95% confidence interval). RESULTS: Histology identified renal tubular epithelial damage in all dogs. Urine NGAL concentration increased from 12.1 (0-30.6) ng mL(-1) at T0 to 122.0 (64.1-180.0) ng mL(-1) by T3. Compared with T0, uNGAL was significantly higher at T3 (p = 0.016) and was increased 24-fold. CONCLUSIONS AND CLINICAL RELEVANCE: Despite wide individual variation in baseline uNGAL, increases in uNGAL were observed in all dogs, suggesting that this biomarker has the potential to detect renal tubular injury following haemorrhage-induced hypotension and colloid-mediated reperfusion. SN - 1467-2995 UR - https://www.unboundmedicine.com/medline/citation/26469528/Urinary_neutrophil_gelatinase_associated_lipocalin_concentration_changes_after_acute_haemorrhage_and_colloid_mediated_reperfusion_in_anaesthetized_dogs_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1467-2987(16)30070-8 DB - PRIME DP - Unbound Medicine ER -