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Bisphenol A suppresses Th1-type immune response in human peripheral blood mononuclear cells in vitro.
Immunol Lett. 2015 Dec; 168(2):285-92.IL

Abstract

Bisphenol A (BPA) is a widely used plasticizer, which came into focus because of its genotoxic and sensitizing potential. Besides its toxic properties, BPA is also well-known for its antioxidant chemical properties. This in vitro study investigated the interference of BPA with interferon-γ (IFN-γ)-induced tryptophan breakdown and neopterin production in human peripheral blood mononuclear cells (PBMC). The pro-inflammatory cytokine IFN-γ induces the conversion of the essential amino acid tryptophan into kynurenine via the enzyme indoleamine-2,3-dioxygenase (IDO-1). In parallel, GTP-cyclohydrolase produces neopterin, a marker of immune activation. A model system of phytohaemagglutinin (PHA)-stimulated PBMC was used to assess potential immunomodulatory properties of BPA. Treatment of cells with BPA [12.5-200μM] resulted in a significant and dose-dependent suppression of mitogen-induced tryptophan breakdown and neopterin formation along with a decrease of IFN-γ levels. Similar but less pronounced effects were observed in unstimulated cells. We postulate that the inhibitory effects of BPA on both T-cell activation and IDO-1 activity that we describe here may be critical for immune surveillance and is likely to influence T helper (Th) type 1/Th2 balance. Such immunosuppressive effects likely contribute to counteract inflammation. Further studies are required to address the in vivo relevance our in vitro findings.

Authors+Show Affiliations

Division of Medical Biochemistry, Biocenter, Medical University of Innsbruck, Innrain 80, 6020 Innsbruck, Austria; Receptor Biology Group, Inflammation, Repair and Development Section, National Heart and Lung Institute, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.Division of Biological Chemistry, Biocenter, Medical University of Innsbruck, Innrain 80, 6020 Innsbruck, Austria.Division of Biological Chemistry, Biocenter, Medical University of Innsbruck, Innrain 80, 6020 Innsbruck, Austria.Division of Medical Biochemistry, Biocenter, Medical University of Innsbruck, Innrain 80, 6020 Innsbruck, Austria.Central Institute of Blood Transfusion and Immunology, University Hospital, Anichstrasse 35, 6020 Innsbruck, Austria.Receptor Biology Group, Inflammation, Repair and Development Section, National Heart and Lung Institute, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.Division of Biological Chemistry, Biocenter, Medical University of Innsbruck, Innrain 80, 6020 Innsbruck, Austria. Electronic address: dietmar.fuchs@i-med.ac.at.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26475400

Citation

Gostner, Johanna M., et al. "Bisphenol a Suppresses Th1-type Immune Response in Human Peripheral Blood Mononuclear Cells in Vitro." Immunology Letters, vol. 168, no. 2, 2015, pp. 285-92.
Gostner JM, Raggl E, Becker K, et al. Bisphenol A suppresses Th1-type immune response in human peripheral blood mononuclear cells in vitro. Immunol Lett. 2015;168(2):285-92.
Gostner, J. M., Raggl, E., Becker, K., Überall, F., Schennach, H., Pease, J. E., & Fuchs, D. (2015). Bisphenol A suppresses Th1-type immune response in human peripheral blood mononuclear cells in vitro. Immunology Letters, 168(2), 285-92. https://doi.org/10.1016/j.imlet.2015.10.006
Gostner JM, et al. Bisphenol a Suppresses Th1-type Immune Response in Human Peripheral Blood Mononuclear Cells in Vitro. Immunol Lett. 2015;168(2):285-92. PubMed PMID: 26475400.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bisphenol A suppresses Th1-type immune response in human peripheral blood mononuclear cells in vitro. AU - Gostner,Johanna M, AU - Raggl,Emanuel, AU - Becker,Kathrin, AU - Überall,Florian, AU - Schennach,Harald, AU - Pease,James E, AU - Fuchs,Dietmar, Y1 - 2015/10/22/ PY - 2015/06/29/received PY - 2015/10/06/revised PY - 2015/10/08/accepted PY - 2015/10/18/entrez PY - 2015/10/18/pubmed PY - 2016/9/28/medline KW - Bisphenol A KW - Human PBMC KW - Indoleamine 2,3-dioxygenase KW - Neopterin KW - Tryptophan SP - 285 EP - 92 JF - Immunology letters JO - Immunol. Lett. VL - 168 IS - 2 N2 - Bisphenol A (BPA) is a widely used plasticizer, which came into focus because of its genotoxic and sensitizing potential. Besides its toxic properties, BPA is also well-known for its antioxidant chemical properties. This in vitro study investigated the interference of BPA with interferon-γ (IFN-γ)-induced tryptophan breakdown and neopterin production in human peripheral blood mononuclear cells (PBMC). The pro-inflammatory cytokine IFN-γ induces the conversion of the essential amino acid tryptophan into kynurenine via the enzyme indoleamine-2,3-dioxygenase (IDO-1). In parallel, GTP-cyclohydrolase produces neopterin, a marker of immune activation. A model system of phytohaemagglutinin (PHA)-stimulated PBMC was used to assess potential immunomodulatory properties of BPA. Treatment of cells with BPA [12.5-200μM] resulted in a significant and dose-dependent suppression of mitogen-induced tryptophan breakdown and neopterin formation along with a decrease of IFN-γ levels. Similar but less pronounced effects were observed in unstimulated cells. We postulate that the inhibitory effects of BPA on both T-cell activation and IDO-1 activity that we describe here may be critical for immune surveillance and is likely to influence T helper (Th) type 1/Th2 balance. Such immunosuppressive effects likely contribute to counteract inflammation. Further studies are required to address the in vivo relevance our in vitro findings. SN - 1879-0542 UR - https://www.unboundmedicine.com/medline/citation/26475400/Bisphenol_A_suppresses_Th1_type_immune_response_in_human_peripheral_blood_mononuclear_cells_in_vitro_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-2478(15)30047-X DB - PRIME DP - Unbound Medicine ER -