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Anti-steatotic effects of an n-3 LCPUFA and extra virgin olive oil mixture in the liver of mice subjected to high-fat diet.
Food Funct. 2016 Jan; 7(1):140-50.FF

Abstract

Non-alcoholic fatty liver disease (NAFLD) is characterized by liver steatosis, oxidative stress, and drastic depletion of n-3 long-chain polyunsaturated fatty acids (n-3 LCPUFA), namely, eicosapentaenoic acid (C20:5 n-3, EPA) and docosahexaenoic acid (C22:6 n-3, DHA), which trigger lipolysis stimulation and lipogenesis inhibition. Extra virgin olive oil (EVOO) has important antioxidant effects. This study evaluated the anti-steatotic effects of n-3 LCPUFA plus EVOO in the liver of male C57BL/6J mice subjected to a control diet (CD) (10% fat, 20% protein, 70% carbohydrate) or high fat diet (HFD) (60% fat, 20% protein, 20% carbohydrate), without and with supplementation with n-3 LCPUFA (100 mg per kg per day) plus EVOO (100 mg per kg per day) for 12 weeks. HFD induced (i) liver steatosis (increased total fat, triacylglycerols, and free fatty acid total contents), (ii) higher fasting serum glucose and insulin levels and HOMA index, total cholesterol, triacylglycerols and TNF-α and IL-6, (iii) liver and plasma oxidative stress enhancement, (iv) depletion of the n-3 LCPUFA hepatic content, and (v) increment in lipogenic enzyme activity and reduction in lipolytic enzyme activity. These changes were either reduced (p < 0.05) or normalized to control the values in animals subjected to HFD supplemented with n-3 LCPUFA plus EVOO. In conclusion, n-3 LCPUFA plus EVOO intervention exerts anti-steatotic effects underlying antioxidant and anti-inflammatory responses, improved insulin sensitivity, and recovery of the lipolytic/lipogenic status of the liver altered by HFD, and supports the potential therapeutic use of n-3 LCPUFA plus EVOO supplementation in the treatment of human liver steatosis induced by nutritional factors or other etiologies.

Authors+Show Affiliations

Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile. rvalenzuelab@med.uchile.cl.Medical Technology Department, Faculty of Medicine, University of Chile, Santiago, Chile.Institute for Research in Dental Sciences, Faculty of Dentistry, University of Chile, Santiago, Chile.Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile. rvalenzuelab@med.uchile.cl.Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile. rvalenzuelab@med.uchile.cl.Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile. rvalenzuelab@med.uchile.cl.Faculty of Chemical Sciences and Pharmacy, Department of Food Science and Chemical Technology, University of Chile, Santiago, Chile.Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile. rvalenzuelab@med.uchile.cl.Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile. rvalenzuelab@med.uchile.cl.Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26471014

Citation

Valenzuela, Rodrigo, et al. "Anti-steatotic Effects of an N-3 LCPUFA and Extra Virgin Olive Oil Mixture in the Liver of Mice Subjected to High-fat Diet." Food & Function, vol. 7, no. 1, 2016, pp. 140-50.
Valenzuela R, Espinosa A, Llanos P, et al. Anti-steatotic effects of an n-3 LCPUFA and extra virgin olive oil mixture in the liver of mice subjected to high-fat diet. Food Funct. 2016;7(1):140-50.
Valenzuela, R., Espinosa, A., Llanos, P., Hernandez-Rodas, M. C., Barrera, C., Vergara, D., Romero, N., Pérez, F., Ruz, M., & Videla, L. A. (2016). Anti-steatotic effects of an n-3 LCPUFA and extra virgin olive oil mixture in the liver of mice subjected to high-fat diet. Food & Function, 7(1), 140-50. https://doi.org/10.1039/c5fo01086a
Valenzuela R, et al. Anti-steatotic Effects of an N-3 LCPUFA and Extra Virgin Olive Oil Mixture in the Liver of Mice Subjected to High-fat Diet. Food Funct. 2016;7(1):140-50. PubMed PMID: 26471014.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-steatotic effects of an n-3 LCPUFA and extra virgin olive oil mixture in the liver of mice subjected to high-fat diet. AU - Valenzuela,Rodrigo, AU - Espinosa,Alejandra, AU - Llanos,Paola, AU - Hernandez-Rodas,Maria Catalina, AU - Barrera,Cynthia, AU - Vergara,Daniela, AU - Romero,Nalda, AU - Pérez,Francisco, AU - Ruz,Manuel, AU - Videla,Luis A, PY - 2015/10/17/entrez PY - 2015/10/17/pubmed PY - 2016/10/19/medline SP - 140 EP - 50 JF - Food & function JO - Food Funct VL - 7 IS - 1 N2 - Non-alcoholic fatty liver disease (NAFLD) is characterized by liver steatosis, oxidative stress, and drastic depletion of n-3 long-chain polyunsaturated fatty acids (n-3 LCPUFA), namely, eicosapentaenoic acid (C20:5 n-3, EPA) and docosahexaenoic acid (C22:6 n-3, DHA), which trigger lipolysis stimulation and lipogenesis inhibition. Extra virgin olive oil (EVOO) has important antioxidant effects. This study evaluated the anti-steatotic effects of n-3 LCPUFA plus EVOO in the liver of male C57BL/6J mice subjected to a control diet (CD) (10% fat, 20% protein, 70% carbohydrate) or high fat diet (HFD) (60% fat, 20% protein, 20% carbohydrate), without and with supplementation with n-3 LCPUFA (100 mg per kg per day) plus EVOO (100 mg per kg per day) for 12 weeks. HFD induced (i) liver steatosis (increased total fat, triacylglycerols, and free fatty acid total contents), (ii) higher fasting serum glucose and insulin levels and HOMA index, total cholesterol, triacylglycerols and TNF-α and IL-6, (iii) liver and plasma oxidative stress enhancement, (iv) depletion of the n-3 LCPUFA hepatic content, and (v) increment in lipogenic enzyme activity and reduction in lipolytic enzyme activity. These changes were either reduced (p < 0.05) or normalized to control the values in animals subjected to HFD supplemented with n-3 LCPUFA plus EVOO. In conclusion, n-3 LCPUFA plus EVOO intervention exerts anti-steatotic effects underlying antioxidant and anti-inflammatory responses, improved insulin sensitivity, and recovery of the lipolytic/lipogenic status of the liver altered by HFD, and supports the potential therapeutic use of n-3 LCPUFA plus EVOO supplementation in the treatment of human liver steatosis induced by nutritional factors or other etiologies. SN - 2042-650X UR - https://www.unboundmedicine.com/medline/citation/26471014/Anti_steatotic_effects_of_an_n_3_LCPUFA_and_extra_virgin_olive_oil_mixture_in_the_liver_of_mice_subjected_to_high_fat_diet_ L2 - https://doi.org/10.1039/c5fo01086a DB - PRIME DP - Unbound Medicine ER -