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Shedding light on microbial dark matter: a TM6 bacterium as natural endosymbiont of a free-living amoeba.
Environ Microbiol Rep. 2015 Dec; 7(6):970-8.EM

Abstract

The TM6 phylum belongs to the so-called microbial dark matter that gathers uncultivated bacteria detected only via DNA sequencing. Recently, the genome sequence of a TM6 bacterium (TM6SC1) has led to suggest that this bacterium would adopt an endosymbiotic life. In the present paper, free-living amoebae bearing a TM6 strain were isolated from a water network. The amoebae were identified as Vermamoeba vermiformis and the presence of a TM6 strain was detected by polymerase chain reaction and microscopy. The partial sequence of its 16S rRNA gene showed this strain to be closely related to the sequenced TM6SC1 strain. These bacteria displayed a pyriform shape and were found within V. vermiformis. Therefore, these bacteria were named Vermiphilus pyriformis. Interactions studies showed that V. pyriformis was highly infectious and that its relation with V. vermiformis was specific and highly stable. Finally, it was found that V. pyriformis inhibited the encystment of V. vermiformis. Overall, this study describes for the first time an endosymbiotic relationship between a TM6 bacterium and a free-living amoeba in the environment. It suggests that other bacteria of the TM6 phylum might also be endosymbiotic bacteria and may be found in other free-living amoebae or other organisms.

Authors+Show Affiliations

Microbiologie de l'Eau & Ecologie, Evolution, Symbiose, Laboratoire Ecologie et Biologie des Interactions, UMR CNRS 7267, Equipes, Université de Poitiers, Poitiers, France. Direction de la Recherche et du Développement pour la Qualité de l'Eau, R&D Biologie, Eau de Paris, 33, avenue Jean Jaurès, 94200, Ivry sur Seine, Paris, France.Microbiologie de l'Eau & Ecologie, Evolution, Symbiose, Laboratoire Ecologie et Biologie des Interactions, UMR CNRS 7267, Equipes, Université de Poitiers, Poitiers, France.Microbiologie de l'Eau & Ecologie, Evolution, Symbiose, Laboratoire Ecologie et Biologie des Interactions, UMR CNRS 7267, Equipes, Université de Poitiers, Poitiers, France.Direction de la Recherche et du Développement pour la Qualité de l'Eau, R&D Biologie, Eau de Paris, 33, avenue Jean Jaurès, 94200, Ivry sur Seine, Paris, France.Microbiologie de l'Eau & Ecologie, Evolution, Symbiose, Laboratoire Ecologie et Biologie des Interactions, UMR CNRS 7267, Equipes, Université de Poitiers, Poitiers, France.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26471960

Citation

Delafont, Vincent, et al. "Shedding Light On Microbial Dark Matter: a TM6 Bacterium as Natural Endosymbiont of a Free-living Amoeba." Environmental Microbiology Reports, vol. 7, no. 6, 2015, pp. 970-8.
Delafont V, Samba-Louaka A, Bouchon D, et al. Shedding light on microbial dark matter: a TM6 bacterium as natural endosymbiont of a free-living amoeba. Environ Microbiol Rep. 2015;7(6):970-8.
Delafont, V., Samba-Louaka, A., Bouchon, D., Moulin, L., & Héchard, Y. (2015). Shedding light on microbial dark matter: a TM6 bacterium as natural endosymbiont of a free-living amoeba. Environmental Microbiology Reports, 7(6), 970-8. https://doi.org/10.1111/1758-2229.12343
Delafont V, et al. Shedding Light On Microbial Dark Matter: a TM6 Bacterium as Natural Endosymbiont of a Free-living Amoeba. Environ Microbiol Rep. 2015;7(6):970-8. PubMed PMID: 26471960.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Shedding light on microbial dark matter: a TM6 bacterium as natural endosymbiont of a free-living amoeba. AU - Delafont,Vincent, AU - Samba-Louaka,Ascel, AU - Bouchon,Didier, AU - Moulin,Laurent, AU - Héchard,Yann, PY - 2015/07/20/received PY - 2015/10/06/revised PY - 2015/10/07/accepted PY - 2015/10/17/entrez PY - 2015/10/17/pubmed PY - 2016/10/14/medline SP - 970 EP - 8 JF - Environmental microbiology reports JO - Environ Microbiol Rep VL - 7 IS - 6 N2 - The TM6 phylum belongs to the so-called microbial dark matter that gathers uncultivated bacteria detected only via DNA sequencing. Recently, the genome sequence of a TM6 bacterium (TM6SC1) has led to suggest that this bacterium would adopt an endosymbiotic life. In the present paper, free-living amoebae bearing a TM6 strain were isolated from a water network. The amoebae were identified as Vermamoeba vermiformis and the presence of a TM6 strain was detected by polymerase chain reaction and microscopy. The partial sequence of its 16S rRNA gene showed this strain to be closely related to the sequenced TM6SC1 strain. These bacteria displayed a pyriform shape and were found within V. vermiformis. Therefore, these bacteria were named Vermiphilus pyriformis. Interactions studies showed that V. pyriformis was highly infectious and that its relation with V. vermiformis was specific and highly stable. Finally, it was found that V. pyriformis inhibited the encystment of V. vermiformis. Overall, this study describes for the first time an endosymbiotic relationship between a TM6 bacterium and a free-living amoeba in the environment. It suggests that other bacteria of the TM6 phylum might also be endosymbiotic bacteria and may be found in other free-living amoebae or other organisms. SN - 1758-2229 UR - https://www.unboundmedicine.com/medline/citation/26471960/Shedding_light_on_microbial_dark_matter:_a_TM6_bacterium_as_natural_endosymbiont_of_a_free_living_amoeba_ L2 - https://doi.org/10.1111/1758-2229.12343 DB - PRIME DP - Unbound Medicine ER -