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[Detection of Middle East Respiratory Syndrome Coronavirus by Reverse-transcription Loop-Mediated Isothermal Amplification].
Bing Du Xue Bao. 2015 May; 31(3):269-75.BD

Abstract

A simple, rapid and sensitive colorimetric reverse-transcription loop-mediated isothermal amplification (RT-LAMP) was developed for rapid detection of Middle East respiratory syndrome coronavirus (MERS-CoV). The method employed six primers that recognized sequences of a nucleocapsid gene for amplification of nucleic acids under isothermal conditions at 63 degrees C for 60 min. Products were detected through a LA-320c Loopamp Turbidimeter (real-time RT-LAMP) or visual inspection of color change by pre-addition of Hydroxynaphthol Blue dye (visual RT-LAMP). Specificity of RT-LAMP was validated by detection of several human coronaviruses and common respiratory viruses. MERS-CoV real-time RT-LAMP had a linear correlation (R2) of 0.995 at 10(3)-10(6) copies. The limit of detection of real-time RT-LAMP, visual RT-LAMP and quantitative real-time PCR was 500, 1000 and 100 copies/reaction, respectively. The established RT-LAMP assay was demonstrated to be a rapid screening tool for MERS-CoV infection, and could be suitable in resource-limited clinical sites and for field studies.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

26470533

Citation

Li, Guan, et al. "[Detection of Middle East Respiratory Syndrome Coronavirus By Reverse-transcription Loop-Mediated Isothermal Amplification]." Bing Du Xue Bao = Chinese Journal of Virology, vol. 31, no. 3, 2015, pp. 269-75.
Li G, Nie K, Zhang D, et al. [Detection of Middle East Respiratory Syndrome Coronavirus by Reverse-transcription Loop-Mediated Isothermal Amplification]. Bing Du Xue Bao. 2015;31(3):269-75.
Li, G., Nie, K., Zhang, D., Li, X., Wang, Y., Tan, W., & Ma, X. (2015). [Detection of Middle East Respiratory Syndrome Coronavirus by Reverse-transcription Loop-Mediated Isothermal Amplification]. Bing Du Xue Bao = Chinese Journal of Virology, 31(3), 269-75.
Li G, et al. [Detection of Middle East Respiratory Syndrome Coronavirus By Reverse-transcription Loop-Mediated Isothermal Amplification]. Bing Du Xue Bao. 2015;31(3):269-75. PubMed PMID: 26470533.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Detection of Middle East Respiratory Syndrome Coronavirus by Reverse-transcription Loop-Mediated Isothermal Amplification]. AU - Li,Guan, AU - Nie,Kai, AU - Zhang,Dan, AU - Li,Xinna, AU - Wang,Yanqun, AU - Tan,Wenjie, AU - Ma,Xuejun, PY - 2015/10/17/entrez PY - 2015/10/17/pubmed PY - 2015/11/13/medline SP - 269 EP - 75 JF - Bing du xue bao = Chinese journal of virology JO - Bing Du Xue Bao VL - 31 IS - 3 N2 - A simple, rapid and sensitive colorimetric reverse-transcription loop-mediated isothermal amplification (RT-LAMP) was developed for rapid detection of Middle East respiratory syndrome coronavirus (MERS-CoV). The method employed six primers that recognized sequences of a nucleocapsid gene for amplification of nucleic acids under isothermal conditions at 63 degrees C for 60 min. Products were detected through a LA-320c Loopamp Turbidimeter (real-time RT-LAMP) or visual inspection of color change by pre-addition of Hydroxynaphthol Blue dye (visual RT-LAMP). Specificity of RT-LAMP was validated by detection of several human coronaviruses and common respiratory viruses. MERS-CoV real-time RT-LAMP had a linear correlation (R2) of 0.995 at 10(3)-10(6) copies. The limit of detection of real-time RT-LAMP, visual RT-LAMP and quantitative real-time PCR was 500, 1000 and 100 copies/reaction, respectively. The established RT-LAMP assay was demonstrated to be a rapid screening tool for MERS-CoV infection, and could be suitable in resource-limited clinical sites and for field studies. SN - 1000-8721 UR - https://www.unboundmedicine.com/medline/citation/26470533/[Detection_of_Middle_East_Respiratory_Syndrome_Coronavirus_by_Reverse_transcription_Loop_Mediated_Isothermal_Amplification]_ L2 - https://antibodies.cancer.gov/detail/CPTC-RRM2-2 DB - PRIME DP - Unbound Medicine ER -